Experiment: LB Anaerobic with Ciprofloxacin Hydrochloride 0.0000000390625 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt fucU and fucR are separated by 57 nucleotides fucR and ygdE are separated by 43 nucleotides ygdE and ygdD overlap by 8 nucleotides ygdD and gcvA are separated by 18 nucleotides
b2804: fucU - L-fucose mutarotase (NCBI), at 2,936,910 to 2,937,332
fucU
b2805: fucR - DNA-binding transcriptional activator (NCBI), at 2,937,390 to 2,938,121
fucR
b2806: ygdE - predicted methyltransferase (NCBI), at 2,938,165 to 2,939,265
ygdE
b2807: ygdD - conserved inner membrane protein (NCBI), at 2,939,258 to 2,939,653
ygdD
b2808: gcvA - DNA-binding transcriptional dual regulator (NCBI), at 2,939,672 to 2,940,589
gcvA
Position (kb)
2938
2939
2940 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2937.173 kb on + strand, within fucU at 2937.434 kb on - strand at 2937.434 kb on - strand at 2937.439 kb on - strand at 2937.473 kb on - strand, within fucR at 2937.629 kb on + strand, within fucR at 2937.677 kb on - strand, within fucR at 2937.677 kb on - strand, within fucR at 2937.681 kb on + strand, within fucR at 2937.747 kb on - strand, within fucR at 2937.769 kb on + strand, within fucR at 2937.769 kb on + strand, within fucR at 2937.811 kb on - strand, within fucR at 2937.811 kb on - strand, within fucR at 2937.817 kb on + strand, within fucR at 2937.862 kb on + strand, within fucR at 2938.088 kb on + strand at 2938.132 kb on - strand at 2938.204 kb on - strand at 2938.353 kb on - strand, within ygdE at 2938.353 kb on - strand, within ygdE at 2938.398 kb on - strand, within ygdE at 2938.443 kb on + strand, within ygdE at 2938.443 kb on - strand, within ygdE at 2938.553 kb on + strand, within ygdE at 2938.561 kb on - strand, within ygdE at 2938.561 kb on - strand, within ygdE at 2938.587 kb on + strand, within ygdE at 2938.610 kb on + strand, within ygdE at 2938.698 kb on + strand, within ygdE at 2938.711 kb on - strand, within ygdE at 2938.711 kb on - strand, within ygdE at 2938.750 kb on - strand, within ygdE at 2938.758 kb on + strand, within ygdE at 2938.780 kb on - strand, within ygdE at 2938.780 kb on - strand, within ygdE at 2938.795 kb on - strand, within ygdE at 2938.795 kb on - strand, within ygdE at 2938.810 kb on + strand, within ygdE at 2938.810 kb on + strand, within ygdE at 2938.868 kb on - strand, within ygdE at 2938.883 kb on - strand, within ygdE at 2938.969 kb on + strand, within ygdE at 2938.974 kb on + strand, within ygdE at 2938.974 kb on + strand, within ygdE at 2938.986 kb on + strand, within ygdE at 2939.038 kb on - strand, within ygdE at 2939.298 kb on - strand, within ygdD at 2939.347 kb on + strand, within ygdD at 2939.405 kb on + strand, within ygdD at 2939.442 kb on - strand, within ygdD at 2939.465 kb on - strand, within ygdD at 2939.470 kb on + strand, within ygdD at 2939.470 kb on + strand, within ygdD at 2939.545 kb on + strand, within ygdD at 2939.553 kb on - strand, within ygdD at 2939.648 kb on + strand at 2939.648 kb on + strand at 2939.655 kb on + strand at 2939.663 kb on - strand at 2939.663 kb on - strand at 2939.778 kb on - strand, within gcvA at 2939.778 kb on - strand, within gcvA at 2939.804 kb on + strand, within gcvA at 2939.912 kb on - strand, within gcvA at 2939.914 kb on - strand, within gcvA at 2939.982 kb on - strand, within gcvA at 2939.982 kb on - strand, within gcvA at 2939.985 kb on - strand, within gcvA at 2939.987 kb on - strand, within gcvA at 2940.001 kb on + strand, within gcvA at 2940.068 kb on + strand, within gcvA at 2940.171 kb on - strand, within gcvA at 2940.171 kb on - strand, within gcvA
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Ciprofloxacin Hydrochloride 0.0000000390625 mM remove 2,937,173 + fucU b2804 0.62 -1.2 2,937,434 - +1.8 2,937,434 - -1.0 2,937,439 - -0.6 2,937,473 - fucR b2805 0.11 -1.2 2,937,629 + fucR b2805 0.33 -0.3 2,937,677 - fucR b2805 0.39 +0.1 2,937,677 - fucR b2805 0.39 -0.6 2,937,681 + fucR b2805 0.40 -1.6 2,937,747 - fucR b2805 0.49 -0.6 2,937,769 + fucR b2805 0.52 +1.8 2,937,769 + fucR b2805 0.52 -3.4 2,937,811 - fucR b2805 0.58 +0.3 2,937,811 - fucR b2805 0.58 +0.1 2,937,817 + fucR b2805 0.58 -1.7 2,937,862 + fucR b2805 0.64 -0.1 2,938,088 + -0.6 2,938,132 - +1.0 2,938,204 - +1.0 2,938,353 - ygdE b2806 0.17 -1.2 2,938,353 - ygdE b2806 0.17 -0.4 2,938,398 - ygdE b2806 0.21 -0.4 2,938,443 + ygdE b2806 0.25 +1.0 2,938,443 - ygdE b2806 0.25 +0.4 2,938,553 + ygdE b2806 0.35 -0.3 2,938,561 - ygdE b2806 0.36 +1.2 2,938,561 - ygdE b2806 0.36 -0.5 2,938,587 + ygdE b2806 0.38 -0.6 2,938,610 + ygdE b2806 0.40 -0.2 2,938,698 + ygdE b2806 0.48 -0.8 2,938,711 - ygdE b2806 0.50 -0.7 2,938,711 - ygdE b2806 0.50 +0.8 2,938,750 - ygdE b2806 0.53 -0.7 2,938,758 + ygdE b2806 0.54 -1.5 2,938,780 - ygdE b2806 0.56 -1.5 2,938,780 - ygdE b2806 0.56 +0.1 2,938,795 - ygdE b2806 0.57 -0.3 2,938,795 - ygdE b2806 0.57 -0.4 2,938,810 + ygdE b2806 0.59 -0.2 2,938,810 + ygdE b2806 0.59 -0.3 2,938,868 - ygdE b2806 0.64 -0.7 2,938,883 - ygdE b2806 0.65 -0.2 2,938,969 + ygdE b2806 0.73 -0.0 2,938,974 + ygdE b2806 0.73 -1.2 2,938,974 + ygdE b2806 0.73 -1.0 2,938,986 + ygdE b2806 0.75 -0.7 2,939,038 - ygdE b2806 0.79 -1.1 2,939,298 - ygdD b2807 0.10 -2.3 2,939,347 + ygdD b2807 0.22 -0.6 2,939,405 + ygdD b2807 0.37 -0.3 2,939,442 - ygdD b2807 0.46 -2.2 2,939,465 - ygdD b2807 0.52 -0.0 2,939,470 + ygdD b2807 0.54 -1.5 2,939,470 + ygdD b2807 0.54 -1.5 2,939,545 + ygdD b2807 0.72 -0.6 2,939,553 - ygdD b2807 0.74 -2.9 2,939,648 + -2.5 2,939,648 + -3.3 2,939,655 + -1.4 2,939,663 - -0.0 2,939,663 - -0.7 2,939,778 - gcvA b2808 0.12 +1.2 2,939,778 - gcvA b2808 0.12 +0.2 2,939,804 + gcvA b2808 0.14 -0.0 2,939,912 - gcvA b2808 0.26 +2.6 2,939,914 - gcvA b2808 0.26 +1.0 2,939,982 - gcvA b2808 0.34 +2.6 2,939,982 - gcvA b2808 0.34 +1.0 2,939,985 - gcvA b2808 0.34 +1.3 2,939,987 - gcvA b2808 0.34 +1.7 2,940,001 + gcvA b2808 0.36 +2.0 2,940,068 + gcvA b2808 0.43 +1.3 2,940,171 - gcvA b2808 0.54 +1.6 2,940,171 - gcvA b2808 0.54 +1.0
Or see this region's nucleotide sequence