Experiment: LB Anaerobic with Ciprofloxacin Hydrochloride 0.00000001953125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yobB and exoX are separated by 23 nucleotides exoX and ptrB overlap by 4 nucleotides ptrB and yebE are separated by 208 nucleotides
b1843: yobB - hypothetical protein (NCBI), at 1,923,464 to 1,924,120
yobB
b1844: exoX - exodeoxyribonuclease X (NCBI), at 1,924,144 to 1,924,806
exoX
b1845: ptrB - protease II (NCBI), at 1,924,803 to 1,926,863
ptrB
b1846: yebE - hypothetical protein (NCBI), at 1,927,072 to 1,927,731
yebE
Position (kb)
1924
1925
1926
1927 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1923.831 kb on - strand, within yobB at 1923.840 kb on - strand, within yobB at 1923.840 kb on - strand, within yobB at 1923.846 kb on + strand, within yobB at 1923.995 kb on + strand, within yobB at 1924.003 kb on - strand, within yobB at 1924.274 kb on + strand, within exoX at 1924.274 kb on + strand, within exoX at 1924.274 kb on - strand, within exoX at 1924.309 kb on - strand, within exoX at 1924.361 kb on - strand, within exoX at 1924.361 kb on - strand, within exoX at 1924.423 kb on + strand, within exoX at 1924.423 kb on + strand, within exoX at 1924.471 kb on + strand, within exoX at 1924.507 kb on - strand, within exoX at 1924.609 kb on - strand, within exoX at 1924.715 kb on + strand, within exoX at 1924.770 kb on + strand at 1924.831 kb on - strand at 1924.893 kb on + strand at 1925.014 kb on - strand, within ptrB at 1925.128 kb on + strand, within ptrB at 1925.128 kb on + strand, within ptrB at 1925.602 kb on + strand, within ptrB at 1925.602 kb on + strand, within ptrB at 1925.668 kb on - strand, within ptrB at 1925.757 kb on - strand, within ptrB at 1925.785 kb on - strand, within ptrB at 1925.976 kb on + strand, within ptrB at 1925.976 kb on + strand, within ptrB at 1925.976 kb on + strand, within ptrB at 1925.984 kb on - strand, within ptrB at 1925.984 kb on - strand, within ptrB at 1926.042 kb on + strand, within ptrB at 1926.048 kb on + strand, within ptrB at 1926.056 kb on - strand, within ptrB at 1926.172 kb on + strand, within ptrB at 1926.172 kb on + strand, within ptrB at 1926.326 kb on - strand, within ptrB at 1926.403 kb on - strand, within ptrB at 1926.489 kb on - strand, within ptrB at 1926.492 kb on + strand, within ptrB at 1926.492 kb on + strand, within ptrB at 1926.531 kb on + strand, within ptrB at 1926.554 kb on - strand, within ptrB at 1926.554 kb on - strand, within ptrB at 1926.593 kb on + strand, within ptrB at 1926.750 kb on + strand at 1926.750 kb on + strand at 1927.234 kb on + strand, within yebE at 1927.234 kb on + strand, within yebE at 1927.267 kb on + strand, within yebE at 1927.334 kb on - strand, within yebE at 1927.382 kb on - strand, within yebE at 1927.382 kb on - strand, within yebE at 1927.407 kb on + strand, within yebE at 1927.533 kb on + strand, within yebE at 1927.578 kb on + strand, within yebE at 1927.767 kb on - strand at 1927.861 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Ciprofloxacin Hydrochloride 0.00000001953125 mM remove 1,923,831 - yobB b1843 0.56 -0.1 1,923,840 - yobB b1843 0.57 -0.1 1,923,840 - yobB b1843 0.57 -0.5 1,923,846 + yobB b1843 0.58 -0.6 1,923,995 + yobB b1843 0.81 +0.0 1,924,003 - yobB b1843 0.82 -0.0 1,924,274 + exoX b1844 0.20 +0.6 1,924,274 + exoX b1844 0.20 -0.1 1,924,274 - exoX b1844 0.20 -0.1 1,924,309 - exoX b1844 0.25 -1.5 1,924,361 - exoX b1844 0.33 +0.5 1,924,361 - exoX b1844 0.33 +0.5 1,924,423 + exoX b1844 0.42 -0.2 1,924,423 + exoX b1844 0.42 -0.3 1,924,471 + exoX b1844 0.49 +0.7 1,924,507 - exoX b1844 0.55 +0.5 1,924,609 - exoX b1844 0.70 -1.1 1,924,715 + exoX b1844 0.86 +0.4 1,924,770 + -2.3 1,924,831 - +0.4 1,924,893 + +0.2 1,925,014 - ptrB b1845 0.10 +1.1 1,925,128 + ptrB b1845 0.16 -0.1 1,925,128 + ptrB b1845 0.16 +0.5 1,925,602 + ptrB b1845 0.39 +0.6 1,925,602 + ptrB b1845 0.39 -0.2 1,925,668 - ptrB b1845 0.42 +0.4 1,925,757 - ptrB b1845 0.46 -0.1 1,925,785 - ptrB b1845 0.48 -0.2 1,925,976 + ptrB b1845 0.57 +1.0 1,925,976 + ptrB b1845 0.57 -0.0 1,925,976 + ptrB b1845 0.57 -1.1 1,925,984 - ptrB b1845 0.57 +0.0 1,925,984 - ptrB b1845 0.57 +0.0 1,926,042 + ptrB b1845 0.60 +0.5 1,926,048 + ptrB b1845 0.60 +0.8 1,926,056 - ptrB b1845 0.61 +0.7 1,926,172 + ptrB b1845 0.66 +1.2 1,926,172 + ptrB b1845 0.66 +0.5 1,926,326 - ptrB b1845 0.74 -0.1 1,926,403 - ptrB b1845 0.78 +0.7 1,926,489 - ptrB b1845 0.82 +0.1 1,926,492 + ptrB b1845 0.82 +0.3 1,926,492 + ptrB b1845 0.82 -0.8 1,926,531 + ptrB b1845 0.84 +0.9 1,926,554 - ptrB b1845 0.85 +0.3 1,926,554 - ptrB b1845 0.85 -0.2 1,926,593 + ptrB b1845 0.87 -0.1 1,926,750 + +0.6 1,926,750 + +0.1 1,927,234 + yebE b1846 0.25 +2.5 1,927,234 + yebE b1846 0.25 +0.6 1,927,267 + yebE b1846 0.30 -0.2 1,927,334 - yebE b1846 0.40 +0.2 1,927,382 - yebE b1846 0.47 -0.5 1,927,382 - yebE b1846 0.47 +1.4 1,927,407 + yebE b1846 0.51 +0.7 1,927,533 + yebE b1846 0.70 +0.6 1,927,578 + yebE b1846 0.77 +0.5 1,927,767 - +0.1 1,927,861 + -0.3
Or see this region's nucleotide sequence