Experiment: LB 0.1x with 55.5 mM D-Glucose; pH 4.6
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_4014 and PP_4015 overlap by 4 nucleotides PP_4015 and PP_4016 are separated by 137 nucleotides PP_4016 and PP_4017 are separated by 75 nucleotides
PP_4014: PP_4014 - tRNA-specific 2-thiouridylase MnmA, at 4,525,070 to 4,526,194
_4014
PP_4015: PP_4015 - HflD-like high frequency lysogenization protein, at 4,526,191 to 4,526,817
_4015
PP_4016: PP_4016 - 5'-phosphoribosyl-4-(N-succinocarboxamide)-5- aminoimidazole lyase (adenylosuccinate lyase), at 4,526,955 to 4,528,325
_4016
PP_4017: PP_4017 - conserved protein of unknown function, at 4,528,401 to 4,529,573
_4017
Position (kb)
4526
4527
4528
4529 Strain fitness (log2 ratio)
-1
0
1
2 at 4526.596 kb on + strand, within PP_4015 at 4526.596 kb on + strand, within PP_4015 at 4526.596 kb on + strand, within PP_4015 at 4526.596 kb on + strand at 4526.597 kb on - strand, within PP_4015 at 4526.597 kb on - strand, within PP_4015 at 4526.597 kb on - strand, within PP_4015 at 4526.597 kb on - strand, within PP_4015 at 4526.597 kb on - strand, within PP_4015 at 4526.910 kb on + strand at 4526.910 kb on + strand at 4526.910 kb on + strand at 4526.915 kb on + strand at 4528.323 kb on + strand at 4528.323 kb on + strand at 4528.339 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.1x with 55.5 mM D-Glucose; pH 4.6 remove 4,526,596 + PP_4015 0.65 -1.5 4,526,596 + PP_4015 0.65 +1.5 4,526,596 + PP_4015 0.65 +1.3 4,526,596 + -0.8 4,526,597 - PP_4015 0.65 +1.8 4,526,597 - PP_4015 0.65 -0.0 4,526,597 - PP_4015 0.65 -1.5 4,526,597 - PP_4015 0.65 -0.4 4,526,597 - PP_4015 0.65 -1.5 4,526,910 + +0.2 4,526,910 + +0.2 4,526,910 + -0.1 4,526,915 + +0.2 4,528,323 + -0.4 4,528,323 + +1.5 4,528,339 + +0.2
Or see this region's nucleotide sequence