Strain Fitness in Escherichia coli BW25113 around b3927
Experiment: LB Anaerobic with Spectinomycin 0.0000625 mM
Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.
Per-strain Table
Position | Strand | Gene | LocusTag | Fraction | LB Anaerobic with Spectinomycin 0.0000625 mM |
---|---|---|---|---|---|
remove | |||||
4,114,318 | + | glpK | b3926 | 0.39 | +1.0 |
4,114,342 | - | glpK | b3926 | 0.40 | -0.2 |
4,114,429 | + | glpK | b3926 | 0.46 | +0.4 |
4,114,429 | + | glpK | b3926 | 0.46 | -1.1 |
4,114,437 | - | glpK | b3926 | 0.46 | -0.0 |
4,114,437 | - | glpK | b3926 | 0.46 | -0.3 |
4,114,437 | - | glpK | b3926 | 0.46 | -0.3 |
4,114,437 | - | glpK | b3926 | 0.46 | +0.3 |
4,114,453 | - | glpK | b3926 | 0.47 | -1.5 |
4,114,474 | - | glpK | b3926 | 0.49 | -0.0 |
4,114,510 | - | glpK | b3926 | 0.51 | +0.4 |
4,114,539 | - | glpK | b3926 | 0.53 | +0.4 |
4,114,557 | + | glpK | b3926 | 0.54 | +1.2 |
4,114,557 | + | glpK | b3926 | 0.54 | +1.0 |
4,114,569 | - | glpK | b3926 | 0.55 | -0.6 |
4,114,656 | + | glpK | b3926 | 0.61 | +1.4 |
4,114,657 | - | glpK | b3926 | 0.61 | -1.0 |
4,114,664 | + | glpK | b3926 | 0.61 | +1.0 |
4,114,664 | + | glpK | b3926 | 0.61 | +0.0 |
4,114,684 | - | glpK | b3926 | 0.63 | -0.5 |
4,114,684 | - | glpK | b3926 | 0.63 | -1.5 |
4,114,703 | + | glpK | b3926 | 0.64 | -2.0 |
4,114,703 | + | glpK | b3926 | 0.64 | -0.2 |
4,114,710 | + | glpK | b3926 | 0.64 | +1.0 |
4,114,710 | + | glpK | b3926 | 0.64 | +1.0 |
4,114,723 | - | glpK | b3926 | 0.65 | -0.2 |
4,114,723 | - | glpK | b3926 | 0.65 | +0.2 |
4,114,738 | + | glpK | b3926 | 0.66 | +0.7 |
4,114,743 | + | glpK | b3926 | 0.67 | +0.4 |
4,114,750 | - | glpK | b3926 | 0.67 | -0.7 |
4,114,770 | - | glpK | b3926 | 0.68 | +0.6 |
4,114,775 | + | glpK | b3926 | 0.69 | +0.6 |
4,114,777 | + | glpK | b3926 | 0.69 | +0.3 |
4,114,784 | + | glpK | b3926 | 0.69 | -0.5 |
4,114,794 | - | glpK | b3926 | 0.70 | +0.4 |
4,114,814 | + | glpK | b3926 | 0.71 | +0.1 |
4,114,817 | + | glpK | b3926 | 0.72 | +0.2 |
4,114,833 | + | glpK | b3926 | 0.73 | -0.1 |
4,114,870 | + | glpK | b3926 | 0.75 | -1.5 |
4,114,887 | - | -1.0 | |||
4,114,939 | - | glpK | b3926 | 0.80 | -0.1 |
4,114,939 | - | glpK | b3926 | 0.80 | -0.4 |
4,114,951 | + | glpK | b3926 | 0.80 | +0.9 |
4,114,951 | + | glpK | b3926 | 0.80 | +0.0 |
4,114,952 | - | glpK | b3926 | 0.81 | +0.7 |
4,114,989 | - | glpK | b3926 | 0.83 | -1.2 |
