Experiment: LB Anaerobic with Novobiocin 0.00003125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ymdA and ymdB are separated by 94 nucleotides ymdB and ymdC are separated by 1 nucleotides ymdC and mdoC are separated by 7 nucleotides
b1044: ymdA - hypothetical protein (NCBI), at 1,104,637 to 1,104,948
ymdA
b1045: ymdB - hypothetical protein (NCBI), at 1,105,043 to 1,105,576
ymdB
b1046: ymdC - putative synthase (VIMSS), at 1,105,578 to 1,106,999
ymdC
b1047: mdoC - glucans biosynthesis protein (NCBI), at 1,107,007 to 1,108,164
mdoC
Position (kb)
1105
1106
1107 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1104.597 kb on - strand at 1104.599 kb on + strand at 1104.607 kb on - strand at 1104.708 kb on - strand, within ymdA at 1104.712 kb on + strand, within ymdA at 1104.712 kb on + strand, within ymdA at 1104.712 kb on + strand, within ymdA at 1104.712 kb on + strand, within ymdA at 1104.720 kb on - strand, within ymdA at 1104.720 kb on - strand, within ymdA at 1104.720 kb on - strand, within ymdA at 1104.721 kb on + strand, within ymdA at 1104.764 kb on - strand, within ymdA at 1104.765 kb on + strand, within ymdA at 1104.765 kb on + strand, within ymdA at 1104.770 kb on + strand, within ymdA at 1104.777 kb on + strand, within ymdA at 1104.777 kb on + strand, within ymdA at 1104.777 kb on + strand, within ymdA at 1104.792 kb on - strand, within ymdA at 1104.808 kb on - strand, within ymdA at 1104.812 kb on + strand, within ymdA at 1104.820 kb on - strand, within ymdA at 1104.820 kb on - strand, within ymdA at 1104.886 kb on + strand, within ymdA at 1104.892 kb on - strand, within ymdA at 1104.929 kb on - strand at 1104.929 kb on - strand at 1104.980 kb on - strand at 1104.980 kb on - strand at 1105.072 kb on - strand at 1105.085 kb on - strand at 1105.159 kb on + strand, within ymdB at 1105.159 kb on + strand, within ymdB at 1105.285 kb on - strand, within ymdB at 1105.313 kb on - strand, within ymdB at 1105.313 kb on - strand, within ymdB at 1105.353 kb on + strand, within ymdB at 1105.358 kb on + strand, within ymdB at 1105.414 kb on + strand, within ymdB at 1105.501 kb on + strand, within ymdB at 1105.501 kb on + strand, within ymdB at 1105.501 kb on + strand, within ymdB at 1105.535 kb on + strand at 1105.639 kb on - strand at 1105.729 kb on - strand, within ymdC at 1105.770 kb on + strand, within ymdC at 1105.770 kb on + strand, within ymdC at 1105.770 kb on + strand, within ymdC at 1105.770 kb on + strand, within ymdC at 1105.770 kb on + strand, within ymdC at 1105.770 kb on + strand, within ymdC at 1105.809 kb on + strand, within ymdC at 1105.812 kb on + strand, within ymdC at 1106.017 kb on + strand, within ymdC at 1106.051 kb on - strand, within ymdC at 1106.094 kb on - strand, within