Strain Fitness in Escherichia coli BW25113 around b0086

Experiment: LB Anaerobic with Cefoxitin 0.0000015625 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntmurE and murF overlap by 4 nucleotidesmurF and mraY overlap by 7 nucleotides b0085: murE - UDP-N-acetylmuramoylalanyl-D-glutamate--2, 6-diaminopimelate ligase (NCBI), at 93,166 to 94,653 murE b0086: murF - UDP-N-acetylmuramoyl-tripeptide:D-alanyl-D- alanine ligase (NCBI), at 94,650 to 96,008 murF b0087: mraY - phospho-N-acetylmuramoyl-pentapeptide-transferase (NCBI), at 96,002 to 97,084 mraY Position (kb) 94 95 96 97Strain fitness (log2 ratio) -1 0 1at 94.617 kb on + strandat 94.634 kb on + strandat 95.980 kb on + strandat 95.980 kb on + strandat 95.983 kb on + strandat 95.983 kb on + strandat 95.985 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Anaerobic with Cefoxitin 0.0000015625 mM
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94,617 + -1.1
94,634 + +0.2
95,980 + -0.8
95,980 + -1.3
95,983 + -1.3
95,983 + -0.1
95,985 + +0.4

Or see this region's nucleotide sequence