4,114,989 | - | glpK | b3926 | 0.83 | +0.1 |
4,115,009 | + | glpK | b3926 | 0.84 | -0.1 |
4,115,009 | + | glpK | b3926 | 0.84 | +1.9 |
4,115,010 | - | glpK | b3926 | 0.84 | +0.0 |
4,115,010 | - | glpK | b3926 | 0.84 | -0.3 |
4,115,044 | - | glpK | b3926 | 0.87 | -0.5 |
4,115,055 | + | glpK | b3926 | 0.87 | -1.1 |
4,115,063 | - | glpK | b3926 | 0.88 | +0.5 |
4,115,063 | - | glpK | b3926 | 0.88 | -0.0 |
4,115,065 | + | glpK | b3926 | 0.88 | +0.9 |
4,115,065 | + | glpK | b3926 | 0.88 | -1.0 |
4,115,072 | + | glpK | b3926 | 0.88 | +0.1 |
4,115,073 | - | glpK | b3926 | 0.89 | -0.6 |
4,115,081 | + | glpK | b3926 | 0.89 | +0.0 |
4,115,087 | + | glpK | b3926 | 0.89 | +0.0 |
4,115,099 | + | -0.2 | |||
4,115,100 | - | -1.2 | |||
4,115,102 | + | -0.1 | |||
4,115,102 | + | +0.3 | |||
4,115,112 | - | +0.6 | |||
4,115,112 | - | -0.5 | |||
4,115,112 | - | +0.4 | |||
4,115,113 | + | +0.2 | |||
4,115,157 | - | +0.7 | |||
4,115,164 | - | -0.5 | |||
4,115,164 | - | -0.9 | |||
4,115,167 | - | +1.0 | |||
4,115,171 | + | -0.4 | |||
4,115,180 | + | +0.4 | |||
4,115,182 | - | +1.6 | |||
4,115,184 | - | -0.1 | |||
4,115,184 | - | -1.1 | |||
4,115,188 | - | +0.8 | |||
4,115,188 | - | +0.1 | |||
4,115,254 | - | +0.2 | |||
4,115,254 | - | +1.4 | |||
4,115,262 | + | +0.0 | |||
4,115,265 | + | -1.5 | |||
4,115,285 | + | +0.0 | |||
4,115,296 | + | +0.0 | |||
4,115,325 | + | +0.0 | |||
4,115,363 | + | glpF | b3927 | 0.11 | -0.8 |
4,115,363 | + | glpF | b3927 | 0.11 | -0.3 |
4,115,371 | + | glpF | b3927 | 0.12 | +0.0 |
4,115,381 | - | glpF | b3927 | 0.13 | -0.3 |
4,115,388 | + | glpF | b3927 | 0.14 | +0.7 |
4,115,415 | + | glpF | b3927 | 0.17 | +0.5 |
4,115,430 | - | glpF | b3927 | 0.19 | -0.2 |
4,115,431 | - | glpF | b3927 | 0.19 | +0.2 |
4,115,449 | + | glpF | b3927 | 0.21 | -1.7 |
4,115,462 | + | glpF | b3927 | 0.23 | +0.1 |
4,115,544 | + | glpF | b3927 | 0.33 | -0.7 |
4,115,544 | + | glpF | b3927 | 0.33 | +0.4 |
4,115,549 | - | glpF | b3927 | 0.33 | -1.1 |
4,115,552 | + | glpF | b3927 | 0.34 | +0.0 |
4,115,552 | + | glpF | b3927 | 0.34 | -2.0 |
4,115,564 | - | glpF | b3927 | 0.35 | +0.2 |
4,115,566 | - | glpF | b3927 | 0.35 | -0.5 |
4,115,577 | + | glpF | b3927 | 0.37 | -1.8 |
4,115,585 | - | glpF | b3927 | 0.37 | +0.5 |
4,115,585 | - | glpF | b3927 | 0.37 | +0.8 |
4,115,589 | + | glpF | b3927 | 0.38 | -2.3 |
