Experiment: LB 0.1x with 55.5 mM D-Glucose; pH 4.6
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0155 and PP_5747 are separated by 2 nucleotides PP_5747 and PP_0156 are separated by 11 nucleotides PP_0156 and PP_0157 are separated by 419 nucleotides
PP_0155: PP_0155 - pyridine nucleotide transhydrogenase, beta subunit, at 164,020 to 165,456
_0155
PP_5747: PP_5747 - pyridine nucleotide transhydrogenase subunit alpha, at 165,459 to 165,773
_5747
PP_0156: PP_0156 - pyridine nucleotide transhydrogenase subunit alpha, at 165,785 to 166,906
_0156
PP_0157: PP_0157 - transcriptional activator of gcdH, LysR family, at 167,326 to 168,243
_0157
Position (kb) 165
166
167 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 164.799 kb on + strand, within PP_0155 at 164.799 kb on + strand, within PP_0155 at 164.855 kb on + strand, within PP_0155 at 164.855 kb on + strand, within PP_0155 at 164.855 kb on + strand, within PP_0155 at 164.856 kb on - strand, within PP_0155 at 164.856 kb on - strand, within PP_0155 at 164.856 kb on - strand, within PP_0155 at 164.856 kb on - strand, within PP_0155 at 164.856 kb on - strand, within PP_0155 at 164.942 kb on - strand, within PP_0155 at 164.972 kb on + strand, within PP_0155 at 164.972 kb on + strand, within PP_0155 at 165.018 kb on + strand, within PP_0155 at 165.018 kb on + strand, within PP_0155 at 165.018 kb on + strand, within PP_0155 at 165.019 kb on - strand, within PP_0155 at 165.019 kb on - strand, within PP_0155 at 165.019 kb on - strand, within PP_0155 at 165.260 kb on + strand, within PP_0155 at 165.260 kb on + strand, within PP_0155 at 165.297 kb on + strand, within PP_0155 at 165.297 kb on + strand, within PP_0155 at 165.297 kb on + strand, within PP_0155 at 165.298 kb on - strand, within PP_0155 at 165.298 kb on - strand, within PP_0155 at 165.298 kb on - strand, within PP_0155 at 165.298 kb on - strand, within PP_0155 at 165.299 kb on + strand, within PP_0155 at 165.299 kb on + strand, within PP_0155 at 165.300 kb on - strand, within PP_0155 at 165.300 kb on - strand, within PP_0155 at 165.428 kb on + strand at 165.428 kb on + strand at 165.429 kb on - strand at 165.460 kb on + strand at 165.461 kb on - strand at 165.461 kb on - strand at 165.703 kb on + strand, within PP_5747 at 165.704 kb on - strand, within PP_5747 at 165.712 kb on + strand, within PP_5747 at 165.712 kb on + strand, within PP_5747 at 165.712 kb on + strand, within PP_5747 at 165.712 kb on + strand, within PP_5747 at 165.712 kb on + strand, within PP_5747 at 165.712 kb on + strand, within PP_5747 at 165.712 kb on + strand, within PP_5747 at 165.713 kb on - strand, within PP_5747 at 165.713 kb on - strand, within PP_5747 at 165.713 kb on - strand, within PP_5747 at 165.713 kb on - strand, within PP_5747 at 165.713 kb on - strand, within PP_5747 at 165.743 kb on - strand at 165.787 kb on - strand at 165.918 kb on + strand, within PP_0156 at 165.918 kb on + strand, within PP_0156 at 165.919 kb on - strand, within PP_0156 at 166.400 kb on + strand, within PP_0156 at 166.464 kb on + strand, within PP_0156 at 166.464 kb on + strand, within PP_0156 at 166.465 kb on - strand, within PP_0156 at 166.465 kb on - strand, within PP_0156 at 166.465 kb on - strand, within PP_0156 at 166.494 kb on + strand, within PP_0156 at 166.494 kb on + strand, within PP_0156 at 166.495 kb on - strand, within PP_0156 at 166.495 kb on - strand, within PP_0156 at 166.580 kb on - strand, within PP_0156 at 166.700 kb on + strand, within PP_0156 at 166.716 kb on + strand, within PP_0156 at 166.716 kb on + strand, within PP_0156 at 166.910 kb on + strand at 166.910 