Experiment: LB Anaerobic with Doxycycline hyclate 0.000000390625 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt amiA and hemF are separated by 3 nucleotides hemF and yfeG are separated by 5 nucleotides yfeG and b2438 are separated by 45 nucleotides b2438 and eutL are separated by 12 nucleotides
b2435: amiA - N-acetylmuramoyl-l-alanine amidase I (NCBI), at 2,550,374 to 2,551,243
amiA
b2436: hemF - coproporphyrinogen III oxidase (NCBI), at 2,551,247 to 2,552,146
hemF
b2437: yfeG - predicted DNA-binding transcriptional regulator (NCBI), at 2,552,152 to 2,553,204
yfeG
b2438: b2438 - orf, hypothetical protein (VIMSS), at 2,553,250 to 2,553,750
b2438
b2439: eutL - predicted carboxysome structural protein with predicted role in ethanolamine utilization (NCBI), at 2,553,763 to 2,554,422
eutL
Position (kb)
2552
2553
2554 Strain fitness (log2 ratio)
-2
-1
0
1 at 2551.197 kb on + strand at 2551.266 kb on + strand at 2551.295 kb on - strand at 2551.295 kb on - strand at 2551.355 kb on - strand, within hemF at 2551.444 kb on + strand, within hemF at 2551.444 kb on + strand, within hemF at 2551.531 kb on - strand, within hemF at 2551.643 kb on - strand, within hemF at 2551.645 kb on + strand, within hemF at 2551.678 kb on + strand, within hemF at 2551.678 kb on + strand, within hemF at 2551.837 kb on + strand, within hemF at 2551.837 kb on + strand, within hemF at 2552.065 kb on - strand at 2552.065 kb on - strand at 2552.087 kb on + strand at 2552.087 kb on + strand at 2552.141 kb on + strand at 2552.262 kb on - strand, within yfeG at 2552.262 kb on - strand, within yfeG at 2552.460 kb on + strand, within yfeG at 2552.460 kb on + strand, within yfeG at 2552.659 kb on - strand, within yfeG at 2552.659 kb on - strand, within yfeG at 2552.719 kb on - strand, within yfeG at 2552.790 kb on - strand, within yfeG at 2552.790 kb on - strand, within yfeG at 2552.812 kb on - strand, within yfeG at 2552.812 kb on - strand, within yfeG at 2552.881 kb on + strand, within yfeG at 2552.881 kb on + strand, within yfeG at 2552.917 kb on + strand, within yfeG at 2552.917 kb on + strand, within yfeG at 2552.932 kb on + strand, within yfeG at 2552.992 kb on - strand, within yfeG at 2553.227 kb on + strand at 2553.256 kb on - strand at 2553.292 kb on + strand at 2553.469 kb on - strand, within b2438 at 2553.469 kb on - strand, within b2438 at 2553.477 kb on + strand, within b2438 at 2553.478 kb on + strand, within b2438 at 2553.478 kb on + strand, within b2438 at 2553.478 kb on + strand, within b2438 at 2553.478 kb on + strand, within b2438 at 2553.513 kb on - strand, within b2438 at 2553.554 kb on - strand, within b2438 at 2553.598 kb on - strand, within b2438 at 2553.732 kb on + strand at 2553.922 kb on + strand, within eutL at 2554.133 kb on - strand, within eutL at 2554.133 kb on - strand, within eutL
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Doxycycline hyclate 0.000000390625 mM remove 2,551,197 + -0.2 2,551,266 + +0.1 2,551,295 - -0.8 2,551,295 - +1.3 2,551,355 - hemF b2436 0.12 -0.6 2,551,444 + hemF b2436 0.22 -1.4 2,551,444 + hemF b2436 0.22 +1.6 2,551,531 - hemF b2436 0.32 +0.5 2,551,643 - hemF b2436 0.44 -0.1 2,551,645 + hemF b2436 0.44 +0.3 2,551,678 + hemF b2436 0.48 -0.1 2,551,678 + hemF b2436 0.48 +0.9 2,551,837 + hemF b2436 0.66 -0.3 2,551,837 + hemF b2436 0.66 +0.0 2,552,065 - -0.2 2,552,065 - -2.0 2,552,087 + +0.3 2,552,087 + +0.3 2,552,141 + -0.2 2,552,262 - yfeG b2437 0.10 +0.0 2,552,262 - yfeG b2437 0.10 -0.5 2,552,460 + yfeG b2437 0.29 -1.2 2,552,460 + yfeG b2437 0.29 -0.5 2,552,659 - yfeG b2437 0.48 -2.0 2,552,659 - yfeG b2437 0.48 -0.4 2,552,719 - yfeG b2437 0.54 -0.8 2,552,790 - yfeG b2437 0.61 -1.2 2,552,790 - yfeG b2437 0.61 +0.4 2,552,812 - yfeG b2437 0.63 -0.6 2,552,812 - yfeG b2437 0.63 -0.1 2,552,881 + yfeG b2437 0.69 +0.4 2,552,881 + yfeG b2437 0.69 +0.3 2,552,917 + yfeG b2437 0.73 -0.5 2,552,917 + yfeG b2437 0.73 -0.5 2,552,932 + yfeG b2437 0.74 -2.4 2,552,992 - yfeG b2437 0.80 -1.0 2,553,227 + +0.2 2,553,256 - +0.9 2,553,292 + -0.4 2,553,469 - b2438 b2438 0.44 -0.6 2,553,469 - b2438 b2438 0.44 -0.6 2,553,477 + b2438 b2438 0.45 -0.6 2,553,478 + b2438 b2438 0.46 +0.2 2,553,478 + b2438 b2438 0.46 +0.6 2,553,478 + b2438 b2438 0.46 -1.1 2,553,478 + b2438 b2438 0.46 +0.9 2,553,513 - b2438 b2438 0.52 -0.8 2,553,554 - b2438 b2438 0.61 -0.4 2,553,598 - b2438 b2438 0.69 +0.0 2,553,732 + -1.1 2,553,922 + eutL b2439 0.24 -0.2 2,554,133 - eutL b2439 0.56 +0.8 2,554,133 - eutL b2439 0.56 -0.3
Or see this region's nucleotide sequence