ymdC at 1106.112 kb on + strand, within ymdC at 1106.131 kb on - strand, within ymdC at 1106.131 kb on - strand, within ymdC at 1106.303 kb on + strand, within ymdC at 1106.303 kb on + strand, within ymdC at 1106.372 kb on + strand, within ymdC at 1106.403 kb on + strand, within ymdC at 1106.403 kb on + strand, within ymdC at 1106.468 kb on - strand, within ymdC at 1106.468 kb on - strand, within ymdC at 1106.468 kb on - strand, within ymdC at 1106.468 kb on - strand, within ymdC at 1106.474 kb on + strand, within ymdC at 1106.480 kb on - strand, within ymdC at 1106.547 kb on + strand, within ymdC at 1106.554 kb on + strand, within ymdC at 1106.763 kb on + strand, within ymdC at 1106.763 kb on + strand, within ymdC at 1106.764 kb on - strand, within ymdC at 1106.766 kb on + strand, within ymdC at 1106.769 kb on - strand, within ymdC at 1106.769 kb on - strand, within ymdC at 1106.772 kb on + strand, within ymdC at 1106.776 kb on + strand, within ymdC at 1106.833 kb on + strand, within ymdC at 1106.833 kb on + strand, within ymdC at 1106.847 kb on + strand, within ymdC at 1106.847 kb on + strand, within ymdC at 1106.855 kb on - strand, within ymdC at 1106.855 kb on - strand, within ymdC at 1106.864 kb on - strand at 1106.864 kb on - strand at 1106.864 kb on - strand at 1106.864 kb on - strand at 1106.864 kb on - strand at 1106.866 kb on + strand at 1106.866 kb on + strand at 1106.866 kb on + strand at 1106.866 kb on + strand at 1106.874 kb on - strand at 1106.881 kb on + strand at 1106.891 kb on + strand at 1106.903 kb on + strand at 1106.950 kb on + strand at 1106.950 kb on + strand at 1106.964 kb on - strand at 1106.979 kb on + strand at 1106.979 kb on + strand at 1107.010 kb on + strand at 1107.012 kb on - strand at 1107.048 kb on + strand at 1107.048 kb on + strand at 1107.056 kb on - strand at 1107.111 kb on - strand at 1107.116 kb on - strand at 1107.153 kb on + strand, within mdoC at 1107.153 kb on + strand, within mdoC at 1107.161 kb on + strand, within mdoC at 1107.179 kb on - strand, within mdoC at 1107.213 kb on - strand, within mdoC at 1107.213 kb on - strand, within mdoC at 1107.268 kb on - strand, within mdoC at 1107.268 kb on - strand, within mdoC at 1107.420 kb on + strand, within mdoC at 1107.428 kb on - strand, within mdoC at 1107.428 kb on - strand, within mdoC at 1107.439 kb on - strand, within mdoC at 1107.499 kb on + strand, within mdoC at 1107.576 kb on - strand, within mdoC at 1107.576 kb on - strand, within mdoC at 1107.606 kb on - strand, within mdoC at 1107.719 kb on - strand, within mdoC at 1107.721 kb on - strand, within mdoC at 1107.721 kb on - strand, within mdoC at 1107.721 kb on - strand, within mdoC at 1107.721 kb on - strand, within mdoC at 1107.721 kb on - strand, within