Experiment: LB Anaerobic with Doxycycline hyclate 0.00000078125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt allA and allR are separated by 77 nucleotides allR and gcl are separated by 89 nucleotides gcl and hyi are separated by 12 nucleotides hyi and glxR are separated by 99 nucleotides
b0505: allA - ureidoglycolate hydrolase (NCBI), at 531,675 to 532,157
allA
b0506: allR - DNA-binding transcriptional repressor (NCBI), at 532,235 to 533,050
allR
b0507: gcl - glyoxylate carboligase (NCBI), at 533,140 to 534,921
gcl
b0508: hyi - hydroxypyruvate isomerase (NCBI), at 534,934 to 535,710
hyi
b0509: glxR - tartronate semialdehyde reductase, NADH-dependent (NCBI), at 535,810 to 536,688
glxR
Position (kb) 533
534
535 Strain fitness (log2 ratio) -2
-1
0
1
2 at 532.199 kb on + strand at 532.216 kb on - strand at 532.221 kb on + strand at 532.278 kb on + strand at 532.278 kb on + strand at 532.497 kb on - strand, within allR at 532.513 kb on - strand, within allR at 532.560 kb on + strand, within allR at 532.616 kb on + strand, within allR at 532.642 kb on - strand, within allR at 532.657 kb on - strand, within allR at 532.677 kb on + strand, within allR at 532.677 kb on + strand, within allR at 532.798 kb on - strand, within allR at 532.798 kb on - strand, within allR at 532.811 kb on + strand, within allR at 532.811 kb on + strand, within allR at 532.840 kb on + strand, within allR at 532.840 kb on + strand, within allR at 532.992 kb on - strand at 533.167 kb on + strand at 533.167 kb on + strand at 533.171 kb on + strand at 533.171 kb on + strand at 533.245 kb on + strand at 533.246 kb on - strand at 533.423 kb on + strand, within gcl at 533.444 kb on + strand, within gcl at 533.561 kb on - strand, within gcl at 533.606 kb on + strand, within gcl at 533.754 kb on + strand, within gcl at 533.754 kb on + strand, within gcl at 533.805 kb on + strand, within gcl at 533.859 kb on - strand, within gcl at 533.869 kb on + strand, within gcl at 533.881 kb on + strand, within gcl at 533.922 kb on + strand, within gcl at 533.996 kb on - strand, within gcl at 534.135 kb on + strand, within gcl at 534.199 kb on - strand, within gcl at 534.217 kb on - strand, within gcl at 534.263 kb on + strand, within gcl at 534.279 kb on - strand, within gcl at 534.279 kb on - strand, within gcl at 534.281 kb on - strand, within gcl at 534.314 kb on + strand, within gcl at 534.539 kb on + strand, within gcl at 534.539 kb on + strand, within gcl at 534.544 kb on - strand, within gcl at 534.557 kb on + strand, within gcl at 534.557 kb on + strand, within gcl at 534.571 kb on - strand, within gcl at 534.661 kb on + strand, within gcl at 534.670 kb on - strand, within gcl at 534.672 kb on + strand, within gcl at 534.696 kb on + strand, within gcl at 534.740 kb on + strand, within gcl at 534.740 kb on + strand, within gcl at 534.740 kb on + strand, within gcl at 534.756 kb on + strand at 534.756 kb on + strand at 534.830 kb on + strand at 534.830 kb on + strand at 534.830 kb on + strand at 534.833 kb on + strand at 534.833 kb on + strand at 534.835 kb on + strand at 534.835 kb on + strand at 534.843 kb on - strand at 534.865 kb on + strand at 534.868 kb on + strand at 534.868 kb on + strand at 534.868 kb on + strand at 534.878 kb on + strand at 534.878 kb on + strand at 534.889 kb on + strand at 534.891 kb on + strand at 534.891 kb on + strand at 534.892 kb on + strand at 534.892 kb on + strand at 534.899 kb on - strand at 534.911 kb on + strand at 534.912 kb on + strand at 534.912 kb on + strand at 535.001 kb on + strand at 535.011 kb on + strand at 535.128 kb on - strand, within hyi at 535.143 kb on - strand, within hyi at 535.159 kb on + strand, within hyi at 535.162 kb on - strand, within hyi at 535.162 kb on - strand, within hyi at 535.207 kb on + strand, within hyi at 535.207 kb on + strand, within