Strain Fitness in Escherichia coli BW25113 around b4119

Experiment: LB Anaerobic with Novobiocin 0.00003125 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntmelR and melA are separated by 282 nucleotidesmelA and melB are separated by 102 nucleotides b4118: melR - DNA-binding transcriptional dual regulator (NCBI), at 4,338,743 to 4,339,651 melR b4119: melA - alpha-galactosidase, NAD(P)-binding (NCBI), at 4,339,934 to 4,341,289 melA b4120: melB - melibiose:sodium symporter (NCBI), at 4,341,392 to 4,342,813 melB Position (kb) 4339 4340 4341 4342Strain fitness (log2 ratio) -2 -1 0 1 2at 4339.019 kb on + strand, within melRat 4339.030 kb on + strand, within melRat 4339.030 kb on + strand, within melRat 4339.346 kb on - strand, within melRat 4339.376 kb on + strand, within melRat 4339.376 kb on + strand, within melRat 4339.421 kb on - strand, within melRat 4339.427 kb on - strand, within melRat 4339.434 kb on + strand, within melRat 4339.501 kb on + strand, within melRat 4339.549 kb on - strand, within melRat 4339.625 kb on + strandat 4339.644 kb on + strandat 4339.700 kb on + strandat 4339.700 kb on + strandat 4339.700 kb on - strandat 4339.700 kb on - strandat 4339.734 kb on + strandat 4339.744 kb on - strandat 4339.744 kb on - strandat 4339.755 kb on - strandat 4339.762 kb on + strandat 4340.006 kb on + strandat 4340.060 kb on - strandat 4340.156 kb on + strand, within melAat 4340.257 kb on - strand, within melAat 4340.259 kb on + strand, within melAat 4340.282 kb on - strand, within melAat 4340.297 kb on - strand, within melAat 4340.297 kb on - strand, within melAat 4340.297 kb on - strand, within melAat 4340.303 kb on + strand, within melAat 4340.305 kb on + strand, within melAat 4340.307 kb on + strand, within melAat 4340.350 kb on + strand, within melAat 4340.363 kb on - strand, within melAat 4340.379 kb on + strand, within melAat 4340.413 kb on - strand, within melAat 4340.413 kb on - strand, within melAat 4340.430 kb on + strand, within melAat 4340.440 kb on + strand, within melAat 4340.452 kb on - strand, within melAat 4340.452 kb on - strand, within melAat 4340.457 kb on - strand, within melAat 4340.457 kb on - strand, within melAat 4340.746 kb on + strand, within melAat 4340.746 kb on + strand, within melAat 4340.746 kb on + strand, within melAat 4340.754 kb on - strand, within melAat 4340.765 kb on + strand, within melAat 4340.765 kb on + strand, within melAat 4340.799 kb on - strand, within melAat 4340.869 kb on + strand, within melAat 4340.917 kb on - strand, within melAat 4340.924 kb on + strand, within melAat 4340.934 kb on - strand, within melAat 4340.944 kb on - strand, within melAat 4340.944 kb on - strand, within melAat 4340.981 kb on - strand, within melAat 4341.084 kb on - strand, within melAat 4341.084 kb on - strand, within melAat 4341.111 kb on - strand, within melAat 4341.133 kb on - strand, within melAat 4341.176 kb on + strandat 4341.176 kb on - strandat 4341.211 kb on - strandat 4341.211 kb on - strandat 4341.230 kb on + strandat 4341.262 kb on - strandat 4341.293 kb on - strandat 4341.293 kb on - strandat 4341.296 kb on + strandat 4341.329 kb on + strandat 4341.380 kb on + strandat 4341.396 kb on - strandat 4341.464 kb on + strandat 4341.472 kb