kb on + strand at 166.910 kb on + strand at 166.910 kb on + strand at 166.910 kb on + strand at 166.911 kb on - strand at 166.911 kb on - strand at 166.911 kb on - strand at 166.940 kb on + strand at 166.941 kb on - strand at 166.941 kb on - strand at 167.076 kb on + strand at 167.076 kb on + strand at 167.185 kb on - strand at 167.191 kb on - strand at 167.208 kb on + strand at 167.225 kb on + strand at 167.239 kb on - strand at 167.285 kb on - strand at 167.285 kb on - strand at 167.285 kb on - strand at 167.286 kb on + strand at 167.286 kb on + strand at 167.286 kb on + strand at 167.286 kb on + strand at 167.286 kb on + strand at 167.286 kb on + strand at 167.286 kb on + strand at 167.286 kb on + strand at 167.286 kb on + strand at 167.287 kb on - strand at 167.287 kb on - strand at 167.287 kb on - strand at 167.290 kb on + strand at 167.290 kb on + strand at 167.290 kb on + strand at 167.290 kb on + strand at 167.290 kb on + strand at 167.290 kb on + strand at 167.290 kb on + strand at 167.290 kb on + strand at 167.290 kb on + strand at 167.290 kb on + strand at 167.290 kb on + strand at 167.290 kb on + strand at 167.290 kb on + strand at 167.290 kb on + strand at 167.290 kb on + strand at 167.290 kb on + strand at 167.291 kb on - strand at 167.291 kb on - strand at 167.291 kb on - strand at 167.291 kb on - strand at 167.291 kb on - strand at 167.291 kb on - strand at 167.291 kb on - strand at 167.291 kb on - strand at 167.291 kb on - strand at 167.291 kb on - strand at 167.298 kb on + strand at 167.307 kb on - strand at 167.352 kb on + strand at 167.352 kb on + strand at 167.352 kb on + strand at 167.352 kb on + strand at 167.352 kb on + strand at 167.353 kb on - strand at 167.353 kb on - strand at 167.353 kb on - strand at 167.353 kb on - strand at 167.356 kb on + strand at 167.356 kb on + strand at 167.356 kb on + strand at 167.356 kb on + strand at 167.356 kb on + strand at 167.356 kb on + strand at 167.357 kb on - strand at 167.357 kb on - strand at 167.357 kb on - strand at 167.357 kb on - strand at 167.357 kb on - strand at 167.357 kb on - strand at 167.360 kb on + strand at 167.360 kb on + strand at 167.361 kb on - strand at 167.361 kb on - strand at 167.378 kb on - strand at 167.441 kb on + strand, within PP_0157 at 167.441 kb on + strand, within PP_0157 at 167.442 kb on - strand, within PP_0157 at 167.442 kb on - strand, within PP_0157 at 167.442 kb on - strand, within PP_0157 at 167.445 kb on - strand, within PP_0157 at 167.445 kb on - strand, within PP_0157 at 167.445 kb on - strand, within PP_0157 at 167.458 kb on - strand, within PP_0157 at 167.476 kb on - strand, within PP_0157 at 167.527 kb on + strand, within PP_0157 at 167.528 kb on - strand, within PP_0157 at 167.578 kb on + strand, within PP_0157 at 167.578 kb on + strand, within PP_0157 at 167.578 kb on + strand, within PP_0157 at 167.579 kb on - strand, within PP_0157 at 167.579 kb on - strand, within PP_0157 at 167.585 kb on + strand, within PP_0157 at 167.586 kb on - strand, within PP_0157 at 167.807 kb on + strand, within PP_0157 at 167.808 kb on - strand, within PP_0157 at 167.808 kb on - strand, within PP_0157 at 167.827 kb on - strand, within PP_0157 at 167.827 kb on - strand, within PP_0157
