Experiment: LB Anaerobic with Doxycycline hyclate 0.00000078125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pqiA and pqiB are separated by 4 nucleotides pqiB and ymbA overlap by 4 nucleotides ymbA and rmf are separated by 255 nucleotides
b0950: pqiA - paraquat-inducible membrane protein A (NCBI), at 1,011,224 to 1,012,477
pqiA
b0951: pqiB - paraquat-inducible protein B (NCBI), at 1,012,482 to 1,014,122
pqiB
b0952: ymbA - conserved protein (RefSeq), at 1,014,119 to 1,014,682
ymbA
b0953: rmf - ribosome modulation factor (NCBI), at 1,014,938 to 1,015,105
rmf
Position (kb)
1012
1013
1014
1015 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1011.607 kb on - strand, within pqiA at 1011.620 kb on - strand, within pqiA at 1011.748 kb on + strand, within pqiA at 1011.794 kb on + strand, within pqiA at 1011.876 kb on + strand, within pqiA at 1011.956 kb on - strand, within pqiA at 1011.982 kb on + strand, within pqiA at 1012.008 kb on - strand, within pqiA at 1012.008 kb on - strand, within pqiA at 1012.011 kb on + strand, within pqiA at 1012.067 kb on + strand, within pqiA at 1012.067 kb on + strand, within pqiA at 1012.131 kb on - strand, within pqiA at 1012.166 kb on - strand, within pqiA at 1012.166 kb on - strand, within pqiA at 1012.181 kb on - strand, within pqiA at 1012.260 kb on - strand, within pqiA at 1012.323 kb on - strand, within pqiA at 1012.323 kb on - strand, within pqiA at 1012.450 kb on + strand at 1012.450 kb on + strand at 1012.460 kb on + strand at 1012.570 kb on - strand at 1012.570 kb on - strand at 1012.625 kb on - strand at 1012.625 kb on - strand at 1012.672 kb on + strand, within pqiB at 1012.805 kb on + strand, within pqiB at 1012.847 kb on + strand, within pqiB at 1012.865 kb on - strand, within pqiB at 1012.865 kb on - strand, within pqiB at 1013.007 kb on + strand, within pqiB at 1013.020 kb on - strand, within pqiB at 1013.020 kb on - strand, within pqiB at 1013.031 kb on - strand, within pqiB at 1013.108 kb on - strand, within pqiB at 1013.119 kb on + strand, within pqiB at 1013.144 kb on + strand, within pqiB at 1013.168 kb on - strand, within pqiB at 1013.178 kb on - strand, within pqiB at 1013.178 kb on - strand, within pqiB at 1013.211 kb on - strand, within pqiB at 1013.329 kb on - strand, within pqiB at 1013.374 kb on + strand, within pqiB at 1013.374 kb on + strand, within pqiB at 1013.389 kb on - strand, within pqiB at 1013.399 kb on + strand, within pqiB at 1013.632 kb on - strand, within pqiB at 1013.632 kb on - strand, within pqiB at 1013.633 kb on - strand, within pqiB at 1013.634 kb on - strand, within pqiB at 1013.635 kb on - strand, within pqiB at 1013.730 kb on + strand, within pqiB at 1013.738 kb on - strand, within pqiB at 1013.811 kb on - strand, within pqiB at 1013.811 kb on - strand, within pqiB at 1013.858 kb on - strand, within pqiB at 1013.858 kb on - strand, within pqiB at 1013.949 kb on - strand, within pqiB at 1013.955 kb on + strand, within pqiB at 1013.955 kb on + strand, within pqiB at 1014.064 kb on - strand at 1014.064 kb on - strand at 1014.159 kb on - strand at 1014.206 kb on + strand, within ymbA at 1014.216 kb on + strand, within ymbA at 