on - strandat 4341.477 kb on - strandat 4341.479 kb on + strandat 4341.484 kb on + strandat 4341.508 kb on + strandat 4341.508 kb on + strandat 4341.518 kb on - strandat 4341.518 kb on - strandat 4341.528 kb on + strandat 4341.538 kb on + strand, within melBat 4341.538 kb on + strand, within melBat 4341.641 kb on + strand, within melBat 4341.670 kb on + strand, within melBat 4341.688 kb on + strand, within melBat 4341.688 kb on + strand, within melBat 4341.696 kb on + strand, within melBat 4341.696 kb on + strand, within melBat 4341.704 kb on - strand, within melBat 4341.704 kb on - strand, within melBat 4341.724 kb on + strand, within melBat 4341.763 kb on - strand, within melBat 4341.786 kb on + strand, within melBat 4341.809 kb on - strand, within melBat 4341.939 kb on + strand, within melBat 4341.939 kb on + strand, within melBat 4341.939 kb on + strand, within melBat 4341.939 kb on + strand, within melBat 4341.951 kb on - strand, within melBat 4341.951 kb on - strand, within melBat 4341.975 kb on + strand, within melBat 4341.975 kb on + strand, within melBat 4341.979 kb on - strand, within melBat 4341.980 kb on + strand, within melBat 4341.981 kb on - strand, within melBat 4341.983 kb on - strand, within melBat 4341.985 kb on + strand, within melBat 4341.985 kb on + strand, within melBat 4341.990 kb on + strand, within melBat 4341.990 kb on + strand, within melBat 4342.014 kb on + strand, within melBat 4342.028 kb on + strand, within melBat 4342.039 kb on - strand, within melBat 4342.041 kb on + strand, within melBat 4342.041 kb on + strand, within melBat 4342.055 kb on - strand, within melBat 4342.055 kb on - strand, within melBat 4342.104 kb on - strand, within melBat 4342.179 kb on - strand, within melBat 4342.187 kb on + strand, within melBat 4342.200 kb on + strand, within melBat 4342.200 kb on + strand, within melBat 4342.202 kb on + strand, within melBat 4342.202 kb on + strand, within melBat 4342.215 kb on - strand, within melBat 4342.218 kb on - strand, within melBat 4342.220 kb on + strand, within melBat 4342.228 kb on - strand, within melBat 4342.228 kb on - strand, within melBat 4342.244 kb on - strand, within melBat 4342.261 kb on - strand, within melBat 4342.265 kb on - strand, within melBat 4342.283 kb on + strand, within melB

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Anaerobic with Novobiocin 0.00003125 mM
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4,339,019 + melR b4118 0.30 +0.1
4,339,030 + melR b4118 0.32 +0.8
4,339,030 + melR b4118 0.32 -1.1
4,339,346 - melR b4118 0.66 -0.1
4,339,376 + melR b4118 0.70 -0.2
4,339,376 + melR b4118 0.70 -0.1
4,339,421 - melR b4118 0.75 -0.7
4,339,427 - melR b4118 0.75 +0.1
4,339,434 + melR b4118 0.76 -0.3
4,339,501 + melR b4118 0.83 -0.3
4,339,549 - melR b4118 0.89 -0.3
4,339,625 + -0.4
4,339,644 + +0.1
4,339,700 + -0.5
4,339,700 + +0.2
4,339,700 - +0.6
4,339,700 - +0.4
4,339,734 + -1.8
4,339,744 - +0.6
4,339,744 - +1.4
4,339,755 - +0.4
4,339,762 + +0.2
4,340,006 + +0.1
4,340,060 - -0.7
4,340,156 + melA b4119 0.16 +0.4
4,340,257 - melA b4119 0.24 +0.4
4,340,259 + melA b4119 0.24 -0.4
4,340,282 - melA b4119 0.26 -0.6
4,340,297 - melA b4119 0.27 -0.2
4,340,297 - melA b4119 0.27 +0.3
4,340,297 - melA b4119 0.27 -0.1
