Experiment: LB Anaerobic with Spectinomycin 0.0000625 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt deoR and ybjG are separated by 57 nucleotides ybjG and cmr are separated by 284 nucleotides cmr and ybjH are separated by 40 nucleotides ybjH and b0844 are separated by 85 nucleotides
b0840: deoR - DNA-binding transcriptional repressor (NCBI), at 881,199 to 881,957
deoR
b0841: ybjG - undecaprenyl pyrophosphate phosphatase (NCBI), at 882,015 to 882,611
ybjG
b0842: cmr - multidrug efflux system protein (NCBI), at 882,896 to 884,128
cmr
b0843: ybjH - hypothetical protein (NCBI), at 884,169 to 884,453
ybjH
b0844: b0844 - orf, hypothetical protein (VIMSS), at 884,539 to 885,354
b0844
Position (kb) 882
883
884
885 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5
6
7 at 881.902 kb on - strand at 881.906 kb on + strand at 881.906 kb on + strand at 881.957 kb on - strand at 881.957 kb on - strand at 882.047 kb on + strand at 882.231 kb on + strand, within ybjG at 882.251 kb on - strand, within ybjG at 882.298 kb on - strand, within ybjG at 882.418 kb on - strand, within ybjG at 882.455 kb on + strand, within ybjG at 882.485 kb on - strand, within ybjG at 882.485 kb on - strand, within ybjG at 882.551 kb on - strand, within ybjG at 882.589 kb on - strand at 882.645 kb on + strand at 882.664 kb on - strand at 882.671 kb on + strand at 882.695 kb on + strand at 882.719 kb on - strand at 882.723 kb on + strand at 882.731 kb on - strand at 882.738 kb on + strand at 882.745 kb on - strand at 882.746 kb on + strand at 882.746 kb on + strand at 882.785 kb on - strand at 882.802 kb on - strand at 882.804 kb on + strand at 882.819 kb on - strand at 882.819 kb on - strand at 882.819 kb on - strand at 882.918 kb on + strand at 883.008 kb on + strand at 883.016 kb on - strand at 883.016 kb on - strand at 883.018 kb on + strand at 883.033 kb on + strand, within cmr at 883.033 kb on + strand, within cmr at 883.059 kb on + strand, within cmr at 883.061 kb on + strand, within cmr at 883.063 kb on - strand, within cmr at 883.097 kb on + strand, within cmr at 883.338 kb on - strand, within cmr at 883.528 kb on + strand, within cmr at 883.528 kb on + strand, within cmr at 883.528 kb on + strand, within cmr at 883.594 kb on + strand, within cmr at 883.725 kb on - strand, within cmr at 883.725 kb on - strand, within cmr at 883.777 kb on + strand, within cmr at 883.798 kb on - strand, within cmr at 883.798 kb on - strand, within cmr at 883.838 kb on - strand, within cmr at 883.872 kb on + strand, within cmr at 883.872 kb on + strand, within cmr at 883.927 kb on + strand, within cmr at 883.961 kb on + strand, within cmr at 883.961 kb on + strand, within cmr at 884.023 kb on - strand at 884.041 kb on + strand at 884.041 kb on + strand at 884.095 kb on + strand at 884.146 kb on - strand at 884.299 kb on + strand, within ybjH at 884.355 kb on + strand, within ybjH at 884.355 kb on + strand, within ybjH at 884.502 kb