1014.216 kb on + strand, within ymbA at 1014.263 kb on + strand, within ymbA at 1014.268 kb on + strand, within ymbA at 1014.303 kb on - strand, within ymbA at 1014.428 kb on - strand, within ymbA at 1014.428 kb on - strand, within ymbA at 1014.587 kb on + strand, within ymbA at 1014.588 kb on + strand, within ymbA at 1014.588 kb on + strand, within ymbA at 1014.619 kb on - strand, within ymbA at 1014.959 kb on + strand, within rmf at 1014.974 kb on + strand, within rmf at 1014.974 kb on + strand, within rmf at 1015.089 kb on + strand at 1015.097 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Doxycycline hyclate 0.00000078125 mM remove 1,011,607 - pqiA b0950 0.31 -0.7 1,011,620 - pqiA b0950 0.32 -0.9 1,011,748 + pqiA b0950 0.42 -1.9 1,011,794 + pqiA b0950 0.45 -0.9 1,011,876 + pqiA b0950 0.52 -1.9 1,011,956 - pqiA b0950 0.58 +0.5 1,011,982 + pqiA b0950 0.60 -0.3 1,012,008 - pqiA b0950 0.63 -1.0 1,012,008 - pqiA b0950 0.63 +0.7 1,012,011 + pqiA b0950 0.63 -1.4 1,012,067 + pqiA b0950 0.67 -1.3 1,012,067 + pqiA b0950 0.67 -1.3 1,012,131 - pqiA b0950 0.72 -0.9 1,012,166 - pqiA b0950 0.75 -1.2 1,012,166 - pqiA b0950 0.75 -0.3 1,012,181 - pqiA b0950 0.76 +0.1 1,012,260 - pqiA b0950 0.83 -1.6 1,012,323 - pqiA b0950 0.88 -2.1 1,012,323 - pqiA b0950 0.88 +0.1 1,012,450 + +0.1 1,012,450 + +0.2 1,012,460 + +0.7 1,012,570 - +1.3 1,012,570 - -0.1 1,012,625 - -1.3 1,012,625 - +0.7 1,012,672 + pqiB b0951 0.12 -1.5 1,012,805 + pqiB b0951 0.20 -1.6 1,012,847 + pqiB b0951 0.22 +0.4 1,012,865 - pqiB b0951 0.23 -2.1 1,012,865 - pqiB b0951 0.23 -0.3 1,013,007 + pqiB b0951 0.32 -0.8 1,013,020 - pqiB b0951 0.33 -0.6 1,013,020 - pqiB b0951 0.33 -3.4 1,013,031 - pqiB b0951 0.33 -1.2 1,013,108 - pqiB b0951 0.38 -0.6 1,013,119 + pqiB b0951 0.39 -1.4 1,013,144 + pqiB b0951 0.40 +1.9 1,013,168 - pqiB b0951 0.42 +0.0 1,013,178 - pqiB b0951 0.42 -0.1 1,013,178 - pqiB b0951 0.42 +0.2 1,013,211 - pqiB b0951 0.44 -1.8 1,013,329 - pqiB b0951 0.52 -0.9 1,013,374 + pqiB b0951 0.54 +0.1 1,013,374 + pqiB b0951 0.54 -1.3 1,013,389 - pqiB b0951 0.55 -0.3 1,013,399 + pqiB b0951 0.56 -0.5 1,013,632 - pqiB b0951 0.70 +0.5 1,013,632 - pqiB b0951 0.70 -1.1 1,013,633 - pqiB b0951 0.70 -0.9 1,013,634 - pqiB b0951 0.70 -0.9 1,013,635 - pqiB b0951 0.70 -1.4 1,013,730 + pqiB b0951 0.76 -0.6 1,013,738 - pqiB b0951 0.77 -1.1 1,013,811 - pqiB b0951 0.81 -1.1 1,013,811 - pqiB b0951 0.81 -1.2 1,013,858 - pqiB b0951 0.84 -1.3 1,013,858 - pqiB b0951 0.84 -0.9 1,013,949 - pqiB b0951 0.89 -1.6 1,013,955 + pqiB b0951 0.90 -0.1 1,013,955 + pqiB b0951 0.90 +0.1 1,014,064 - -0.9 1,014,064 - -1.9 1,014,159 - +0.7 1,014,206 + ymbA b0952 0.15 -1.5 1,014,216 + ymbA b0952 0.17 -0.5 1,014,216 + ymbA b0952 0.17 +1.1 1,014,263 + ymbA b0952 0.26 -0.8 1,014,268 + ymbA b0952 0.26 -0.5 1,014,303 - ymbA b0952 0.33 -0.3 1,014,428 - ymbA b0952 0.55 +1.5 1,014,428 - ymbA b0952 0.55 -0.6 1,014,587 + ymbA b0952 0.83 -0.9 1,014,588 + ymbA b0952 0.83 -0.6 1,014,588 + ymbA b0952 0.83 -0.7 1,014,619 - ymbA b0952 0.89 -0.0 1,014,959 + rmf b0953 0.12 +0.3 1,014,974 + rmf b0953 0.21 -0.3 1,014,974 + rmf b0953 0.21 +0.2 1,015,089 + +0.0 1,015,097 - -0.8
Or see this region's nucleotide sequence