Strain Fitness in Pseudomonas putida KT2440 around PP_5141

Experiment: LB 0.1x with 55.5 mM D-Glucose

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPP_5139 and PP_5140 are separated by 83 nucleotidesPP_5140 and PP_5141 are separated by 56 nucleotidesPP_5141 and PP_5142 are separated by 51 nucleotidesPP_5142 and PP_5143 are separated by 16 nucleotides PP_5139: PP_5139 - cadmium translocating P-type ATPase, at 5,863,898 to 5,866,150 _5139 PP_5140: PP_5140 - Transcriptional regulator, MerR family, at 5,866,234 to 5,866,677 _5140 PP_5141: PP_5141 - Thymidylate synthase, at 5,866,734 to 5,867,705 _5141 PP_5142: PP_5142 - Prolipoprotein diacylglyceryl transferase, at 5,867,757 to 5,868,563 _5142 PP_5143: PP_5143 - conserved membrane protein of unknown function, at 5,868,580 to 5,869,362 _5143 Position (kb) 5866 5867 5868Strain fitness (log2 ratio) -3 -2 -1 0 1at 5866.203 kb on + strandat 5866.288 kb on + strand, within PP_5140at 5866.288 kb on + strand, within PP_5140at 5866.291 kb on + strand, within PP_5140at 5866.292 kb on - strand, within PP_5140at 5866.292 kb on - strand, within PP_5140at 5866.339 kb on + strandat 5866.340 kb on - strand, within PP_5140at 5866.349 kb on - strand, within PP_5140at 5866.724 kb on + strandat 5866.725 kb on - strandat 5866.725 kb on - strandat 5866.732 kb on + strandat 5866.732 kb on + strandat 5866.732 kb on + strandat 5866.732 kb on + strandat 5867.775 kb on - strandat 5867.787 kb on - strandat 5867.787 kb on - strandat 5867.789 kb on - strandat 5867.789 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB 0.1x with 55.5 mM D-Glucose
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5,866,203 + -0.6
5,866,288 + PP_5140 0.12 +0.6
5,866,288 + PP_5140 0.12 -1.9
5,866,291 + PP_5140 0.13 -0.9
5,866,292 - PP_5140 0.13 -2.5
5,866,292 - PP_5140 0.13 -3.3
5,866,339 + -0.4
5,866,340 - PP_5140 0.24 -1.4
5,866,349 - PP_5140 0.26 -1.4
5,866,724 + -1.5
5,866,725 - -1.8
5,866,725 - -0.3
5,866,732 + +0.7
5,866,732 + -0.1
5,866,732 + -1.8
5,866,732 + -1.1
5,867,775 - -0.3
5,867,787 - -1.8
5,867,787 - -1.4
5,867,789 - -0.2
5,867,789 - +0.6

Or see this region's nucleotide sequence