4,115,589 | + | glpF | b3927 | 0.38 | +0.2 |
4,115,602 | - | glpF | b3927 | 0.39 | -0.2 |
4,115,602 | - | glpF | b3927 | 0.39 | +0.4 |
4,115,602 | - | glpF | b3927 | 0.39 | -0.3 |
4,115,602 | - | glpF | b3927 | 0.39 | +1.2 |
4,115,612 | - | glpF | b3927 | 0.41 | -1.3 |
4,115,612 | - | glpF | b3927 | 0.41 | +0.2 |
4,115,632 | - | glpF | b3927 | 0.43 | -1.3 |
4,115,632 | - | glpF | b3927 | 0.43 | -0.0 |
4,115,634 | + | glpF | b3927 | 0.43 | +0.1 |
4,115,642 | - | glpF | b3927 | 0.44 | -1.3 |
4,115,647 | - | glpF | b3927 | 0.45 | +0.1 |
4,115,657 | + | glpF | b3927 | 0.46 | +0.3 |
4,115,692 | + | glpF | b3927 | 0.50 | -0.8 |
4,115,723 | + | glpF | b3927 | 0.54 | +0.6 |
4,115,742 | + | glpF | b3927 | 0.56 | +0.2 |
4,115,742 | + | glpF | b3927 | 0.56 | -0.6 |
4,115,761 | - | glpF | b3927 | 0.58 | -0.0 |
4,115,793 | - | glpF | b3927 | 0.62 | +0.4 |
4,115,793 | - | glpF | b3927 | 0.62 | +0.6 |
4,115,865 | - | glpF | b3927 | 0.71 | +0.6 |
4,115,865 | - | glpF | b3927 | 0.71 | -0.3 |
4,115,886 | + | glpF | b3927 | 0.73 | +1.0 |
4,115,889 | + | glpF | b3927 | 0.73 | -0.4 |
4,115,893 | - | glpF | b3927 | 0.74 | +2.0 |
4,115,953 | + | glpF | b3927 | 0.81 | -0.3 |
4,115,956 | + | glpF | b3927 | 0.81 | -0.0 |
4,115,956 | + | glpF | b3927 | 0.81 | +1.9 |
4,115,960 | + | glpF | b3927 | 0.82 | -0.0 |
4,115,960 | + | glpF | b3927 | 0.82 | +0.1 |
4,115,964 | - | glpF | b3927 | 0.82 | +0.4 |
4,115,966 | + | glpF | b3927 | 0.83 | +0.4 |
4,115,968 | - | glpF | b3927 | 0.83 | +2.2 |
4,116,014 | + | glpF | b3927 | 0.88 | -0.7 |
4,116,023 | + | glpF | b3927 | 0.89 | +1.2 |
4,116,023 | + | glpF | b3927 | 0.89 | +0.0 |
4,116,027 | + | glpF | b3927 | 0.90 | -0.7 |
4,116,036 | - | +0.0 | |||
4,116,056 | + | -1.0 | |||
4,116,081 | + | -0.8 | |||
4,116,101 | + | -0.8 | |||
4,116,101 | + | +0.0 | |||
4,116,103 | + | +1.2 | |||
4,116,138 | - | -0.6 | |||
4,116,148 | + | -0.3 | |||
4,116,171 | + | +0.6 | |||
4,116,174 | + | +0.6 | |||
4,116,175 | + | -0.1 | |||
4,116,176 | + | -0.4 | |||
4,116,193 | - | +0.5 | |||
4,116,236 | + | +0.4 | |||
4,116,236 | + | -0.5 | |||
4,116,236 | - | -1.7 | |||
4,116,236 | - | +2.5 | |||
4,116,241 | - | +0.6 | |||
4,116,241 | - | -0.8 | |||
4,116,289 | + | -1.5 | |||
4,116,289 | + | -0.5 | |||
4,116,292 | + | +0.2 | |||
4,116,293 | - | -0.0 | |||
4,116,355 | - | +0.2 | |||
4,116,360 | - | +0.4 | |||
4,116,381 | + | -0.6 | |||
