Strain Fitness in Escherichia coli BW25113 around b1190

Experiment: LB Anaerobic with Ciprofloxacin Hydrochloride 0.00000001953125 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntdadA and dadX are separated by 9 nucleotidesdadX and b1191 are separated by 385 nucleotides b1189: dadA - D-amino acid dehydrogenase (quinone) DadA (EC 1.4.5.1) (from data), at 1,236,794 to 1,238,092 dadA b1190: dadX - alanine racemase (NCBI), at 1,238,102 to 1,239,172 dadX b1191: b1191 - orf, hypothetical protein (VIMSS), at 1,239,558 to 1,241,294 b1191 Position (kb) 1238 1239 1240Strain fitness (log2 ratio) -1 0 1at 1237.146 kb on + strand, within dadAat 1237.180 kb on + strand, within dadAat 1237.213 kb on + strand, within dadAat 1237.213 kb on + strand, within dadAat 1237.218 kb on + strand, within dadAat 1237.507 kb on + strand, within dadAat 1237.545 kb on - strand, within dadAat 1237.545 kb on - strand, within dadAat 1237.803 kb on + strand, within dadAat 1237.803 kb on + strand, within dadAat 1238.119 kb on + strandat 1238.119 kb on + strandat 1238.234 kb on - strand, within dadXat 1238.234 kb on - strand, within dadXat 1238.275 kb on + strand, within dadXat 1238.275 kb on + strand, within dadXat 1238.292 kb on + strand, within dadXat 1238.803 kb on + strand, within dadXat 1238.803 kb on + strand, within dadXat 1238.855 kb on + strand, within dadXat 1238.874 kb on - strand, within dadXat 1238.920 kb on - strand, within dadXat 1238.920 kb on - strand, within dadXat 1239.281 kb on - strandat 1239.422 kb on - strandat 1239.639 kb on - strandat 1239.674 kb on + strandat 1239.674 kb on + strandat 1239.690 kb on - strandat 1239.769 kb on + strand, within b1191at 1239.842 kb on + strand, within b1191at 1239.886 kb on + strand, within b1191at 1239.886 kb on + strand, within b1191at 1239.977 kb on + strand, within b1191

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Anaerobic with Ciprofloxacin Hydrochloride 0.00000001953125 mM
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1,237,146 + dadA b1189 0.27 -0.2
1,237,180 + dadA b1189 0.30 -0.6
1,237,213 + dadA b1189 0.32 -0.9
1,237,213 + dadA b1189 0.32 -0.7
1,237,218 + dadA b1189 0.33 +0.8
1,237,507 + dadA b1189 0.55 +0.1
1,237,545 - dadA b1189 0.58 -1.1
1,237,545 - dadA b1189 0.58 +0.3
1,237,803 + dadA b1189 0.78 +0.2
1,237,803 + dadA b1189 0.78 +0.4
1,238,119 + -0.1
1,238,119 + -0.1
1,238,234 - dadX b1190 0.12 -0.2
1,238,234 - dadX b1190 0.12 +0.7
1,238,275 + dadX b1190 0.16 -0.9
1,238,275 + dadX b1190 0.16 +0.5
1,238,292 + dadX b1190 0.18 -0.3
1,238,803 + dadX b1190 0.65 +0.1
1,238,803 + dadX b1190 0.65 -0.2
1,238,855 + dadX b1190 0.70 -0.2
1,238,874 - dadX b1190 0.72 -0.3
1,238,920 - dadX b1190 0.76 +0.7
1,238,920 - dadX b1190 0.76 +1.7
1,239,281 - -0.6
1,239,422 - -0.1
1,239,639 - +1.7
1,239,674 + -0.7
1,239,674 + -0.5
1,239,690 - +1.7
1,239,769 + b1191 b1191 0.12 +0.2
1,239,842 + b1191 b1191 0.16 -0.2
1,239,886 + b1191 b1191 0.19 +0.4
1,239,886 + b1191 b1191 0.19 +0.1
1,239,977 + b1191 b1191 0.24 +0.2

Or see this region's nucleotide sequence