Strain Fitness in Escherichia coli BW25113 around b0473

Experiment: LB Anaerobic with Ciprofloxacin Hydrochloride 0.0000000390625 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntybaB and recR overlap by 1 nucleotidesrecR and htpG are separated by 109 nucleotideshtpG and adk are separated by 180 nucleotides b0471: ybaB - hypothetical protein (NCBI), at 493,300 to 493,629 ybaB b0472: recR - recombination protein RecR (NCBI), at 493,629 to 494,234 recR b0473: htpG - heat shock protein 90 (NCBI), at 494,344 to 496,218 htpG b0474: adk - adenylate kinase (NCBI), at 496,399 to 497,043 adk Position (kb) 494 495 496 497Strain fitness (log2 ratio) -3 -2 -1 0 1at 493.392 kb on + strand, within ybaBat 493.488 kb on - strand, within ybaBat 493.499 kb on - strand, within ybaBat 493.499 kb on - strand, within ybaBat 493.517 kb on + strand, within ybaBat 493.713 kb on - strand, within recRat 493.724 kb on + strand, within recRat 493.879 kb on + strand, within recRat 493.882 kb on - strand, within recRat 493.887 kb on - strand, within recRat 493.887 kb on - strand, within recRat 493.949 kb on + strand, within recRat 493.993 kb on + strand, within recRat 494.016 kb on - strand, within recRat 494.016 kb on - strand, within recRat 494.016 kb on - strand, within recRat 494.018 kb on + strand, within recRat 494.057 kb on + strand, within recRat 494.074 kb on + strand, within recRat 494.094 kb on - strand, within recRat 494.099 kb on - strand, within recRat 494.115 kb on + strand, within recRat 494.211 kb on - strandat 494.211 kb on - strandat 494.211 kb on - strandat 494.291 kb on - strandat 494.345 kb on - strandat 494.345 kb on - strandat 494.448 kb on + strandat 494.517 kb on - strandat 494.561 kb on + strand, within htpGat 494.596 kb on - strand, within htpGat 494.619 kb on - strand, within htpGat 494.630 kb on - strand, within htpGat 494.658 kb on + strand, within htpGat 494.758 kb on + strand, within htpGat 494.821 kb on + strand, within htpGat 494.871 kb on - strand, within htpGat 494.871 kb on - strand, within htpGat 495.048 kb on - strand, within htpGat 495.108 kb on + strand, within htpGat 495.133 kb on + strand, within htpGat 495.155 kb on + strand, within htpGat 495.160 kb on + strand, within htpGat 495.164 kb on + strand, within htpGat 495.174 kb on + strand, within htpGat 495.205 kb on + strand, within htpGat 495.249 kb on + strand, within htpGat 495.255 kb on + strand, within htpGat 495.267 kb on - strand, within htpGat 495.267 kb on - strand, within htpGat 495.269 kb on + strand, within htpGat 495.269 kb on + strand, within htpGat 495.277 kb on - strand, within htpGat 495.277 kb on - strand, within htpGat 495.292 kb on + strand, within htpGat 495.377 kb on + strand, within htpGat 495.377 kb on + strand, within htpGat 495.497 kb on - strand, within htpGat 495.526 kb on - strand, within htpGat 495.622 kb on - strand, within htpGat 495.643 kb on + strand, within htpGat 495.643 kb on + strand, within htpGat 495.651 kb on - strand, within htpGat 495.653 kb on + strand, within htpGat 495.661 kb on + strand, within htpGat 495.661 kb on + strand, within htpGat 495.763 kb on - strand, within htpGat 495.783 kb on - strand, within htpGat 495.968 kb on + strand, within htpGat 496.118 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Anaerobic with Ciprofloxacin Hydrochloride 0.0000000390625 mM
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493,392 + ybaB b0471 0.28 +0.1
493,488 - ybaB b0471 0.57 -1.1
493,499 - ybaB b0471 0.60 +1.2
493,499 - ybaB b0471 0.60 -0.5
493,517 + ybaB b0471 0.66 -2.7
493,713 - recR b0472 0.14 -1.6
493,724 + recR b0472 0.16 -2.2
493,879 + recR b0472 0.41 -1.0
493,882 - recR b0472 0.42 -0.8
493,887 - recR b0472 0.43 -1.5
493,887 - recR b0472 0.43 +1.2
493,949 + recR b0472 0.53 -0.5
493,993 + recR b0472 0.60 -2.4
494,016 - recR b0472 0.64 -0.6
494,016 - recR b0472 0.64 -2.0
494,016 - recR b0472 0.64 -0.2
494,018 + recR b0472 0.64 -0.8
494,057 + recR b0472 0.71 -0.5
494,074 + recR b0472 0.73 -1.1
494,094 - recR b0472 0.77 -0.1
494,099 - recR b0472 0.78 +0.8
494,115 + recR b0472 0.80 -1.1
494,211 - -1.8
494,211 - -0.4
494,211 - -1.8
494,291 - -0.4
494,345 - -0.8
494,345 - +1.6
494,448 + -1.9
494,517 - +0.0
494,561 + htpG b0473 0.12 -1.0
494,596 - htpG b0473 0.13 -1.0
494,619 - htpG b0473 0.15 -1.1
494,630 - htpG b0473 0.15 -1.4
494,658 + htpG b0473 0.17 -1.4
494,758 + htpG b0473 0.22 +0.3
494,821 + htpG b0473 0.25 -0.8
494,871 - htpG b0473 0.28 -1.4
494,871 - htpG b0473 0.28 -1.1
495,048 - htpG b0473 0.38 -0.9
495,108 + htpG b0473 0.41 -2.4
495,133 + htpG b0473 0.42 -0.4
495,155 + htpG b0473 0.43 -3.1
495,160 + htpG b0473 0.44 -2.4
495,164 + htpG b0473 0.44 -2.8
495,174 + htpG b0473 0.44 -3.1
495,205 + htpG b0473 0.46 -2.7
495,249 + htpG b0473 0.48 -1.7
495,255 + htpG b0473 0.49 -0.6
495,267 - htpG b0473 0.49 -1.0
495,267 - htpG b0473 0.49 +0.9
495,269 + htpG b0473 0.49 -1.1
495,269 + htpG b0473 0.49 -0.1
495,277 - htpG b0473 0.50 -1.0
495,277 - htpG b0473 0.50 -0.6
495,292 + htpG b0473 0.51 -1.4
495,377 + htpG b0473 0.55 -1.0
495,377 + htpG b0473 0.55 -0.1
495,497 - htpG b0473 0.61 +0.3
495,526 - htpG b0473 0.63 -0.4
495,622 - htpG b0473 0.68 +1.0
495,643 + htpG b0473 0.69 -2.1
495,643 + htpG b0473 0.69 -0.4
495,651 - htpG b0473 0.70 +0.1
495,653 + htpG b0473 0.70 +0.0
495,661 + htpG b0473 0.70 -2.3
495,661 + htpG b0473 0.70 -1.4
495,763 - htpG b0473 0.76 -0.9
495,783 - htpG b0473 0.77 -2.5
495,968 + htpG b0473 0.87 -1.2
496,118 + +1.1

Or see this region's nucleotide sequence