Strain Fitness in Escherichia coli BW25113 around b0091

Experiment: LB Anaerobic with Cefoxitin 0.0000015625 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntmurG and murC are separated by 53 nucleotidesmurC and ddlB overlap by 8 nucleotidesddlB and ftsQ are separated by 1 nucleotides b0090: murG - N-acetylglucosaminyl transferase (NCBI), at 99,644 to 100,711 murG b0091: murC - UDP-N-acetylmuramate--L-alanine ligase (NCBI), at 100,765 to 102,240 murC b0092: ddlB - D-alanylalanine synthetase (NCBI), at 102,233 to 103,153 ddlB b0093: ftsQ - membrane anchored protein involved in growth of wall at septum (NCBI), at 103,155 to 103,985 ftsQ Position (kb) 100 101 102 103Strain fitness (log2 ratio) -2 -1 0 1at 100.705 kb on + strandat 100.709 kb on + strandat 100.709 kb on + strandat 102.254 kb on + strandat 102.265 kb on + strandat 102.265 kb on + strandat 102.295 kb on + strandat 102.446 kb on + strand, within ddlBat 102.492 kb on + strand, within ddlBat 102.492 kb on + strand, within ddlBat 102.496 kb on + strand, within ddlBat 102.498 kb on + strand, within ddlBat 102.573 kb on + strand, within ddlBat 102.573 kb on + strand, within ddlBat 102.573 kb on + strand, within ddlBat 102.793 kb on + strand, within ddlBat 102.829 kb on + strand, within ddlBat 102.984 kb on + strand, within ddlBat 103.002 kb on + strand, within ddlBat 103.017 kb on + strand, within ddlBat 103.068 kb on + strandat 103.138 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Anaerobic with Cefoxitin 0.0000015625 mM
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100,705 + +1.0
100,709 + -1.6
100,709 + -1.9
102,254 + -0.3
102,265 + -0.5
102,265 + -1.1
102,295 + -1.4
102,446 + ddlB b0092 0.23 -0.9
102,492 + ddlB b0092 0.28 -0.7
102,492 + ddlB b0092 0.28 +0.7
102,496 + ddlB b0092 0.29 +0.5
102,498 + ddlB b0092 0.29 -0.9
102,573 + ddlB b0092 0.37 -0.2
102,573 + ddlB b0092 0.37 -2.4
102,573 + ddlB b0092 0.37 -1.3
102,793 + ddlB b0092 0.61 +0.3
102,829 + ddlB b0092 0.65 -0.3
102,984 + ddlB b0092 0.82 -0.7
103,002 + ddlB b0092 0.83 +0.7
103,017 + ddlB b0092 0.85 -0.3
103,068 + -0.9
103,138 + -0.9

Or see this region's nucleotide sequence