Experiment: LB Anaerobic with Doxycycline hyclate 0.000000390625 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ynbD and azoR are separated by 53 nucleotides azoR and hrpA are separated by 200 nucleotides hrpA and ydcF are separated by 271 nucleotides
b1411: ynbD - predicted phosphatase, inner membrane protein (NCBI), at 1,478,933 to 1,480,225
ynbD
b1412: azoR - acyl carrier protein phosphodiesterase (NCBI), at 1,480,279 to 1,480,884
azoR
b1413: hrpA - ATP-dependent helicase (RefSeq), at 1,481,085 to 1,484,987
hrpA
b1414: ydcF - hypothetical protein (NCBI), at 1,485,259 to 1,486,059
ydcF
Position (kb)
1481
1482
1483
1484
1485 Strain fitness (log2 ratio)
-2
-1
0
1 at 1480.154 kb on - strand at 1480.167 kb on + strand at 1480.271 kb on + strand at 1480.271 kb on + strand at 1480.667 kb on - strand, within azoR at 1480.729 kb on + strand, within azoR at 1480.729 kb on + strand, within azoR at 1481.461 kb on + strand at 1481.461 kb on + strand at 1481.608 kb on + strand, within hrpA at 1481.635 kb on - strand, within hrpA at 1481.646 kb on + strand, within hrpA at 1481.646 kb on + strand, within hrpA at 1481.804 kb on + strand, within hrpA at 1481.804 kb on + strand, within hrpA at 1481.916 kb on + strand, within hrpA at 1481.956 kb on + strand, within hrpA at 1481.956 kb on + strand, within hrpA at 1482.025 kb on + strand, within hrpA at 1482.062 kb on + strand, within hrpA at 1482.181 kb on + strand, within hrpA at 1482.187 kb on - strand, within hrpA at 1482.187 kb on - strand, within hrpA at 1482.187 kb on - strand, within hrpA at 1482.187 kb on - strand, within hrpA at 1482.314 kb on + strand, within hrpA at 1482.314 kb on + strand, within hrpA at 1482.417 kb on - strand, within hrpA at 1482.553 kb on + strand, within hrpA at 1483.054 kb on + strand, within hrpA at 1483.877 kb on + strand, within hrpA at 1483.921 kb on + strand, within hrpA at 1484.433 kb on + strand, within hrpA at 1484.566 kb on - strand, within hrpA at 1484.615 kb on - strand at 1484.883 kb on + strand at 1484.883 kb on + strand at 1485.446 kb on + strand, within ydcF at 1485.502 kb on + strand, within ydcF at 1485.553 kb on - strand, within ydcF at 1485.833 kb on + strand, within ydcF at 1485.869 kb on - strand, within ydcF at 1485.881 kb on + strand, within ydcF at 1485.881 kb on + strand, within ydcF at 1485.887 kb on - strand, within ydcF at 1485.899 kb on - strand, within ydcF at 1485.899 kb on - strand, within ydcF
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Doxycycline hyclate 0.000000390625 mM remove 1,480,154 - -1.2 1,480,167 + +0.4 1,480,271 + +0.4 1,480,271 + +0.5 1,480,667 - azoR b1412 0.64 +0.4 1,480,729 + azoR b1412 0.74 -0.3 1,480,729 + azoR b1412 0.74 +1.0 1,481,461 + -1.6 1,481,461 + -1.0 1,481,608 + hrpA b1413 0.13 -0.7 1,481,635 - hrpA b1413 0.14 +0.8 1,481,646 + hrpA b1413 0.14 -0.9 1,481,646 + hrpA b1413 0.14 -0.3 1,481,804 + hrpA b1413 0.18 +0.1 1,481,804 + hrpA b1413 0.18 -0.6 1,481,916 + hrpA b1413 0.21 -0.4 1,481,956 + hrpA b1413 0.22 -1.3 1,481,956 + hrpA b1413 0.22 -1.0 1,482,025 + hrpA b1413 0.24 -0.2 1,482,062 + hrpA b1413 0.25 -1.3 1,482,181 + hrpA b1413 0.28 -0.2 1,482,187 - hrpA b1413 0.28 -1.0 1,482,187 - hrpA b1413 0.28 -0.1 1,482,187 - hrpA b1413 0.28 -0.2 1,482,187 - hrpA b1413 0.28 -2.1 1,482,314 + hrpA b1413 0.31 +0.9 1,482,314 + hrpA b1413 0.31 -1.6 1,482,417 - hrpA b1413 0.34 +0.0 1,482,553 + hrpA b1413 0.38 -1.2 1,483,054 + hrpA b1413 0.50 -2.5 1,483,877 + hrpA b1413 0.72 -0.9 1,483,921 + hrpA b1413 0.73 -0.2 1,484,433 + hrpA b1413 0.86 -1.1 1,484,566 - hrpA b1413 0.89 -1.6 1,484,615 - -1.4 1,484,883 + -1.0 1,484,883 + -1.0 1,485,446 + ydcF b1414 0.23 -1.0 1,485,502 + ydcF b1414 0.30 +0.1 1,485,553 - ydcF b1414 0.37 +0.5 1,485,833 + ydcF b1414 0.72 -0.3 1,485,869 - ydcF b1414 0.76 +0.2 1,485,881 + ydcF b1414 0.78 +0.2 1,485,881 + ydcF b1414 0.78 -0.6 1,485,887 - ydcF b1414 0.78 +0.7 1,485,899 - ydcF b1414 0.80 -0.6 1,485,899 - ydcF b1414 0.80 +0.7
Or see this region's nucleotide sequence