Experiment: KB with Strychnine Sulfate 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0014 and Psyr_0015 are separated by 339 nucleotides Psyr_0015 and Psyr_0016 are separated by 153 nucleotides
Psyr_0014: Psyr_0014 - lipid A biosynthesis acyltransferase, at 15,585 to 16,472
_0014
Psyr_0015: Psyr_0015 - Oxidoreductase alpha (molybdopterin) subunit, at 16,812 to 19,175
_0015
Psyr_0016: Psyr_0016 - TrkA-N:TrkA-C, at 19,329 to 20,702
_0016
Position (kb) 16
17
18
19
20 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 15.976 kb on - strand, within Psyr_0014 at 15.976 kb on - strand, within Psyr_0014 at 15.976 kb on - strand, within Psyr_0014 at 15.976 kb on - strand, within Psyr_0014 at 16.300 kb on - strand, within Psyr_0014 at 16.301 kb on + strand, within Psyr_0014 at 16.301 kb on + strand, within Psyr_0014 at 16.301 kb on + strand, within Psyr_0014 at 16.301 kb on + strand, within Psyr_0014 at 16.302 kb on - strand, within Psyr_0014 at 16.302 kb on - strand, within Psyr_0014 at 16.302 kb on - strand, within Psyr_0014 at 16.335 kb on + strand, within Psyr_0014 at 16.335 kb on + strand, within Psyr_0014 at 16.336 kb on - strand, within Psyr_0014 at 16.336 kb on - strand, within Psyr_0014 at 16.389 kb on + strand at 16.389 kb on + strand at 16.390 kb on - strand at 16.390 kb on - strand at 16.413 kb on + strand at 16.413 kb on + strand at 16.470 kb on + strand at 16.470 kb on + strand at 16.471 kb on - strand at 16.471 kb on - strand at 16.471 kb on - strand at 16.471 kb on - strand at 16.549 kb on + strand at 16.550 kb on - strand at 16.550 kb on - strand at 16.550 kb on - strand at 16.632 kb on - strand at 16.701 kb on + strand at 16.701 kb on + strand at 16.701 kb on + strand at 16.701 kb on + strand at 16.701 kb on + strand at 16.702 kb on - strand at 16.702 kb on - strand at 16.779 kb on - strand at 16.779 kb on - strand at 16.914 kb on - strand at 16.944 kb on + strand at 16.944 kb on + strand at 17.068 kb on + strand, within Psyr_0015 at 17.068 kb on + strand, within Psyr_0015 at 17.068 kb on + strand, within Psyr_0015 at 17.069 kb on - strand, within Psyr_0015 at 17.069 kb on - strand, within Psyr_0015 at 17.069 kb on - strand, within Psyr_0015 at 17.069 kb on - strand, within Psyr_0015 at 17.069 kb on - strand, within Psyr_0015 at 17.167 kb on + strand, within Psyr_0015 at 17.167 kb on + strand, within Psyr_0015 at 17.167 kb on + strand, within Psyr_0015 at 17.168 kb on - strand, within Psyr_0015 at 17.168 kb on - strand, within Psyr_0015 at 17.168 kb on - strand, within Psyr_0015 at 17.251 kb on + strand, within Psyr_0015 at 17.252 kb on - strand, within Psyr_0015 at 17.252 kb on - strand, within Psyr_0015 at 17.252 kb on - strand, within Psyr_0015 at 17.252 kb on - strand, within Psyr_0015 at 17.256 kb on - strand, within Psyr_0015 at 17.256 kb on - strand, within Psyr_0015 at 17.368 kb on + strand, within Psyr_0015 at 17.368 kb on + strand, within Psyr_0015 at 17.369 