mdoC at 1107.723 kb on - strand, within mdoC at 1107.728 kb on - strand, within mdoC at 1107.733 kb on - strand, within mdoC at 1107.781 kb on - strand, within mdoC at 1107.792 kb on - strand, within mdoC at 1107.800 kb on - strand, within mdoC at 1107.800 kb on - strand, within mdoC at 1107.800 kb on - strand, within mdoC at 1107.845 kb on - strand, within mdoC at 1107.867 kb on - strand, within mdoC at 1107.867 kb on - strand, within mdoC at 1107.870 kb on - strand, within mdoC at 1107.872 kb on + strand, within mdoC at 1107.878 kb on - strand, within mdoC at 1107.880 kb on - strand, within mdoC at 1107.880 kb on - strand, within mdoC at 1107.880 kb on - strand, within mdoC at 1107.885 kb on + strand, within mdoC at 1107.888 kb on - strand, within mdoC at 1107.929 kb on + strand, within mdoC at 1107.930 kb on - strand, within mdoC
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Novobiocin 0.00003125 mM remove 1,104,597 - +0.5 1,104,599 + -1.5 1,104,607 - -0.5 1,104,708 - ymdA b1044 0.23 -0.1 1,104,712 + ymdA b1044 0.24 -1.5 1,104,712 + ymdA b1044 0.24 +2.1 1,104,712 + ymdA b1044 0.24 +0.5 1,104,712 + ymdA b1044 0.24 -1.1 1,104,720 - ymdA b1044 0.27 -0.2 1,104,720 - ymdA b1044 0.27 -0.1 1,104,720 - ymdA b1044 0.27 +2.1 1,104,721 + ymdA b1044 0.27 -0.5 1,104,764 - ymdA b1044 0.41 +1.7 1,104,765 + ymdA b1044 0.41 +0.5 1,104,765 + ymdA b1044 0.41 -1.1 1,104,770 + ymdA b1044 0.43 +0.5 1,104,777 + ymdA b1044 0.45 -0.5 1,104,777 + ymdA b1044 0.45 -0.3 1,104,777 + ymdA b1044 0.45 -0.2 1,104,792 - ymdA b1044 0.50 -0.4 1,104,808 - ymdA b1044 0.55 +1.5 1,104,812 + ymdA b1044 0.56 -0.1 1,104,820 - ymdA b1044 0.59 +1.3 1,104,820 - ymdA b1044 0.59 -1.0 1,104,886 + ymdA b1044 0.80 -2.3 1,104,892 - ymdA b1044 0.82 -1.0 1,104,929 - -1.5 1,104,929 - +0.2 1,104,980 - -1.5 1,104,980 - -0.1 1,105,072 - +1.0 1,105,085 - +0.1 1,105,159 + ymdB b1045 0.22 -1.5 1,105,159 + ymdB b1045 0.22 -0.2 1,105,285 - ymdB b1045 0.45 -0.5 1,105,313 - ymdB b1045 0.51 -0.1 1,105,313 - ymdB b1045 0.51 -0.4 1,105,353 + ymdB b1045 0.58 -0.4 1,105,358 + ymdB b1045 0.59 +0.1 1,105,414 + ymdB b1045 0.69 +0.5 1,105,501 + ymdB b1045 0.86 -0.6 1,105,501 + ymdB b1045 0.86 -0.5 1,105,501 + ymdB b1045 0.86 +0.3 1,105,535 + +0.0 1,105,639 - -0.5 1,105,729 - ymdC b1046 0.11 +0.6 1,105,770 + ymdC b1046 0.14 +1.1 1,105,770 + ymdC b1046 0.14 -0.1 1,105,770 + ymdC b1046 0.14 -0.5 1,105,770 + ymdC b1046 0.14 -1.4 1,105,770 + ymdC b1046 0.14 +0.8 1,105,770 + ymdC b1046 0.14 +1.5 1,105,809 + ymdC b1046 0.16 +0.9 1,105,812 + ymdC b1046 0.16 -0.2 1,106,017 + ymdC b1046 0.31 -0.7 1,106,051 - ymdC b1046 0.33 -0.0 1,106,094 - ymdC b1046 0.36 +1.5 1,106,112 + ymdC b1046 0.38 -0.7 1,106,131 - ymdC b1046 0.39 -1.5 1,106,131 - ymdC b1046 0.39 -0.5 1,106,303 + ymdC b1046 