Strain Fitness in Escherichia coli BW25113 around b4244

Experiment: LB Anaerobic with Cefoxitin 0.0000015625 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntmgtA and yjgF are separated by 205 nucleotidesyjgF and pyrI are separated by 72 nucleotidespyrI and pyrB are separated by 12 nucleotidespyrB and pyrL are separated by 3 nucleotides b4242: mgtA - magnesium transporter (NCBI), at 4,465,648 to 4,468,344 mgtA b4243: yjgF - orf, hypothetical protein (VIMSS), at 4,468,550 to 4,468,936 yjgF b4244: pyrI - aspartate carbamoyltransferase regulatory subunit (NCBI), at 4,469,009 to 4,469,470 pyrI b4245: pyrB - aspartate carbamoyltransferase catalytic subunit (NCBI), at 4,469,483 to 4,470,418 pyrB b4246: pyrL - pyrBI operon leader peptide (NCBI), at 4,470,422 to 4,470,556 pyrL Position (kb) 4469 4470Strain fitness (log2 ratio) -2 -1 0 1 2 3at 4468.013 kb on + strand, within mgtAat 4468.058 kb on - strand, within mgtAat 4468.058 kb on - strand, within mgtAat 4468.072 kb on - strand, within mgtAat 4468.097 kb on + strandat 4468.112 kb on + strandat 4468.112 kb on + strandat 4468.135 kb on - strandat 4468.137 kb on + strandat 4468.147 kb on - strandat 4468.212 kb on + strandat 4468.258 kb on - strandat 4468.267 kb on + strandat 4468.267 kb on + strandat 4468.284 kb on + strandat 4468.284 kb on + strandat 4468.285 kb on - strandat 4468.292 kb on - strandat 4468.292 kb on - strandat 4468.292 kb on - strandat 4468.346 kb on + strandat 4468.346 kb on + strandat 4468.346 kb on + strandat 4468.466 kb on + strandat 4468.466 kb on + strandat 4468.466 kb on + strandat 4468.466 kb on + strandat 4468.470 kb on + strandat 4468.519 kb on - strandat 4468.521 kb on + strandat 4468.521 kb on + strandat 4468.598 kb on - strand, within yjgFat 4468.615 kb on + strand, within yjgFat 4468.615 kb on + strand, within yjgFat 4468.622 kb on - strand, within yjgFat 4468.648 kb on + strand, within yjgFat 4468.658 kb on - strandat 4468.673 kb on + strand, within yjgFat 4468.743 kb on - strand, within yjgFat 4468.743 kb on - strand, within yjgFat 4468.743 kb on - strand, within yjgFat 4468.744 kb on - strand, within yjgFat 4468.744 kb on - strand, within yjgFat 4468.749 kb on + strand, within yjgFat 4468.757 kb on - strand, within yjgFat 4468.757 kb on - strand, within yjgFat 4468.860 kb on + strand, within yjgFat 4468.890 kb on - strand, within yjgFat 4468.890 kb on - strand, within yjgFat 4468.898 kb on - strandat 4468.899 kb on - strandat 4469.025 kb on + strandat 4469.025 kb on + strandat 4469.059 kb on - strand, within pyrIat 4469.059 kb on - strand, within pyrIat 4469.121 kb on - strand, within pyrIat 4469.140 kb on - strand, within pyrIat 4469.155 kb on - strand, within pyrIat 4469.157 kb on + strand, within pyrIat 4469.165 kb on - strand, within pyrIat 4469.179 kb on - strand, within pyrIat 4469.179 kb on - strand, within pyrIat 4469.186 kb on + strand, within pyrIat 4469.199 kb on + strand, within pyrIat 4469.204 kb on + strand, within pyrIat 4469.208 kb on - strand, within pyrIat 4469.218 kb on + strand, within pyrIat 4469.245 kb on + strand, within pyrIat 4469.245 kb on + strand, within pyrIat 4469.348 kb on - strand, within pyrIat 4469.366 kb on - strand, within pyrIat 4469.379 kb on - strand, within pyrIat 4469.379 kb on - strand, within pyrIat 4469.414 kb on + strand, within pyrIat 4469.422 kb on - strand, within pyrIat 4469.442 kb on - strandat 4469.501 kb on + strandat 4469.560 kb on - strandat 4469.562 kb on + strandat 4469.579 kb on + strand, within pyrBat 4469.579 kb on + strand, within pyrBat 4469.648 kb on + strand, within pyrBat 4469.715 kb on - strand, within pyrBat 4469.744 kb on - strand, within pyrBat 4469.746 kb on - strand, within pyrBat 4469.751 kb on + strand, within pyrBat 4469.777 kb on + strand, within pyrBat 4469.780 kb on + strand, within pyrBat 4469.780 kb on + strand, within pyrBat 4469.787 kb on + strand, within pyrBat 4469.793 kb on - strand, within pyrBat 4469.793 kb on - strand, within pyrBat 4469.817 kb on + strand, within pyrBat 4469.937 kb on - strand, within pyrBat 4469.945 kb on + strand, within pyrBat 4469.952 kb on + strand, within pyrBat 4469.966 kb on + strand, within pyrBat 4470.043 kb on + strand, within pyrBat 4470.104 kb on + strand, within pyrBat 4470.104 kb on + strand, within pyrBat 4470.106 kb on + strand, within pyrBat 4470.200 kb on + strand, within pyrBat 4470.224 kb on + strand, within pyrBat 4470.257 kb on + strand, within pyrBat 4470.359 kb on - strandat 4470.427 kb on - strandat 4470.427 kb on - strandat 4470.466 kb on + strand, within pyrLat 4470.466 kb on + strand, within pyrL