kb on - strand, within Psyr_0015 at 17.369 kb on - strand, within Psyr_0015 at 17.385 kb on + strand, within Psyr_0015 at 17.385 kb on + strand, within Psyr_0015 at 17.385 kb on + strand, within Psyr_0015 at 17.386 kb on - strand, within Psyr_0015 at 17.417 kb on - strand, within Psyr_0015 at 17.428 kb on + strand, within Psyr_0015 at 17.428 kb on + strand, within Psyr_0015 at 17.428 kb on + strand, within Psyr_0015 at 17.428 kb on + strand, within Psyr_0015 at 17.428 kb on + strand, within Psyr_0015 at 17.428 kb on + strand, within Psyr_0015 at 17.428 kb on + strand, within Psyr_0015 at 17.428 kb on + strand, within Psyr_0015 at 17.428 kb on + strand, within Psyr_0015 at 17.428 kb on + strand, within Psyr_0015 at 17.428 kb on + strand, within Psyr_0015 at 17.428 kb on + strand, within Psyr_0015 at 17.428 kb on + strand, within Psyr_0015 at 17.428 kb on + strand, within Psyr_0015 at 17.428 kb on + strand, within Psyr_0015 at 17.428 kb on + strand, within Psyr_0015 at 17.428 kb on + strand, within Psyr_0015 at 17.428 kb on + strand, within Psyr_0015 at 17.428 kb on + strand, within Psyr_0015 at 17.428 kb on + strand, within Psyr_0015 at 17.428 kb on + strand, within Psyr_0015 at 17.428 kb on + strand, within Psyr_0015 at 17.428 kb on + strand, within Psyr_0015 at 17.428 kb on + strand, within Psyr_0015 at 17.428 kb on + strand, within Psyr_0015 at 17.429 kb on - strand, within Psyr_0015 at 17.429 kb on - strand, within Psyr_0015 at 17.429 kb on - strand, within Psyr_0015 at 17.429 kb on - strand, within Psyr_0015 at 17.429 kb on - strand, within Psyr_0015 at 17.429 kb on - strand, within Psyr_0015 at 17.429 kb on - strand, within Psyr_0015 at 17.429 kb on - strand, within Psyr_0015 at 17.429 kb on - strand, within Psyr_0015 at 17.429 kb on - strand, within Psyr_0015 at 17.429 kb on - strand, within Psyr_0015 at 17.429 kb on - strand, within Psyr_0015 at 17.429 kb on - strand, within Psyr_0015 at 17.429 kb on - strand, within Psyr_0015 at 17.429 kb on - strand, within Psyr_0015 at 17.429 kb on - strand, within Psyr_0015 at 17.429 kb on - strand, within Psyr_0015 at 17.429 kb on - strand, within Psyr_0015 at 17.429 kb on - strand, within Psyr_0015 at 17.574 kb on - strand, within Psyr_0015 at 17.899 kb on + strand, within Psyr_0015 at 17.899 kb on + strand, within Psyr_0015 at 17.925 kb on + strand, within Psyr_0015 at 17.925 kb on + strand, within Psyr_0015 at 17.925 kb on + strand, within Psyr_0015 at 17.925 kb on + strand, within Psyr_0015 at 17.926 kb on - strand, within Psyr_0015 at 17.926 kb on - strand, within Psyr_0015 at 17.926 kb on - strand, within Psyr_0015 at 17.926 kb on - strand, within Psyr_0015 at 17.926 kb on - strand, within Psyr_0015 at 17.986 kb on - strand, within Psyr_0015 at 18.084 kb on - strand, within Psyr_0015 at 18.085 kb on + strand, within Psyr_0015 at 18.085 kb on + strand, within Psyr_0015 at 18.085 kb on + strand, within Psyr_0015 at 18.085 kb on + strand, within Psyr_0015 at 18.085 kb on + strand, within Psyr_0015 at 18.086 kb on - strand, within Psyr_0015 at 18.086 kb on - strand, within Psyr_0015 at 18.086 kb on - strand, within Psyr_0015 at 18.086 kb on - strand, within Psyr_0015 at 18.086 kb on - strand, within Psyr_0015 at 18.222 kb on + strand, within Psyr_0015 at 18.222 kb on + strand, within Psyr_0015 at 18.223 kb on - strand, within Psyr_0015 at 18.223 kb on - strand, within Psyr_0015 at 18.223 kb on - strand, within Psyr_0015 at 18.325 kb on + strand, within Psyr_0015 at 18.325 kb on + strand, within Psyr_0015 at 18.325 kb on + strand, within Psyr_0015 at 18.640 kb on + strand, within Psyr_0015 at 18.641 kb on - strand, within Psyr_0015 at 18.641 kb on - strand, within Psyr_0015 at 18.641 kb on - strand, within Psyr_0015 at 18.641 kb on - strand, within Psyr_0015 at 18.661 kb on + strand, within Psyr_0015 at 18.662 kb on - strand, within Psyr_0015 at 18.700 kb on + strand, within Psyr_0015 at 18.700 kb on + strand, within Psyr_0015 at 18.700 kb on + strand, within Psyr_0015 at 18.700 kb on + strand, within Psyr_0015 at 18.700 kb on + strand, within Psyr_0015 at 18.700 kb on + strand, within Psyr_0015 at 18.700 kb on + strand, within Psyr_0015 at 18.700 kb on + strand, within Psyr_0015 at 18.700 kb on + strand, within Psyr_0015 at 18.701 kb on - strand, within Psyr_0015 at 18.701 kb on - strand, within Psyr_0015 at 18.701 kb on - strand, within Psyr_0015 at 18.784 kb on + strand, within Psyr_0015 at 18.784 kb on + strand, within Psyr_0015 at 18.785 kb on - strand, within Psyr_0015 at 18.785 kb on - strand, within Psyr_0015 at 18.785 kb on - strand, within Psyr_0015 at 18.785 kb on - strand, within Psyr_0015 at 18.805 kb on + strand, within Psyr_0015 at 18.878 kb on + strand, within Psyr_0015 at 18.879 kb on - strand, within Psyr_0015 at 18.955 kb on + strand at 18.955 kb on + strand at 18.955 kb on + strand at 19.194 kb on + strand at 19.194 kb on + strand at 19.195 kb on - strand at 19.195 kb on - strand at 19.195 kb on - strand at 19.195 kb on - strand at 19.231 kb on + strand at 19.231 kb on + strand at 19.231 kb on + strand at 19.231 kb on + strand at 19.231 kb on + strand at 19.232 kb on - strand at 19.232 kb on - strand at 19.389 kb on + strand at 19.389 kb on + strand at 19.390 kb on - strand at 19.703 kb on - strand, within Psyr_0016 at 19.703 kb on - strand, within Psyr_0016 at 19.901 kb on - strand, within Psyr_0016 at 20.003 kb on + strand, within Psyr_0016 at 20.003 kb on + strand, within Psyr_0016 at 20.003 kb on + strand, within Psyr_0016 at 20.011 kb on + strand, within Psyr_0016 at 20.011 kb on + strand, within Psyr_0016 at 20.011 kb on + strand, within Psyr_0016 at 20.011 kb on + strand, within Psyr_0016 at 20.012 kb on - strand, within Psyr_0016 at 20.012 kb on - strand, within Psyr_0016 at 20.012 kb on - strand, within Psyr_0016 at 20.012 kb on - strand, within Psyr_0016 at 20.012 kb on - strand, within Psyr_0016 at 20.012 kb on - strand, within Psyr_0016 at 20.012 kb on - strand, within Psyr_0016 at 20.012 kb on - strand, within Psyr_0016 at 20.012 kb on - strand, within Psyr_0016