0.51 +2.1 1,106,303 + ymdC b1046 0.51 -0.5 1,106,372 + ymdC b1046 0.56 -0.1 1,106,403 + ymdC b1046 0.58 -0.0 1,106,403 + ymdC b1046 0.58 +1.0 1,106,468 - ymdC b1046 0.63 +0.2 1,106,468 - ymdC b1046 0.63 +2.1 1,106,468 - ymdC b1046 0.63 +0.5 1,106,468 - ymdC b1046 0.63 +0.2 1,106,474 + ymdC b1046 0.63 -0.1 1,106,480 - ymdC b1046 0.63 -0.0 1,106,547 + ymdC b1046 0.68 -0.4 1,106,554 + ymdC b1046 0.69 +1.1 1,106,763 + ymdC b1046 0.83 +0.7 1,106,763 + ymdC b1046 0.83 +2.5 1,106,764 - ymdC b1046 0.83 -0.3 1,106,766 + ymdC b1046 0.84 +2.1 1,106,769 - ymdC b1046 0.84 +0.2 1,106,769 - ymdC b1046 0.84 +0.8 1,106,772 + ymdC b1046 0.84 +0.3 1,106,776 + ymdC b1046 0.84 -1.1 1,106,833 + ymdC b1046 0.88 -0.7 1,106,833 + ymdC b1046 0.88 +0.5 1,106,847 + ymdC b1046 0.89 +0.3 1,106,847 + ymdC b1046 0.89 -0.3 1,106,855 - ymdC b1046 0.90 +1.1 1,106,855 - ymdC b1046 0.90 +0.5 1,106,864 - -1.5 1,106,864 - +0.5 1,106,864 - -1.1 1,106,864 - +0.7 1,106,864 - -0.8 1,106,866 + -0.7 1,106,866 + -0.9 1,106,866 + -1.4 1,106,866 + -0.2 1,106,874 - -1.1 1,106,881 + +0.5 1,106,891 + +0.8 1,106,903 + +0.1 1,106,950 + +1.2 1,106,950 + +0.8 1,106,964 - +0.5 1,106,979 + -0.2 1,106,979 + +1.5 1,107,010 + -0.0 1,107,012 - +0.2 1,107,048 + -0.5 1,107,048 + -0.4 1,107,056 - +0.3 1,107,111 - -0.2 1,107,116 - +0.5 1,107,153 + mdoC b1047 0.13 -1.5 1,107,153 + mdoC b1047 0.13 -0.5 1,107,161 + mdoC b1047 0.13 -0.8 1,107,179 - mdoC b1047 0.15 +0.3 1,107,213 - mdoC b1047 0.18 -1.6 1,107,213 - mdoC b1047 0.18 +1.5 1,107,268 - mdoC b1047 0.23 -0.5 1,107,268 - mdoC b1047 0.23 +0.5 1,107,420 + mdoC b1047 0.36 +0.5 1,107,428 - mdoC b1047 0.36 +0.5 1,107,428 - mdoC b1047 0.36 +0.5 1,107,439 - mdoC b1047 0.37 +1.5 1,107,499 + mdoC b1047 0.42 +0.5 1,107,576 - mdoC b1047 0.49 +0.5 1,107,576 - mdoC b1047 0.49 +0.5 1,107,606 - mdoC b1047 0.52 +0.5 1,107,719 - mdoC b1047 0.61 +0.5 1,107,721 - mdoC b1047 0.62 +2.5 1,107,721 - mdoC b1047 0.62 -0.5 1,107,721 - mdoC b1047 0.62 +0.5 1,107,721 - mdoC b1047 0.62 +0.7 1,107,721 - mdoC b1047 0.62 -0.8 1,107,723 - mdoC b1047 0.62 -1.1 1,107,728 - mdoC b1047 0.62 -1.5 1,107,733 - mdoC b1047 0.63 -1.1 1,107,781 - mdoC b1047 0.67 -1.0 1,107,792 - mdoC b1047 0.68 +0.8 1,107,800 - mdoC b1047 0.68 +1.1 1,107,800 - mdoC b1047 0.68 -0.3 1,107,800 - mdoC b1047 0.68 -1.1 1,107,845 - mdoC b1047 0.72 -0.3 1,107,867 - mdoC b1047 0.74 -0.8 1,107,867 - mdoC b1047 0.74 -0.6 1,107,870 - mdoC b1047 0.75 +0.1 1,107,872 + mdoC b1047 0.75 +0.5 1,107,878 - mdoC b1047 0.75 -0.2 1,107,880 - mdoC b1047 0.75 +0.2 1,107,880 - mdoC b1047 0.75 +1.2 1,107,880 - mdoC b1047 0.75 +0.7 1,107,885 + mdoC b1047 0.76 +0.5 1,107,888 - mdoC b1047 0.76 -0.5 1,107,929 + mdoC b1047 0.80 +0.5 1,107,930 - mdoC b1047 0.80 +0.5
Or see this region's nucleotide sequence