hyi at 535.207 kb on + strand, within hyi at 535.214 kb on + strand, within hyi at 535.214 kb on + strand, within hyi at 535.267 kb on + strand, within hyi at 535.356 kb on + strand, within hyi at 535.403 kb on + strand, within hyi at 535.435 kb on + strand, within hyi at 535.485 kb on + strand, within hyi at 535.511 kb on + strand, within hyi at 535.532 kb on - strand, within hyi at 535.681 kb on + strand at 535.686 kb on + strand at 535.691 kb on + strand at 535.691 kb on + strand at 535.696 kb on + strand at 535.704 kb on - strand at 535.717 kb on + strand at 535.717 kb on + strand at 535.722 kb on + strand at 535.759 kb on - strand at 535.759 kb on - strand at 535.760 kb on + strand at 535.760 kb on + strand at 535.760 kb on + strand at 535.841 kb on + strand at 535.849 kb on - strand at 535.860 kb on - strand at 535.882 kb on + strand at 535.890 kb on - strand at 535.892 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Doxycycline hyclate 0.00000078125 mM remove 532,199 + -0.9 532,216 - -0.4 532,221 + +0.1 532,278 + -1.3 532,278 + -1.8 532,497 - allR b0506 0.32 +0.4 532,513 - allR b0506 0.34 +1.0 532,560 + allR b0506 0.40 -0.6 532,616 + allR b0506 0.47 -0.7 532,642 - allR b0506 0.50 +0.2 532,657 - allR b0506 0.52 -1.1 532,677 + allR b0506 0.54 +0.0 532,677 + allR b0506 0.54 -1.2 532,798 - allR b0506 0.69 -1.8 532,798 - allR b0506 0.69 +1.0 532,811 + allR b0506 0.71 -1.2 532,811 + allR b0506 0.71 +0.5 532,840 + allR b0506 0.74 -0.2 532,840 + allR b0506 0.74 -0.6 532,992 - +0.8 533,167 + -1.0 533,167 + +0.5 533,171 + -0.1 533,171 + -1.1 533,245 + -0.2 533,246 - -0.3 533,423 + gcl b0507 0.16 +0.6 533,444 + gcl b0507 0.17 +0.5 533,561 - gcl b0507 0.24 +0.1 533,606 + gcl b0507 0.26 +0.4 533,754 + gcl b0507 0.34 -0.7 533,754 + gcl b0507 0.34 +0.8 533,805 + gcl b0507 0.37 -0.1 533,859 - gcl b0507 0.40 -0.5 533,869 + gcl b0507 0.41 +1.2 533,881 + gcl b0507 0.42 -0.1 533,922 + gcl b0507 0.44 -0.0 533,996 - gcl b0507 0.48 +0.1 534,135 + gcl b0507 0.56 +0.8 534,199 - gcl b0507 0.59 +0.6 534,217 - gcl b0507 0.60 -0.0 534,263 + gcl b0507 0.63 +0.1 534,279 - gcl b0507 0.64 -0.3 534,279 - gcl b0507 0.64 -1.0 534,281 - gcl b0507 0.64 +0.3 534,314 + gcl b0507 0.66 +0.1 534,539 + gcl b0507 0.79 +0.0 534,539 + gcl b0507 0.79 +0.6 534,544 - gcl b0507 0.79 -1.8 534,557 + gcl b0507 0.80 +1.3 534,557 + gcl b0507 0.80 +0.6 534,571 - gcl b0507 0.80 -0.3 534,661 + gcl b0507 0.85 -0.4 534,670 - gcl b0507 0.86 +1.0 534,672 + gcl b0507 0.86 +0.1 534,696 + gcl b0507 0.87 -1.1 534,740 + gcl b0507 0.90 +0.3 534,740 + gcl b0507 0.90 +1.0 534,740 + gcl b0507 0.90 -0.3 534,756 + -0.0 534,756 + -0.1 534,830 + -0.3 534,830 + +0.7 534,830 + +2.6 534,833 + -0.7 534,833 + -2.0 534,835 + +0.1 534,835 + +1.4 534,843 - +1.0 534,865 + -0.4 534,868 + +0.5 534,868 + +0.3 534,868 + -1.6 534,878 + -0.3 534,878 + -0.6 534,889 + -0.4 534,891 + +0.0 534,891 + +0.4 534,892 + +0.3 534,892 + -1.6 534,899 - +1.0 534,911 + -1.0 534,912 + -0.8 534,912 + -1.3 535,001 + +1.0 535,011 + -0.6 535,128 - hyi b0508 0.25 -1.0 535,143 - hyi b0508 0.27 +1.0 535,159 + hyi b0508 0.29 +1.0 535,162 - hyi b0508 0.29 +1.0 535,162 - hyi b0508 0.29 +1.0 535,207 + hyi b0508 0.35 +0.6 535,207 + hyi b0508 0.35 +0.0 535,207 + hyi b0508 0.35 +2.0 535,214 + hyi b0508 0.36 +1.6 535,214 + hyi b0508 0.36 +0.2 535,267 + hyi b0508 0.43 +0.8 535,356 + hyi b0508 0.54 +0.0 535,403 + hyi b0508 0.60 +2.0 535,435 + hyi b0508 0.64 -0.6 535,485 + hyi b0508 0.71 +1.0 535,511 + hyi b0508 0.74 +1.0 535,532 - hyi b0508 0.77 +1.0 535,681 + -0.6 535,686 + -0.4 535,691 + +1.6 535,691 + -0.6 535,696 + -1.3 535,704 - +1.0 535,717 + +0.6 535,717 + +0.3 535,722 + -0.0 535,759 - +1.0 535,759 - -0.6 535,760 + +0.4 535,760 + -0.6 535,760 + +0.5 535,841 + +0.0 535,849 - -1.0 535,860 - -0.6 535,882 + -0.6 535,890 - -0.6 535,892 - -1.0
Or see this region's nucleotide sequence