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Strychnine Sulfate 10 mM remove 15,976 - Psyr_0014 0.44 -1.4 15,976 - Psyr_0014 0.44 -1.1 15,976 - Psyr_0014 0.44 -0.1 15,976 - Psyr_0014 0.44 -1.6 16,300 - Psyr_0014 0.81 -0.9 16,301 + Psyr_0014 0.81 -1.0 16,301 + Psyr_0014 0.81 -0.4 16,301 + Psyr_0014 0.81 -0.6 16,301 + Psyr_0014 0.81 -1.6 16,302 - Psyr_0014 0.81 +0.3 16,302 - Psyr_0014 0.81 -0.1 16,302 - Psyr_0014 0.81 -2.0 16,335 + Psyr_0014 0.84 -1.0 16,335 + Psyr_0014 0.84 -1.2 16,336 - Psyr_0014 0.85 -2.3 16,336 - Psyr_0014 0.85 -0.1 16,389 + -1.4 16,389 + -0.1 16,390 - -1.1 16,390 - -0.8 16,413 + -2.5 16,413 + +1.4 16,470 + -0.6 16,470 + +0.3 16,471 - +2.0 16,471 - -1.1 16,471 - +0.1 16,471 - -2.1 16,549 + -0.3 16,550 - +0.7 16,550 - +0.4 16,550 - +0.9 16,632 - +0.2 16,701 + -0.4 16,701 + +0.2 16,701 + +0.9 16,701 + -0.8 16,701 + -0.2 16,702 - +0.1 16,702 - +0.3 16,779 - +0.2 16,779 - -0.6 16,914 - +0.3 16,944 + +0.7 16,944 + -0.4 17,068 + Psyr_0015 0.11 -0.9 17,068 + Psyr_0015 0.11 -0.2 17,068 + Psyr_0015 0.11 -0.7 17,069 - Psyr_0015 0.11 -0.4 17,069 - Psyr_0015 0.11 -0.2 17,069 - Psyr_0015 0.11 +0.1 17,069 - Psyr_0015 0.11 +3.4 17,069 - Psyr_0015 0.11 +0.4 17,167 + Psyr_0015 0.15 -2.5 17,167 + Psyr_0015 0.15 +0.3 17,167 + Psyr_0015 0.15 -0.4 17,168 - Psyr_0015 0.15 +0.4 17,168 - Psyr_0015 0.15 +0.1 17,168 - Psyr_0015 0.15 -0.4 17,251 + Psyr_0015 0.19 -0.7 17,252 - Psyr_0015 0.19 +0.6 17,252 - Psyr_0015 0.19 +0.7 17,252 - Psyr_0015 0.19 +1.1 17,252 - Psyr_0015 0.19 -0.1 17,256 - Psyr_0015 0.19 -0.6 17,256 - Psyr_0015 0.19 +0.1 17,368 + Psyr_0015 0.24 -0.5 17,368 + Psyr_0015 0.24 +0.4 17,369 - Psyr_0015 0.24 +1.4 17,369 - Psyr_0015 0.24 +0.9 17,385 + Psyr_0015 0.24 +0.3 17,385 + Psyr_0015 0.24 +0.7 17,385 + Psyr_0015 0.24 +0.1 17,386 - Psyr_0015 0.24 -0.3 17,417 - Psyr_0015 0.26 +1.7 17,428 + Psyr_0015 0.26 +0.6 17,428 + Psyr_0015 0.26 +0.2 17,428 + Psyr_0015 0.26 -0.6 17,428 + Psyr_0015 0.26 +0.9 17,428 + Psyr_0015 0.26 -0.0 17,428 + Psyr_0015 0.26 -0.7 17,428 + Psyr_0015 0.26 +0.7 17,428 + Psyr_0015 0.26 -0.2 17,428 + Psyr_0015 0.26 +0.4 17,428 + Psyr_0015 0.26 +0.7 17,428 + Psyr_0015 0.26 +0.4 17,428 + Psyr_0015 0.26 -0.3 17,428 + Psyr_0015 0.26 +0.6 17,428 + Psyr_0015 0.26 -0.0 17,428 + Psyr_0015 0.26 -0.3 17,428 + Psyr_0015 0.26 -0.2 17,428 + Psyr_0015 0.26 +2.0 17,428 + Psyr_0015 0.26 -0.4 17,428 + Psyr_0015 0.26 +0.2 17,428 + Psyr_0015 0.26 -1.3 17,428 + Psyr_0015 0.26 +1.6 17,428 + Psyr_0015 0.26 -0.1 17,428 + Psyr_0015 0.26 -0.5 17,428 + Psyr_0015 0.26 +1.9 17,428 + Psyr_0015 0.26 +0.1 17,429 - Psyr_0015 0.26 -1.6 17,429 - Psyr_0015 0.26 +1.4 17,429 - Psyr_0015 0.26 -1.1 17,429 - Psyr_0015 0.26 +0.7 17,429 - Psyr_0015 0.26 -0.4 17,429 - Psyr_0015 0.26 -0.1 17,429 - Psyr_0015 0.26 +0.2 17,429 - Psyr_0015 0.26 +1.0 17,429 - Psyr_0015 0.26 -0.2 17,429 - Psyr_0015 0.26 -0.6 17,429 - Psyr_0015 0.26 -0.7 17,429 - Psyr_0015 0.26 -1.0 17,429 - Psyr_0015 0.26 +0.7 17,429 - Psyr_0015 0.26 -0.8 17,429 - Psyr_0015 0.26 -0.6 17,429 - Psyr_0015 0.26 +0.5 17,429 - Psyr_0015 0.26 -0.9 17,429 - Psyr_0015 0.26 +1.6 17,429 - Psyr_0015 0.26 +1.2 17,574 - Psyr_0015 0.32 +0.1 17,899 + Psyr_0015 0.46 +0.4 17,899 + Psyr_0015 0.46 -0.9 17,925 + Psyr_0015 0.47 +0.4 17,925 + Psyr_0015 0.47 -0.1 17,925 + Psyr_0015 0.47 -1.4 17,925 + Psyr_0015 0.47 -0.1 17,926 - Psyr_0015 0.47 -0.4 17,926 - Psyr_0015 0.47 +0.7 17,926 - Psyr_0015 0.47 -0.6 17,926 - Psyr_0015 0.47 -0.3 17,926 - Psyr_0015 0.47 +1.0 17,986 - Psyr_0015 0.50 +0.5 18,084 - Psyr_0015 0.54 -0.3 18,085 + Psyr_0015 0.54 -0.1 18,085 + Psyr_0015 0.54 -0.4 18,085 + Psyr_0015 0.54 +1.0 18,085 + Psyr_0015 0.54 -0.2 18,085 + Psyr_0015 0.54 -0.0 18,086 - Psyr_0015 0.54 -0.9 18,086 - Psyr_0015 0.54 -0.3 18,086 - Psyr_0015 0.54 +0.6 18,086 - Psyr_0015 0.54 +1.0 18,086 - Psyr_0015 0.54 +1.0 18,222 + Psyr_0015 0.60 -1.3 18,222 + Psyr_0015 0.60 -0.4 18,223 - Psyr_0015 0.60 -0.6 18,223 - Psyr_0015 0.60 +2.4 18,223 - Psyr_0015 0.60 -0.1 18,325 + Psyr_0015 0.64 -0.2 18,325 + Psyr_0015 0.64 -0.0 18,325 + Psyr_0015 0.64 -0.3 18,640 + Psyr_0015 0.77 +1.3 18,641 - Psyr_0015 0.77 -0.4 18,641 - Psyr_0015 0.77 +0.4 18,641 - Psyr_0015 0.77 +1.1 18,641 - Psyr_0015 0.77 -0.6 18,661 + Psyr_0015 0.78 +0.7 18,662 - Psyr_0015 0.78 +0.2 18,700 + Psyr_0015 0.80 -0.5 18,700 + Psyr_0015 0.80 +1.1 18,700 + Psyr_0015 0.80 -0.1 18,700 + Psyr_0015 0.80 +0.7 18,700 + Psyr_0015 0.80 +0.4 18,700 + Psyr_0015 0.80 -0.4 18,700 + Psyr_0015 0.80 -0.1 18,700 + Psyr_0015 0.80 +0.3 18,700 + Psyr_0015 0.80 +1.2 18,701 - Psyr_0015 0.80 +0.8 18,701 - Psyr_0015 0.80 -0.2 18,701 - Psyr_0015 0.80 +0.5 18,784 + Psyr_0015 0.83 +0.6 18,784 + Psyr_0015 0.83 -0.1 18,785 - Psyr_0015 0.83 +2.0 18,785 - Psyr_0015 0.83 +0.2 18,785 - Psyr_0015 0.83 +0.3 18,785 - Psyr_0015 0.83 +0.2 18,805 + Psyr_0015 0.84 -0.9 18,878 + Psyr_0015 0.87 +1.9 18,879 - Psyr_0015 0.87 -1.1 18,955 + -0.8 18,955 + +0.0 18,955 + -0.3 19,194 + -0.4 19,194 + +0.4 19,195 - -0.7 19,195 - +0.9 19,195 - -0.7 19,195 - -0.1 19,231 + +0.6 19,231 + -0.4 19,231 + -0.7 19,231 + -1.2 19,231 + -0.6 19,232 - +0.2 19,232 - +1.2 19,389 + +0.0 19,389 + -0.9 19,390 - -0.6 19,703 - Psyr_0016 0.27 +0.4 19,703 - Psyr_0016 0.27 -2.6 19,901 - Psyr_0016 0.42 -0.6 20,003 + Psyr_0016 0.49 -0.1 20,003 + Psyr_0016 0.49 +0.6 20,003 + Psyr_0016 0.49 -0.1 20,011 + Psyr_0016 0.50 +1.1 20,011 + Psyr_0016 0.50 -0.1 20,011 + Psyr_0016 0.50 -0.0 20,011 + Psyr_0016 0.50 -1.4 20,012 - Psyr_0016 0.50 +0.4 20,012 - Psyr_0016 0.50 -0.1 20,012 - Psyr_0016 0.50 -1.9 20,012 - Psyr_0016 0.50 +1.8 20,012 - Psyr_0016 0.50 -1.5 20,012 - Psyr_0016 0.50 -0.9 20,012 - Psyr_0016 0.50 -0.1 20,012 - Psyr_0016 0.50 +2.8 20,012 - Psyr_0016 0.50 -1.4
Or see this region's nucleotide sequence