Experiment: LB Anaerobic with Doxycycline hyclate 0.00000078125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yahG and yahI are separated by 421 nucleotides yahI and yahJ are separated by 9 nucleotides
b0321: yahG - hypothetical protein (NCBI), at 337,549 to 338,967
yahG
b0323: yahI - predicted carbamate kinase-like protein (NCBI), at 339,389 to 340,339
yahI
b0324: yahJ - deaminase (NCBI), at 340,349 to 341,731
yahJ
Position (kb) 339
340
341 Strain fitness (log2 ratio) -1
0
1
2 at 338.424 kb on - strand, within yahG at 338.429 kb on - strand, within yahG at 338.551 kb on - strand, within yahG at 338.656 kb on - strand, within yahG at 338.902 kb on + strand at 338.902 kb on + strand at 339.006 kb on - strand at 339.184 kb on - strand at 339.195 kb on + strand at 339.195 kb on + strand at 339.203 kb on - strand at 339.203 kb on - strand at 339.378 kb on - strand at 339.390 kb on - strand at 339.390 kb on - strand at 339.411 kb on - strand at 339.431 kb on + strand at 339.444 kb on - strand at 339.565 kb on + strand, within yahI at 339.565 kb on + strand, within yahI at 339.630 kb on + strand, within yahI at 339.630 kb on + strand, within yahI at 339.741 kb on - strand, within yahI at 339.798 kb on + strand, within yahI at 339.806 kb on - strand, within yahI at 340.025 kb on + strand, within yahI at 340.025 kb on + strand, within yahI at 340.047 kb on - strand, within yahI at 340.170 kb on - strand, within yahI at 340.174 kb on - strand, within yahI at 340.174 kb on - strand, within yahI at 340.257 kb on + strand at 340.257 kb on + strand at 340.320 kb on + strand at 340.320 kb on + strand at 340.394 kb on + strand at 340.448 kb on + strand at 340.461 kb on + strand at 340.522 kb on - strand, within yahJ at 340.573 kb on - strand, within yahJ at 340.573 kb on - strand, within yahJ at 340.675 kb on + strand, within yahJ at 340.675 kb on + strand, within yahJ at 340.776 kb on + strand, within yahJ at 340.952 kb on + strand, within yahJ at 341.087 kb on - strand, within yahJ at 341.087 kb on - strand, within yahJ at 341.124 kb on + strand, within yahJ at 341.139 kb on - strand, within yahJ at 341.141 kb on + strand, within yahJ
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Doxycycline hyclate 0.00000078125 mM remove 338,424 - yahG b0321 0.62 -0.2 338,429 - yahG b0321 0.62 +0.1 338,551 - yahG b0321 0.71 +0.6 338,656 - yahG b0321 0.78 -0.2 338,902 + +0.3 338,902 + +1.2 339,006 - +0.3 339,184 - +0.0 339,195 + +0.5 339,195 + +0.4 339,203 - -0.3 339,203 - +0.0 339,378 - +0.4 339,390 - +0.9 339,390 - +0.2 339,411 - -0.5 339,431 + +0.0 339,444 - -0.7 339,565 + yahI b0323 0.19 +2.0 339,565 + yahI b0323 0.19 +2.0 339,630 + yahI b0323 0.25 +1.4 339,630 + yahI b0323 0.25 +1.8 339,741 - yahI b0323 0.37 +0.3 339,798 + yahI b0323 0.43 +0.2 339,806 - yahI b0323 0.44 +0.4 340,025 + yahI b0323 0.67 +0.0 340,025 + yahI b0323 0.67 -0.9 340,047 - yahI b0323 0.69 -0.1 340,170 - yahI b0323 0.82 +0.5 340,174 - yahI b0323 0.83 +0.7 340,174 - yahI b0323 0.83 +1.0 340,257 + +1.7 340,257 + +0.8 340,320 + +1.0 340,320 + +0.4 340,394 + +0.4 340,448 + +0.0 340,461 + -1.4 340,522 - yahJ b0324 0.13 +1.0 340,573 - yahJ b0324 0.16 -1.6 340,573 - yahJ b0324 0.16 -0.2 340,675 + yahJ b0324 0.24 +0.3 340,675 + yahJ b0324 0.24 +0.8 340,776 + yahJ b0324 0.31 +0.8 340,952 + yahJ b0324 0.44 -0.7 341,087 - yahJ b0324 0.53 -0.5 341,087 - yahJ b0324 0.53 -1.2 341,124 + yahJ b0324 0.56 +0.4 341,139 - yahJ b0324 0.57 -0.1 341,141 + yahJ b0324 0.57 +0.8
Or see this region's nucleotide sequence