on + strand at 884.523 kb on - strand at 884.553 kb on - strand at 884.594 kb on + strand at 884.613 kb on - strand at 884.613 kb on - strand at 884.674 kb on + strand, within b0844 at 884.692 kb on + strand, within b0844 at 884.713 kb on + strand, within b0844 at 884.789 kb on - strand, within b0844 at 884.807 kb on + strand, within b0844 at 884.815 kb on - strand, within b0844 at 884.833 kb on + strand, within b0844 at 884.835 kb on + strand, within b0844 at 884.956 kb on - strand, within b0844 at 884.956 kb on - strand, within b0844 at 885.073 kb on + strand, within b0844 at 885.095 kb on + strand, within b0844 at 885.095 kb on + strand, within b0844 at 885.095 kb on + strand, within b0844 at 885.105 kb on - strand, within b0844
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Spectinomycin 0.0000625 mM remove 881,902 - +1.6 881,906 + +0.6 881,906 + +1.9 881,957 - +1.9 881,957 - +1.5 882,047 + +0.9 882,231 + ybjG b0841 0.36 -0.3 882,251 - ybjG b0841 0.40 +0.9 882,298 - ybjG b0841 0.47 +0.9 882,418 - ybjG b0841 0.68 +2.2 882,455 + ybjG b0841 0.74 -1.4 882,485 - ybjG b0841 0.79 +3.1 882,485 - ybjG b0841 0.79 +2.8 882,551 - ybjG b0841 0.90 +3.8 882,589 - +4.3 882,645 + -2.6 882,664 - +3.7 882,671 + -3.0 882,695 + -3.0 882,719 - +1.2 882,723 + -2.4 882,731 - +1.3 882,738 + -2.4 882,745 - +3.1 882,746 + +0.2 882,746 + +0.2 882,785 - +1.5 882,802 - -0.4 882,804 + -1.1 882,819 - +3.6 882,819 - +2.1 882,819 - +2.8 882,918 + +5.7 883,008 + +5.5 883,016 - +3.2 883,016 - +5.2 883,018 + +5.9 883,033 + cmr b0842 0.11 +6.6 883,033 + cmr b0842 0.11 +6.5 883,059 + cmr b0842 0.13 +5.4 883,061 + cmr b0842 0.13 +5.8 883,063 - cmr b0842 0.14 +5.2 883,097 + cmr b0842 0.16 +5.7 883,338 - cmr b0842 0.36 +4.3 883,528 + cmr b0842 0.51 +6.2 883,528 + cmr b0842 0.51 +5.3 883,528 + cmr b0842 0.51 +5.8 883,594 + cmr b0842 0.57 +5.1 883,725 - cmr b0842 0.67 +4.6 883,725 - cmr b0842 0.67 +6.2 883,777 + cmr b0842 0.71 +5.6 883,798 - cmr b0842 0.73 +5.7 883,798 - cmr b0842 0.73 +7.1 883,838 - cmr b0842 0.76 +5.7 883,872 + cmr b0842 0.79 +6.1 883,872 + cmr b0842 0.79 +6.4 883,927 + cmr b0842 0.84 +6.5 883,961 + cmr b0842 0.86 +6.3 883,961 + cmr b0842 0.86 +6.4 884,023 - +5.7 884,041 + +5.0 884,041 + +5.3 884,095 + +6.0 884,146 - +4.6 884,299 + ybjH b0843 0.46 +0.2 884,355 + ybjH b0843 0.65 +1.0 884,355 + ybjH b0843 0.65 +0.6 884,502 + +1.7 884,523 - +0.6 884,553 - +0.9 884,594 + +0.2 884,613 - +1.1 884,613 - +0.8 884,674 + b0844 b0844 0.17 +0.6 884,692 + b0844 b0844 0.19 -1.0 884,713 + b0844 b0844 0.21 -0.4 884,789 - b0844 b0844 0.31 +1.1 884,807 + b0844 b0844 0.33 -0.2 884,815 - b0844 b0844 0.34 +0.7 884,833 + b0844 b0844 0.36 +1.6 884,835 + b0844 b0844 0.36 -1.0 884,956 - b0844 b0844 0.51 +1.3 884,956 - b0844 b0844 0.51 -1.7 885,073 + b0844 b0844 0.65 +0.6 885,095 + b0844 b0844 0.68 -1.0 885,095 + b0844 b0844 0.68 -0.4 885,095 + b0844 b0844 0.68 +0.6 885,105 - b0844 b0844 0.69 +1.0
Or see this region's nucleotide sequence