Experiment: LB Anaerobic with Doxycycline hyclate 0.000000390625 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hybB and hybA overlap by 11 nucleotides hybA and hybO are separated by 2 nucleotides
b2995: hybB - predicted hydrogenase 2 cytochrome b type component (NCBI), at 3,141,008 to 3,142,186
hybB
b2996: hybA - hydrogenase 2 4Fe-4S ferredoxin-type component (NCBI), at 3,142,176 to 3,143,162
hybA
b2997: hybO - hydrogenase 2, small subunit (NCBI), at 3,143,165 to 3,144,283
hybO
Position (kb)
3142
3143
3144 Strain fitness (log2 ratio)
-2
-1
0
1 at 3141.192 kb on - strand, within hybB at 3141.215 kb on + strand, within hybB at 3141.215 kb on + strand, within hybB at 3141.223 kb on - strand, within hybB at 3141.240 kb on - strand, within hybB at 3141.240 kb on - strand, within hybB at 3141.286 kb on - strand, within hybB at 3141.341 kb on - strand, within hybB at 3141.341 kb on - strand, within hybB at 3141.472 kb on + strand, within hybB at 3141.486 kb on + strand, within hybB at 3141.554 kb on - strand, within hybB at 3141.632 kb on + strand, within hybB at 3141.642 kb on + strand, within hybB at 3141.642 kb on + strand, within hybB at 3141.657 kb on - strand, within hybB at 3141.676 kb on - strand, within hybB at 3141.733 kb on - strand, within hybB at 3141.738 kb on - strand, within hybB at 3141.738 kb on - strand, within hybB at 3141.751 kb on - strand, within hybB at 3141.751 kb on - strand, within hybB at 3141.779 kb on - strand, within hybB at 3141.804 kb on - strand, within hybB at 3141.808 kb on + strand, within hybB at 3141.843 kb on + strand, within hybB at 3141.851 kb on - strand, within hybB at 3141.888 kb on + strand, within hybB at 3141.901 kb on - strand, within hybB at 3141.901 kb on - strand, within hybB at 3141.913 kb on - strand, within hybB at 3141.955 kb on - strand, within hybB at 3141.955 kb on - strand, within hybB at 3142.110 kb on - strand at 3142.110 kb on - strand at 3142.271 kb on - strand at 3142.284 kb on - strand, within hybA at 3142.284 kb on - strand, within hybA at 3142.284 kb on - strand, within hybA at 3142.286 kb on - strand, within hybA at 3142.286 kb on - strand, within hybA at 3142.368 kb on + strand, within hybA at 3142.410 kb on + strand, within hybA at 3142.446 kb on - strand, within hybA at 3142.585 kb on - strand, within hybA at 3142.585 kb on - strand, within hybA at 3142.587 kb on + strand, within hybA at 3142.600 kb on + strand, within hybA at 3142.676 kb on - strand, within hybA at 3142.719 kb on - strand, within hybA at 3142.719 kb on - strand, within hybA at 3142.865 kb on + strand, within hybA at 3142.877 kb on + strand, within hybA at 3142.885 kb on - strand, within hybA at 3142.995 kb on - strand, within hybA at 3142.995 kb on - strand, within hybA at 3143.008 kb on + strand, within hybA at 3143.108 kb on - strand at 3143.108 kb on - strand at 3143.127 kb on + strand at 3143.127 kb on + strand at 3143.223 kb on + strand at 3143.230 kb on + strand at 3143.230 kb on + strand at 3143.277 kb on - strand, within hybO at 3143.279 kb on - strand, within hybO at 3143.410 kb on - strand, within hybO at 3143.410 kb on - strand, within hybO at 3143.456 kb on + strand, within hybO at 3143.471 kb on - strand, within hybO at 3143.475 kb on + strand, within hybO at 3143.499 kb on - strand, within hybO at 3143.515 kb on - strand, within hybO at 3143.521 kb on - strand, within hybO at 3143.656 kb on + strand, within hybO at 3143.677 kb on - strand at 3143.677 kb on - strand, within hybO at 3143.799 kb on - strand, within hybO at 3143.861 kb on - strand, within hybO at 3143.897 kb on + strand, within hybO at 3143.960 kb on - strand, within hybO at 3143.995 kb on + strand, within hybO at 3144.007 kb on + strand, within hybO at 3144.091 kb on + strand, within hybO at 3144.105 kb on + strand, within hybO at 3144.105 kb on + strand, within hybO
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Doxycycline hyclate 0.000000390625 mM remove 3,141,192 - hybB b2995 0.16 +0.5 3,141,215 + hybB b2995 0.18 +1.3 3,141,215 + hybB b2995 0.18 -1.4 3,141,223 - hybB b2995 0.18 -0.3 3,141,240 - hybB b2995 0.20 -1.4 3,141,240 - hybB b2995 0.20 -0.4 3,141,286 - hybB b2995 0.24 -0.5 3,141,341 - hybB b2995 0.28 -0.5 3,141,341 - hybB b2995 0.28 -1.8 3,141,472 + hybB b2995 0.39 +0.0 3,141,486 + hybB b2995 0.41 +0.5 3,141,554 - hybB b2995 0.46 +1.1 3,141,632 + hybB b2995 0.53 +0.8 3,141,642 + hybB b2995 0.54 +0.1 3,141,642 + hybB b2995 0.54 -0.4 3,141,657 - hybB b2995 0.55 -0.4 3,141,676 - hybB b2995 0.57 -1.6 3,141,733 - hybB b2995 0.61 -0.0 3,141,738 - hybB b2995 0.62 -0.8 3,141,738 - hybB b2995 0.62 -1.5 3,141,751 - hybB b2995 0.63 -0.8 3,141,751 - hybB b2995 0.63 -1.1 3,141,779 - hybB b2995 0.65 -1.0 3,141,804 - hybB b2995 0.68 -0.2 3,141,808 + hybB b2995 0.68 -0.3 3,141,843 + hybB b2995 0.71 -0.1 3,141,851 - hybB b2995 0.72 -0.3 3,141,888 + hybB b2995 0.75 -0.4 3,141,901 - hybB b2995 0.76 +0.4 3,141,901 - hybB b2995 0.76 -0.9 3,141,913 - hybB b2995 0.77 -0.2 3,141,955 - hybB b2995 0.80 -0.2 3,141,955 - hybB b2995 0.80 -0.2 3,142,110 - -0.8 3,142,110 - +0.1 3,142,271 - -0.8 3,142,284 - hybA b2996 0.11 -0.1 3,142,284 - hybA b2996 0.11 -1.4 3,142,284 - hybA b2996 0.11 +0.5 3,142,286 - hybA b2996 0.11 -0.8 3,142,286 - hybA b2996 0.11 -0.2 3,142,368 + hybA b2996 0.19 -1.8 3,142,410 + hybA b2996 0.24 +0.6 3,142,446 - hybA b2996 0.27 -0.9 3,142,585 - hybA b2996 0.41 -2.0 3,142,585 - hybA b2996 0.41 -0.4 3,142,587 + hybA b2996 0.42 -1.0 3,142,600 + hybA b2996 0.43 -0.6 3,142,676 - hybA b2996 0.51 -0.3 3,142,719 - hybA b2996 0.55 -0.6 3,142,719 - hybA b2996 0.55 -1.0 3,142,865 + hybA b2996 0.70 -0.5 3,142,877 + hybA b2996 0.71 -0.2 3,142,885 - hybA b2996 0.72 -1.3 3,142,995 - hybA b2996 0.83 -1.1 3,142,995 - hybA b2996 0.83 -0.8 3,143,008 + hybA b2996 0.84 -1.3 3,143,108 - +0.2 3,143,108 - +0.1 3,143,127 + -0.5 3,143,127 + -1.1 3,143,223 + -0.8 3,143,230 + -0.2 3,143,230 + -0.8 3,143,277 - hybO b2997 0.10 -0.7 3,143,279 - hybO b2997 0.10 -1.6 3,143,410 - hybO b2997 0.22 -1.1 3,143,410 - hybO b2997 0.22 -0.0 3,143,456 + hybO b2997 0.26 -0.2 3,143,471 - hybO b2997 0.27 -1.5 3,143,475 + hybO b2997 0.28 -0.3 3,143,499 - hybO b2997 0.30 -1.4 3,143,515 - hybO b2997 0.31 -0.4 3,143,521 - hybO b2997 0.32 -1.1 3,143,656 + hybO b2997 0.44 -0.5 3,143,677 - -1.2 3,143,677 - hybO b2997 0.46 +1.2 3,143,799 - hybO b2997 0.57 -1.1 3,143,861 - hybO b2997 0.62 -0.2 3,143,897 + hybO b2997 0.65 -0.4 3,143,960 - hybO b2997 0.71 -0.2 3,143,995 + hybO b2997 0.74 -0.7 3,144,007 + hybO b2997 0.75 -0.8 3,144,091 + hybO b2997 0.83 -1.0 3,144,105 + hybO b2997 0.84 -0.1 3,144,105 + hybO b2997 0.84 -1.1
Or see this region's nucleotide sequence