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.1x with 55.5 mM D-Glucose; pH 4.6 remove 164,799 + PP_0155 0.54 +1.2 164,799 + PP_0155 0.54 -0.3 164,855 + PP_0155 0.58 +0.6 164,855 + PP_0155 0.58 +0.2 164,855 + PP_0155 0.58 +0.2 164,856 - PP_0155 0.58 -0.2 164,856 - PP_0155 0.58 -0.7 164,856 - PP_0155 0.58 +0.7 164,856 - PP_0155 0.58 +0.5 164,856 - PP_0155 0.58 +0.6 164,942 - PP_0155 0.64 +0.4 164,972 + PP_0155 0.66 +0.6 164,972 + PP_0155 0.66 -1.5 165,018 + PP_0155 0.69 +0.9 165,018 + PP_0155 0.69 +0.8 165,018 + PP_0155 0.69 +0.9 165,019 - PP_0155 0.70 +0.6 165,019 - PP_0155 0.70 +0.5 165,019 - PP_0155 0.70 -0.6 165,260 + PP_0155 0.86 +0.7 165,260 + PP_0155 0.86 -0.9 165,297 + PP_0155 0.89 +0.7 165,297 + PP_0155 0.89 -0.3 165,297 + PP_0155 0.89 -0.2 165,298 - PP_0155 0.89 +0.2 165,298 - PP_0155 0.89 +0.7 165,298 - PP_0155 0.89 -0.1 165,298 - PP_0155 0.89 +1.8 165,299 + PP_0155 0.89 +0.1 165,299 + PP_0155 0.89 -1.1 165,300 - PP_0155 0.89 +0.3 165,300 - PP_0155 0.89 +0.4 165,428 + -0.0 165,428 + -0.6 165,429 - +0.0 165,460 + -0.7 165,461 - +0.5 165,461 - -0.9 165,703 + PP_5747 0.77 -0.6 165,704 - PP_5747 0.78 -0.7 165,712 + PP_5747 0.80 +0.5 165,712 + PP_5747 0.80 +1.1 165,712 + PP_5747 0.80 +0.3 165,712 + PP_5747 0.80 -0.6 165,712 + PP_5747 0.80 +1.3 165,712 + PP_5747 0.80 -0.5 165,712 + PP_5747 0.80 -0.5 165,713 - PP_5747 0.81 -2.3 165,713 - PP_5747 0.81 +0.0 165,713 - PP_5747 0.81 -1.3 165,713 - PP_5747 0.81 +0.4 165,713 - PP_5747 0.81 +1.0 165,743 - -0.0 165,787 - -1.7 165,918 + PP_0156 0.12 +1.0 165,918 + PP_0156 0.12 -0.1 165,919 - PP_0156 0.12 +0.5 166,400 + PP_0156 0.55 +0.5 166,464 + PP_0156 0.61 +0.3 166,464 + PP_0156 0.61 -0.1 166,465 - PP_0156 0.61 -0.3 166,465 - PP_0156 0.61 +0.2 166,465 - PP_0156 0.61 +0.9 166,494 + PP_0156 0.63 +0.0 166,494 + PP_0156 0.63 +0.6 166,495 - PP_0156 0.63 +0.3 166,495 - PP_0156 0.63 +1.6 166,580 - PP_0156 0.71 +0.5 166,700 + PP_0156 0.82 +0.5 166,716 + PP_0156 0.83 -0.1 166,716 + PP_0156 0.83 +1.3 166,910 + +1.0 166,910 + +0.3 166,910 + +0.3 166,910 + +0.2 166,910 + +0.2 166,911 - +0.0 166,911 - +0.8 166,911 - +0.0 166,940 + +0.1 166,941 - -0.3 166,941 - +2.3 167,076 + +0.6 167,076 + -0.1 167,185 - -1.0 167,191 - +0.2 167,208 + +1.0 167,225 + +0.8 167,239 - -1.3 167,285 - +0.1 167,285 - -0.2 167,285 - +1.0 167,286 + +1.8 167,286 + -0.1 167,286 + +0.3 167,286 + +0.3 167,286 + -0.6 167,286 + -2.2 167,286 + -1.0 167,286 + +0.3 167,286 + -1.3 167,287 - -0.7 167,287 - +1.3 167,287 - +0.7 167,290 + +1.9 167,290 + -0.7 167,290 + -1.0 167,290 + -0.3 167,290 + -0.2 167,290 + +0.6 167,290 + -0.7 167,290 + -0.1 167,290 + -0.0 167,290 + +0.3 167,290 + -0.1 167,290 + +0.3 167,290 + -0.0 167,290 + +1.3 167,290 + +0.5 167,290 + +1.3 167,291 - +0.8 167,291 - -2.5 167,291 - -0.2 167,291 - +0.2 167,291 - +0.2 167,291 - -0.2 167,291 - +0.5 167,291 - -1.7 167,291 - -2.3 167,291 - -1.3 167,298 + +1.0 167,307 - +1.0 167,352 + -3.0 167,352 + +0.5 167,352 + -0.0 167,352 + -1.3 167,352 + -0.4 167,353 - +0.2 167,353 - +0.8 167,353 - -0.1 167,353 - +0.2 167,356 + -0.2 167,356 + -0.5 167,356 + -0.3 167,356 + +0.1 167,356 + +0.2 167,356 + +1.2 167,357 - +1.4 167,357 - +1.9 167,357 - +0.4 167,357 - +0.5 167,357 - -2.5 167,357 - +0.3 167,360 + +1.3 167,360 + +0.6 167,361 - -0.7 167,361 - +1.3 167,378 - -0.2 167,441 + PP_0157 0.13 +0.3 167,441 + PP_0157 0.13 -0.5 167,442 - PP_0157 0.13 +0.3 167,442 - PP_0157 0.13 +1.6 167,442 - PP_0157 0.13 +0.3 167,445 - PP_0157 0.13 -0.7 167,445 - PP_0157 0.13 +0.6 167,445 - PP_0157 0.13 +0.2 167,458 - PP_0157 0.14 -0.3 167,476 - PP_0157 0.16 +0.5 167,527 + PP_0157 0.22 +0.2 167,528 - PP_0157 0.22 +1.7 167,578 + PP_0157 0.27 +0.9 167,578 + PP_0157 0.27 -0.8 167,578 + PP_0157 0.27 +0.1 167,579 - PP_0157 0.28 -0.2 167,579 - PP_0157 0.28 -0.1 167,585 + PP_0157 0.28 +0.3 167,586 - PP_0157 0.28 -0.1 167,807 + PP_0157 0.52 +0.0 167,808 - PP_0157 0.53 -0.9 167,808 - PP_0157 0.53 -0.3 167,827 - PP_0157 0.55 +0.8 167,827 - PP_0157 0.55 +0.6
Or see this region's nucleotide sequence