Experiment: LB Anaerobic with Doxycycline hyclate 0.00000078125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt nagB and nagE are separated by 332 nucleotides nagE and glnS are separated by 202 nucleotides
b0678: nagB - glucosamine-6-phosphate deaminase (NCBI), at 702,034 to 702,834
nagB
b0679: nagE - fused N-acetyl glucosamine specific PTS enzyme: IIC, IIB , and IIA components (NCBI), at 703,167 to 705,113
nagE
b0680: glnS - glutaminyl-tRNA synthetase (NCBI), at 705,316 to 706,980
glnS
Position (kb) 703
704
705
706 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 702.180 kb on - strand, within nagB at 702.217 kb on - strand, within nagB at 702.250 kb on + strand, within nagB at 702.346 kb on + strand, within nagB at 702.619 kb on - strand, within nagB at 702.624 kb on + strand, within nagB at 702.624 kb on + strand, within nagB at 702.769 kb on + strand at 702.794 kb on + strand at 702.804 kb on + strand at 702.823 kb on + strand at 702.823 kb on + strand at 702.881 kb on + strand at 702.966 kb on + strand at 703.054 kb on + strand at 703.054 kb on + strand at 703.064 kb on + strand at 703.087 kb on + strand at 703.193 kb on + strand at 703.193 kb on + strand at 703.211 kb on + strand at 703.420 kb on + strand, within nagE at 703.464 kb on - strand, within nagE at 703.634 kb on + strand, within nagE at 703.639 kb on - strand, within nagE at 703.663 kb on + strand, within nagE at 703.692 kb on - strand, within nagE at 703.711 kb on - strand, within nagE at 703.711 kb on - strand, within nagE at 703.742 kb on + strand, within nagE at 703.786 kb on - strand, within nagE at 703.786 kb on - strand, within nagE at 703.797 kb on + strand, within nagE at 703.815 kb on + strand, within nagE at 703.817 kb on - strand, within nagE at 703.865 kb on + strand, within nagE at 703.926 kb on + strand, within nagE at 703.926 kb on + strand, within nagE at 704.039 kb on - strand, within nagE at 704.042 kb on - strand, within nagE at 704.042 kb on - strand, within nagE at 704.043 kb on + strand, within nagE at 704.119 kb on - strand, within nagE at 704.119 kb on - strand, within nagE at 704.153 kb on - strand, within nagE at 704.356 kb on + strand, within nagE at 704.464 kb on - strand, within nagE at 704.500 kb on - strand, within nagE at 704.513 kb on - strand, within nagE at 704.513 kb on - strand, within nagE at 704.532 kb on + strand, within nagE at 704.648 kb on - strand, within nagE at 704.648 kb on - strand, within nagE at 704.648 kb on - strand, within nagE at 704.686 kb on - strand, within nagE at 704.726 kb on + strand, within nagE at 704.787 kb on - strand, within nagE at 704.800 kb on - strand, within nagE at 704.876 kb on + strand, within nagE at 704.876 kb on + strand, within nagE at 704.922 kb on + strand at 704.942 kb on - strand at 705.090 kb on - strand at 705.090 kb on - strand at 705.257 kb on - strand at 705.281 kb on + strand at 705.302 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Doxycycline hyclate 0.00000078125 mM remove 702,180 - nagB b0678 0.18 +0.7 702,217 - nagB b0678 0.23 -0.8 702,250 + nagB b0678 0.27 +0.9 702,346 + nagB b0678 0.39 -0.1 702,619 - nagB b0678 0.73 +0.2 702,624 + nagB b0678 0.74 -0.3 702,624 + nagB b0678 0.74 +0.7 702,769 + +0.9 702,794 + +0.0 702,804 + +1.2 702,823 + -0.1 702,823 + +0.9 702,881 + -0.1 702,966 + -0.1 703,054 + +0.9 703,054 + +0.2 703,064 + -0.2 703,087 + +1.1 703,193 + -1.9 703,193 + -0.3 703,211 + -0.5 703,420 + nagE b0679 0.13 +0.2 703,464 - nagE b0679 0.15 +0.8 703,634 + nagE b0679 0.24 -0.7 703,639 - nagE b0679 0.24 -0.9 703,663 + nagE b0679 0.25 -0.3 703,692 - nagE b0679 0.27 -1.0 703,711 - nagE b0679 0.28 +0.5 703,711 - nagE b0679 0.28 +0.2 703,742 + nagE b0679 0.30 +0.5 703,786 - nagE b0679 0.32 +0.7 703,786 - nagE b0679 0.32 -0.7 703,797 + nagE b0679 0.32 -1.1 703,815 + nagE b0679 0.33 -0.7 703,817 - nagE b0679 0.33 -0.1 703,865 + nagE b0679 0.36 -0.8 703,926 + nagE b0679 0.39 -3.2 703,926 + nagE b0679 0.39 -0.4 704,039 - nagE b0679 0.45 -0.1 704,042 - nagE b0679 0.45 -1.2 704,042 - nagE b0679 0.45 +1.3 704,043 + nagE b0679 0.45 -1.0 704,119 - nagE b0679 0.49 -0.5 704,119 - nagE b0679 0.49 -0.6 704,153 - nagE b0679 0.51 -0.2 704,356 + nagE b0679 0.61 -1.1 704,464 - nagE b0679 0.67 -0.9 704,500 - nagE b0679 0.68 -0.9 704,513 - nagE b0679 0.69 +0.2 704,513 - nagE b0679 0.69 +0.9 704,532 + nagE b0679 0.70 -0.6 704,648 - nagE b0679 0.76 -0.9 704,648 - nagE b0679 0.76 -1.5 704,648 - nagE b0679 0.76 +1.8 704,686 - nagE b0679 0.78 -0.8 704,726 + nagE b0679 0.80 -1.1 704,787 - nagE b0679 0.83 -2.1 704,800 - nagE b0679 0.84 -0.1 704,876 + nagE b0679 0.88 -0.7 704,876 + nagE b0679 0.88 -2.0 704,922 + -0.9 704,942 - +0.1 705,090 - +0.1 705,090 - -0.7 705,257 - +0.9 705,281 + -0.4 705,302 + +1.9
Or see this region's nucleotide sequence