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Anaerobic with Cefoxitin 0.0000015625 mM
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4,468,013 + mgtA b4242 0.88 +0.8
4,468,058 - mgtA b4242 0.89 +0.8
4,468,058 - mgtA b4242 0.89 +1.4
4,468,072 - mgtA b4242 0.90 +0.0
4,468,097 + +0.3
4,468,112 + -1.2
4,468,112 + +0.8
4,468,135 - +0.3
4,468,137 + +0.3
4,468,147 - -1.0
4,468,212 + -0.2
4,468,258 - +0.3
4,468,267 + +2.4
4,468,267 + +0.8
4,468,284 + +0.4
4,468,284 + +3.2
4,468,285 - -0.4
4,468,292 - -1.2
4,468,292 - -0.2
4,468,292 - +2.4
4,468,346 + -0.2
4,468,346 + -0.2
4,468,346 + +0.8
4,468,466 + -0.2
4,468,466 + +0.6
4,468,466 + -0.8
4,468,466 + +1.4
4,468,470 + +0.4
4,468,519 - -1.2
4,468,521 + -1.7
4,468,521 + -0.9
4,468,598 - yjgF b4243 0.12 +0.8
4,468,615 + yjgF b4243 0.17 -0.9
4,468,615 + yjgF b4243 0.17 -0.4
4,468,622 - yjgF b4243 0.19 -0.4
4,468,648 + yjgF b4243 0.25 -0.4
4,468,658 - -0.2
4,468,673 + yjgF b4243 0.32 +0.8
4,468,743 - yjgF b4243 0.50 +1.3
4,468,743 - yjgF b4243 0.50 +0.8
4,468,743 - yjgF b4243 0.50 +0.0
4,468,744 - yjgF b4243 0.50 -0.3
4,468,744 - yjgF b4243 0.50 -0.0
4,468,749 + yjgF b4243 0.51 +0.0
4,468,757 - yjgF b4243 0.53 +2.1
4,468,757 - yjgF b4243 0.53 -0.2
4,468,860 + yjgF b4243 0.80 +0.5
4,468,890 - yjgF b4243 0.88 -0.5
4,468,890 - yjgF b4243 0.88 +0.1
4,468,898 - -0.6
4,468,899 - +0.6
4,469,025 + -0.7
4,469,025 + +0.5
4,469,059 - pyrI b4244 0.11 +1.1
4,469,059 - pyrI b4244 0.11 +0.3
4,469,121 - pyrI b4244 0.24 -0.4
4,469,140 - pyrI b4244 0.28 -0.2
4,469,155 - pyrI b4244 0.32 +0.1
4,469,157 + pyrI b4244 0.32 -1.3
4,469,165 - pyrI b4244 0.34 +0.6
4,469,179 - pyrI b4244 0.37 -2.2
4,469,179 - pyrI b4244 0.37 -0.6
4,469,186 + pyrI b4244 0.38 -0.1
4,469,199 + pyrI b4244 0.41 +0.6
4,469,204 + pyrI b4244 0.42 +0.1
4,469,208 - pyrI b4244 0.43 +0.2
4,469,218 + pyrI b4244 0.45 -0.5
4,469,245 + pyrI b4244 0.51 +0.4
4,469,245 + pyrI b4244 0.51 -0.9
4,469,348 - pyrI b4244 0.73 -0.0
4,469,366 - pyrI b4244 0.77 -0.8
4,469,379 - pyrI b4244 0.80 -1.5
4,469,379 - pyrI b4244 0.80 -1.2
4,469,414 + pyrI b4244 0.88 -0.6
4,469,422 - pyrI b4244 0.89 +0.1
4,469,442 - +0.1
4,469,501 + -0.2
4,469,560 - +0.6
4,469,562 + +1.8
4,469,579 + pyrB b4245 0.10 +2.6
4,469,579 + pyrB b4245 0.10 -0.9
4,469,648 + pyrB b4245 0.18 -0.5
4,469,715 - pyrB b4245 0.25 +0.1
4,469,744 - pyrB b4245 0.28 -0.8
4,469,746 - pyrB b4245 0.28 +0.1
4,469,751 + pyrB b4245 0.29 -0.3
4,469,777 + pyrB b4245 0.31 +0.6
4,469,780 + pyrB b4245 0.32 -0.3
4,469,780 + pyrB b4245 0.32 +0.8
4,469,787 + pyrB b4245 0.32 -0.2
4,469,793 - pyrB b4245 0.33 -0.6
4,469,793 - pyrB b4245 0.33 -0.5
4,469,817 + pyrB b4245 0.36 +0.2
4,469,937 - pyrB b4245 0.49 -0.1
4,469,945 + pyrB b4245 0.49 -0.5
4,469,952 + pyrB b4245 0.50 -0.7
4,469,966 + pyrB b4245 0.52 +0.3
4,470,043 + pyrB b4245 0.60 +1.5
4,470,104 + pyrB b4245 0.66 -0.1
4,470,104 + pyrB b4245 0.66 -0.2
4,470,106 + pyrB b4245 0.67 -0.3
4,470,200 + pyrB b4245 0.77 -0.4
4,470,224 + pyrB b4245 0.79 -0.2
4,470,257 + pyrB b4245 0.83 -0.8
4,470,359 - +0.4
4,470,427 - +1.8
4,470,427 - -0.4
4,470,466 + pyrL b4246 0.33 +0.0
4,470,466 + pyrL b4246 0.33 +0.2

Or see this region's nucleotide sequence