4,340,303 + melA b4119 0.27 -0.5
4,340,305 + melA b4119 0.27 -0.9
4,340,307 + melA b4119 0.28 +0.3
4,340,350 + melA b4119 0.31 +0.7
4,340,363 - melA b4119 0.32 -0.2
4,340,379 + melA b4119 0.33 +0.2
4,340,413 - melA b4119 0.35 +0.7
4,340,413 - melA b4119 0.35 +0.4
4,340,430 + melA b4119 0.37 +0.6
4,340,440 + melA b4119 0.37 -1.3
4,340,452 - melA b4119 0.38 -0.2
4,340,452 - melA b4119 0.38 +0.2
4,340,457 - melA b4119 0.39 +0.1
4,340,457 - melA b4119 0.39 -0.0
4,340,746 + melA b4119 0.60 +0.3
4,340,746 + melA b4119 0.60 +0.2
4,340,746 + melA b4119 0.60 +0.2
4,340,754 - melA b4119 0.60 +0.3
4,340,765 + melA b4119 0.61 +0.1
4,340,765 + melA b4119 0.61 -0.4
4,340,799 - melA b4119 0.64 +1.0
4,340,869 + melA b4119 0.69 -1.2
4,340,917 - melA b4119 0.72 +0.1
4,340,924 + melA b4119 0.73 +0.6
4,340,934 - melA b4119 0.74 +0.1
4,340,944 - melA b4119 0.74 +0.4
4,340,944 - melA b4119 0.74 +0.9
4,340,981 - melA b4119 0.77 +0.3
4,341,084 - melA b4119 0.85 +0.3
4,341,084 - melA b4119 0.85 -0.2
4,341,111 - melA b4119 0.87 -0.6
4,341,133 - melA b4119 0.88 +1.3
4,341,176 + -0.8
4,341,176 - -1.3
4,341,211 - -0.4
4,341,211 - +0.8
4,341,230 + -0.4
4,341,262 - -0.7
4,341,293 - -0.4
4,341,293 - -0.1
4,341,296 + -0.9
4,341,329 + -1.0
4,341,380 + +1.7
4,341,396 - +0.8
4,341,464 + -0.4
4,341,472 - -1.3
4,341,477 - -0.6
4,341,479 + +0.4
4,341,484 + +0.3
4,341,508 + +1.6
4,341,508 + -0.3
4,341,518 - +0.2
4,341,518 - -0.3
4,341,528 + +0.3
4,341,538 + melB b4120 0.10 +0.4
4,341,538 + melB b4120 0.10 -0.3
4,341,641 + melB b4120 0.18 -0.0
4,341,670 + melB b4120 0.20 -1.7
4,341,688 + melB b4120 0.21 -0.2
4,341,688 + melB b4120 0.21 -0.3
4,341,696 + melB b4120 0.21 +0.6
4,341,696 + melB b4120 0.21 -1.3
4,341,704 - melB b4120 0.22 +0.5
4,341,704 - melB b4120 0.22 +0.1
4,341,724 + melB b4120 0.23 -0.7
4,341,763 - melB b4120 0.26 -0.2
4,341,786 + melB b4120 0.28 -0.4
4,341,809 - melB b4120 0.29 -0.1
4,341,939 + melB b4120 0.38 -1.0
4,341,939 + melB b4120 0.38 -0.2
4,341,939 + melB b4120 0.38 -2.3
4,341,939 + melB b4120 0.38 -0.1
4,341,951 - melB b4120 0.39 -0.6
4,341,951 - melB b4120 0.39 -1.8
4,341,975 + melB b4120 0.41 -0.1
4,341,975 + melB b4120 0.41 -0.8
4,341,979 - melB b4120 0.41 -0.3
4,341,980 + melB b4120 0.41 -0.2
4,341,981 - melB b4120 0.41 +2.6
4,341,983 - melB b4120 0.42 +0.6
4,341,985 + melB b4120 0.42 +0.1
4,341,985 + melB b4120 0.42 -0.3
4,341,990 + melB b4120 0.42 +0.4
4,341,990 + melB b4120 0.42 +0.7
4,342,014 + melB b4120 0.44 -0.0
4,342,028 + melB b4120 0.45 -0.9
4,342,039 - melB b4120 0.45 -0.5
4,342,041 + melB b4120 0.46 +0.4
4,342,041 + melB b4120 0.46 +0.1
4,342,055 - melB b4120 0.47 +0.7
4,342,055 - melB b4120 0.47 +0.3
4,342,104 - melB b4120 0.50 +2.0
4,342,179 - melB b4120 0.55 -0.0
4,342,187 + melB b4120 0.56 +0.5
4,342,200 + melB b4120 0.57 -0.8
4,342,200 + melB b4120 0.57 -0.6
4,342,202 + melB b4120 0.57 -1.3
4,342,202 + melB b4120 0.57 -2.1
4,342,215 - melB b4120 0.58 +0.3
4,342,218 - melB b4120 0.58 -0.8
4,342,220 + melB b4120 0.58 +1.0
4,342,228 - melB b4120 0.59 -1.4
4,342,228 - melB b4120 0.59 -1.0
4,342,244 - melB b4120 0.60 -0.3
4,342,261 - melB b4120 0.61 +0.3
4,342,265 - melB b4120 0.61 -0.5
4,342,283 + melB b4120 0.63 -0.2

Or see this region's nucleotide sequence