Experiment: LB 0.1x with 55.5 mM D-Glucose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0438 and PP_0439 overlap by 32 nucleotides PP_0439 and PP_t01 are separated by 152 nucleotides PP_t01 and PP_t02 are separated by 24 nucleotides PP_t02 and PP_t03 are separated by 29 nucleotides PP_t03 and PP_0440 are separated by 78 nucleotides
PP_0438: PP_0438 - Type III pantothenate kinase, at 531,036 to 531,785
_0438
PP_0439: PP_0439 - conserved protein of unknown function, at 531,754 to 532,218
_0439
PP_t01: PP_t01 - tRNA-Tyr, at 532,371 to 532,454
_t01
PP_t02: PP_t02 - tRNA-Gly, at 532,479 to 532,552
_t02
PP_t03: PP_t03 - tRNA-Thr, at 532,582 to 532,657
_t03
PP_0440: PP_0440 - Elongation factor Tu-A, at 532,736 to 533,929
_0440
Position (kb) 532
533 Strain fitness (log2 ratio) -2
-1
0
1
2 at 531.792 kb on + strand at 531.792 kb on + strand at 531.793 kb on - strand at 531.815 kb on + strand, within PP_0439 at 531.836 kb on - strand, within PP_0439 at 531.836 kb on - strand, within PP_0439 at 531.838 kb on + strand, within PP_0439 at 531.839 kb on - strand, within PP_0439 at 531.839 kb on - strand, within PP_0439 at 531.842 kb on + strand, within PP_0439 at 531.842 kb on + strand, within PP_0439 at 531.843 kb on - strand, within PP_0439 at 531.843 kb on - strand, within PP_0439 at 531.898 kb on + strand, within PP_0439 at 531.899 kb on - strand, within PP_0439 at 531.899 kb on - strand, within PP_0439 at 532.172 kb on + strand at 532.658 kb on + strand at 532.658 kb on + strand at 532.658 kb on + strand at 532.658 kb on + strand at 532.658 kb on + strand at 532.658 kb on + strand at 532.660 kb on + strand at 532.710 kb on + strand at 532.742 kb on - strand at 533.263 kb on + strand, within PP_0440 at 533.302 kb on + strand, within PP_0440 at 533.311 kb on + strand, within PP_0440 at 533.312 kb on - strand, within PP_0440
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.1x with 55.5 mM D-Glucose remove 531,792 + -2.3 531,792 + +2.2 531,793 - +0.7 531,815 + PP_0439 0.13 -0.9 531,836 - PP_0439 0.18 -2.3 531,836 - PP_0439 0.18 +0.7 531,838 + PP_0439 0.18 -1.2 531,839 - PP_0439 0.18 +0.7 531,839 - PP_0439 0.18 -2.3 531,842 + PP_0439 0.19 -1.7 531,842 + PP_0439 0.19 -0.3 531,843 - PP_0439 0.19 +0.7 531,843 - PP_0439 0.19 -1.2 531,898 + PP_0439 0.31 -2.5 531,899 - PP_0439 0.31 -0.9 531,899 - PP_0439 0.31 -0.3 532,172 + -1.9 532,658 + -0.9 532,658 + +0.7 532,658 + +0.7 532,658 + -1.9 532,658 + +0.7 532,658 + +0.7 532,660 + -1.5 532,710 + -1.9 532,742 - -0.3 533,263 + PP_0440 0.44 -0.3 533,302 + PP_0440 0.47 -0.7 533,311 + PP_0440 0.48 +0.7 533,312 - PP_0440 0.48 -0.9
Or see this region's nucleotide sequence