4,116,398 | + | +0.2 | |||
4,116,415 | + | +0.0 | |||
4,116,415 | + | -0.1 | |||
4,116,415 | + | +0.0 | |||
4,116,418 | - | +0.9 | |||
4,116,420 | + | +0.2 | |||
4,116,459 | - | -0.1 | |||
4,116,528 | - | +0.3 | |||
4,116,533 | - | -0.2 | |||
4,116,534 | + | +0.4 | |||
4,116,535 | + | -0.1 | |||
4,116,542 | - | -0.6 | |||
4,116,543 | - | -1.0 | |||
4,116,543 | - | +0.0 | |||
4,116,548 | + | +0.4 | |||
4,116,548 | + | -1.1 | |||
4,116,548 | + | -0.5 | |||
4,116,564 | - | yiiU | b3928 | 0.11 | -1.3 |
4,116,580 | + | yiiU | b3928 | 0.17 | -0.2 |
4,116,592 | + | yiiU | b3928 | 0.22 | -0.3 |
4,116,597 | + | yiiU | b3928 | 0.24 | +0.5 |
4,116,599 | + | yiiU | b3928 | 0.25 | -0.1 |
4,116,599 | + | yiiU | b3928 | 0.25 | +0.8 |
4,116,603 | - | yiiU | b3928 | 0.26 | -0.2 |
4,116,607 | + | yiiU | b3928 | 0.28 | -0.6 |
4,116,647 | + | yiiU | b3928 | 0.44 | +1.0 |
4,116,647 | + | yiiU | b3928 | 0.44 | +1.4 |
4,116,654 | + | yiiU | b3928 | 0.47 | -0.5 |
4,116,683 | + | yiiU | b3928 | 0.59 | -0.1 |
4,116,686 | - | yiiU | b3928 | 0.60 | +1.0 |
4,116,686 | - | yiiU | b3928 | 0.60 | +1.0 |
4,116,691 | + | yiiU | b3928 | 0.62 | +1.2 |
4,116,691 | + | yiiU | b3928 | 0.62 | +1.6 |
4,116,748 | + | yiiU | b3928 | 0.85 | -0.3 |
4,116,767 | + | +0.3 | |||
4,116,767 | + | +0.3 | |||
4,116,767 | + | -0.4 | |||
4,116,775 | - | +0.5 | |||
4,116,782 | + | -0.2 | |||
4,116,782 | + | +0.2 | |||
4,116,785 | - | +1.3 | |||
4,116,789 | - | -0.2 | |||
4,116,789 | - | -1.0 | |||
4,116,816 | + | +0.4 | |||
4,116,816 | + | +0.3 | |||
4,116,816 | + | +0.4 | |||
4,116,824 | - | +0.5 | |||
4,116,824 | - | +0.3 | |||
4,116,824 | - | +0.9 | |||
4,116,824 | - | -0.8 | |||
4,116,824 | - | -2.0 | |||
4,116,858 | + | -0.1 | |||
4,116,883 | - | +0.5 | |||
4,116,883 | - | -0.2 | |||
4,116,930 | + | rraA | b3929 | 0.13 | -0.9 |
4,116,930 | + | rraA | b3929 | 0.13 | -0.7 |
4,116,932 | - | rraA | b3929 | 0.13 | +0.3 |
4,116,932 | - | rraA | b3929 | 0.13 | -1.3 |
4,116,940 | + | rraA | b3929 | 0.15 | -0.2 |
4,116,960 | - | rraA | b3929 | 0.19 | -0.8 |
4,117,029 | - | rraA | b3929 | 0.33 | +0.6 |
4,117,029 | - | rraA | b3929 | 0.33 | -1.5 |
4,117,039 | - | rraA | b3929 | 0.35 | +0.0 |
4,117,050 | - | rraA | b3929 | 0.37 | +0.1 |
4,117,083 | - | rraA | b3929 | 0.44 | -0.9 |
4,117,083 | - | rraA | b3929 | 0.44 | -1.0 |
Or see this region's nucleotide sequence