Strain Fitness in Escherichia coli BW25113 around b2389

Experiment: LB Anaerobic with Spectinomycin 0.0000625 mM

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	LB Anaerobic with Spectinomycin 0.0000625 mM
2,506,731	-	glk	b2388	0.26	+1.1
2,506,731	-	glk	b2388	0.26	+1.0
2,506,780	-	glk	b2388	0.31	-0.9
2,506,800	-	glk	b2388	0.33	-0.0
2,506,861	-	glk	b2388	0.39	+0.4
2,506,866	+	glk	b2388	0.40	+0.2
2,507,004	+	glk	b2388	0.54	+1.7
2,507,004	+	glk	b2388	0.54	-0.9
2,507,022	+	glk	b2388	0.56	+0.4
2,507,057	+	glk	b2388	0.59	+0.4
2,507,185	+	glk	b2388	0.73	-0.2
2,507,215	+	glk	b2388	0.76	-1.6
2,507,333	+	glk	b2388	0.88	-0.7
2,507,341	-	glk	b2388	0.89	+0.9
2,507,341	-	glk	b2388	0.89	+0.7
2,507,362	-				+0.8
2,507,362	-				+0.1
2,507,362	-				+1.0
2,507,392	-				-0.3
2,507,407	-				-0.5
2,507,448	+				+0.2
2,507,483	+				-0.3
2,507,493	+				+0.6
2,507,499	-				+0.8
2,507,506	-				-0.4
2,507,513	-				+0.3
2,507,540	+				-0.4
2,507,634	-				-1.3
2,507,634	-				-0.0
2,507,634	-				+0.2
2,507,803	-	yfeO	b2389	0.12	-0.7
2,507,805	+	yfeO	b2389	0.12	-0.4
2,507,835	+	yfeO	b2389	0.15	-0.3
2,507,883	-	yfeO	b2389	0.18	-1.0
2,507,883	-	yfeO	b2389	0.18	-0.3
2,507,901	+	yfeO	b2389	0.20	-0.5
2,507,904	-	yfeO	b2389	0.20	-0.5
2,508,013	+	yfeO	b2389	0.29	+1.3
2,508,302	+	yfeO	b2389	0.52	+0.1
2,508,328	+	yfeO	b2389	0.54	-0.3
2,508,368	-	yfeO	b2389	0.57	+0.1
2,508,377	+	yfeO	b2389	0.58	+1.4
2,508,377	+	yfeO	b2389	0.58	-0.1
2,508,377	+	yfeO	b2389	0.58	-0.5
2,508,457	+	yfeO	b2389	0.64	-0.6
2,508,457	+	yfeO	b2389	0.64	-1.5
2,508,465	-	yfeO	b2389	0.65	-1.1
2,508,518	-	yfeO	b2389	0.69	-1.0
2,508,537	+	yfeO	b2389	0.70	-0.6
2,508,537	+	yfeO	b2389	0.70	-1.2
2,508,737	-	yfeO	b2389	0.86	-0.4
2,508,780	+	yfeO	b2389	0.90	-0.7
2,508,791	-				-0.6
2,508,793	-				+0.4
2,508,793	-				+0.1
2,508,799	+				+0.2
2,508,812	+				-0.9
2,508,812	+				+0.6
2,508,814	+				-1.3
2,508,817	-				+0.8
2,508,844	-				-0.4
2,508,950	+				-1.9
2,508,964	-				+1.1
2,509,096	+	ypeC	b2390	0.22	+0.5
2,509,156	-	ypeC	b2390	0.41	+0.6
2,509,174	+	ypeC	b2390	0.46	+0.3
2,509,182	-	ypeC	b2390	0.49	+0.2
2,509,182	-	ypeC	b2390	0.49	+1.2
2,509,251	-	ypeC	b2390	0.70	-1.4
2,509,251	-				+1.3
2,509,338	-				+0.4
2,509,338	-				-0.8
2,509,375	-				+0.2
2,509,403	+				+0.1
2,509,427	-				-0.2
2,509,553	+				-0.9
2,509,581	+				+0.9
2,509,704	-	mntH	b2392	0.17	-0.2
2,509,711	-	mntH	b2392	0.18	+0.3
2,509,711	-	mntH	b2392	0.18	+0.3
2,509,772	-	mntH	b2392	0.23	+0.3
2,509,809	+	mntH	b2392	0.26	+0.2
2,509,811	+	mntH	b2392	0.26	+1.3
2,509,839	-	mntH	b2392	0.28	-0.0
2,509,875	-	mntH	b2392	0.31	+0.3