Experiment: LB 0.1x with 55.5 mM D-Glucose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0612 and PP_0613 are separated by 76 nucleotides PP_0613 and PP_0614 are separated by 1 nucleotides
PP_0612: PP_0612 - FAD-dependent glycine/D-amino acid oxidase, at 717,333 to 718,430
_0612
PP_0613: PP_0613 - Amidase family protein, at 718,507 to 719,916
_0613
PP_0614: PP_0614 - putative N-carbamoyl-beta-alanine amidohydrolase/allantoine amidohydrolase 1, at 719,918 to 721,195
_0614
Position (kb)
718
719
720 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 717.543 kb on + strand, within PP_0612 at 717.612 kb on + strand, within PP_0612 at 717.702 kb on + strand, within PP_0612 at 717.702 kb on + strand, within PP_0612 at 717.747 kb on + strand, within PP_0612 at 717.747 kb on + strand, within PP_0612 at 717.747 kb on + strand, within PP_0612 at 717.747 kb on + strand, within PP_0612 at 717.747 kb on + strand, within PP_0612 at 717.747 kb on + strand, within PP_0612 at 717.748 kb on - strand, within PP_0612 at 717.748 kb on - strand, within PP_0612 at 717.748 kb on - strand, within PP_0612 at 717.748 kb on - strand, within PP_0612 at 717.748 kb on - strand, within PP_0612 at 717.748 kb on - strand, within PP_0612 at 717.748 kb on - strand, within PP_0612 at 717.889 kb on + strand, within PP_0612 at 717.889 kb on + strand, within PP_0612 at 717.889 kb on + strand, within PP_0612 at 717.890 kb on - strand, within PP_0612 at 717.965 kb on + strand, within PP_0612 at 718.263 kb on + strand, within PP_0612 at 718.263 kb on + strand, within PP_0612 at 718.263 kb on + strand, within PP_0612 at 718.263 kb on + strand, within PP_0612 at 718.263 kb on + strand, within PP_0612 at 718.263 kb on + strand, within PP_0612 at 718.264 kb on - strand, within PP_0612 at 718.314 kb on + strand, within PP_0612 at 718.404 kb on + strand at 718.404 kb on + strand at 718.458 kb on + strand at 718.459 kb on - strand at 718.459 kb on - strand at 718.459 kb on - strand at 718.515 kb on + strand at 718.516 kb on - strand at 718.556 kb on + strand at 718.557 kb on - strand at 718.557 kb on - strand at 718.688 kb on + strand, within PP_0613 at 718.796 kb on + strand, within PP_0613 at 718.859 kb on + strand, within PP_0613 at 718.859 kb on + strand, within PP_0613 at 718.859 kb on + strand, within PP_0613 at 718.860 kb on - strand, within PP_0613 at 719.026 kb on + strand, within PP_0613 at 719.026 kb on + strand, within PP_0613 at 719.103 kb on - strand, within PP_0613 at 719.131 kb on + strand, within PP_0613 at 719.131 kb on + strand, within PP_0613 at 719.132 kb on - strand, within PP_0613 at 719.132 kb on - strand, within PP_0613 at 719.132 kb on - strand, within PP_0613 at 719.132 kb on - strand, within PP_0613 at 719.132 kb on - strand, within PP_0613 at 719.312 kb on + strand, within PP_0613 at 719.313 kb on - strand, within PP_0613 at 719.313 kb on - strand, within PP_0613 at 719.619 kb on + strand, within PP_0613 at 719.623 kb on - strand, within PP_0613 at 719.861 kb on + strand at 719.861 kb on + strand at 719.862 kb on - strand at 719.862 kb on - strand at 720.108 kb on + strand, within PP_0614 at 720.174 kb on + strand, within PP_0614 at 720.317 kb on + strand, within PP_0614 at 720.318 kb on - strand, within PP_0614 at 720.318 kb on - strand, within PP_0614 at 720.590 kb on - strand, within PP_0614 at 720.604 kb on - strand, within PP_0614 at 720.646 kb on - strand, within PP_0614 at 720.788 kb on + strand, within PP_0614 at 720.788 kb on + strand, within PP_0614
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.1x with 55.5 mM D-Glucose remove 717,543 + PP_0612 0.19 -0.3 717,612 + PP_0612 0.25 +0.4 717,702 + PP_0612 0.34 +0.9 717,702 + PP_0612 0.34 -1.9 717,747 + PP_0612 0.38 +1.5 717,747 + PP_0612 0.38 -0.3 717,747 + PP_0612 0.38 +1.2 717,747 + PP_0612 0.38 +1.9 717,747 + PP_0612 0.38 -0.6 717,747 + PP_0612 0.38 -1.1 717,748 - PP_0612 0.38 +1.3 717,748 - PP_0612 0.38 +0.1 717,748 - PP_0612 0.38 -1.5 717,748 - PP_0612 0.38 -0.6 717,748 - PP_0612 0.38 +1.0 717,748 - PP_0612 0.38 -0.8 717,748 - PP_0612 0.38 +0.2 717,889 + PP_0612 0.51 -0.1 717,889 + PP_0612 0.51 -0.7 717,889 + PP_0612 0.51 -0.4 717,890 - PP_0612 0.51 +0.1 717,965 + PP_0612 0.58 -1.6 718,263 + PP_0612 0.85 +0.3 718,263 + PP_0612 0.85 +1.3 718,263 + PP_0612 0.85 +0.2 718,263 + PP_0612 0.85 +0.0 718,263 + PP_0612 0.85 -0.4 718,263 + PP_0612 0.85 -0.2 718,264 - PP_0612 0.85 -0.5 718,314 + PP_0612 0.89 +0.1 718,404 + -0.6 718,404 + -0.4 718,458 + +0.2 718,459 - -0.8 718,459 - -1.3 718,459 - -0.6 718,515 + +0.2 718,516 - +0.4 718,556 + -0.9 718,557 - -0.1 718,557 - +0.5 718,688 + PP_0613 0.13 -2.0 718,796 + PP_0613 0.20 -0.1 718,859 + PP_0613 0.25 +0.4 718,859 + PP_0613 0.25 +0.5 718,859 + PP_0613 0.25 +0.2 718,860 - PP_0613 0.25 -1.7 719,026 + PP_0613 0.37 -0.3 719,026 + PP_0613 0.37 -1.4 719,103 - PP_0613 0.42 -0.6 719,131 + PP_0613 0.44 -1.3 719,131 + PP_0613 0.44 -1.0 719,132 - PP_0613 0.44 +1.1 719,132 - PP_0613 0.44 -1.8 719,132 - PP_0613 0.44 -0.0 719,132 - PP_0613 0.44 -0.1 719,132 - PP_0613 0.44 -1.0 719,312 + PP_0613 0.57 -0.2 719,313 - PP_0613 0.57 -0.5 719,313 - PP_0613 0.57 -0.0 719,619 + PP_0613 0.79 +0.8 719,623 - PP_0613 0.79 -0.8 719,861 + +0.3 719,861 + -0.2 719,862 - +0.4 719,862 - -0.2 720,108 + PP_0614 0.15 -0.3 720,174 + PP_0614 0.20 -0.8 720,317 + PP_0614 0.31 -1.5 720,318 - PP_0614 0.31 +0.3 720,318 - PP_0614 0.31 -0.9 720,590 - PP_0614 0.53 -0.2 720,604 - PP_0614 0.54 -0.2 720,646 - PP_0614 0.57 +1.5 720,788 + PP_0614 0.68 +0.5 720,788 + PP_0614 0.68 -0.2
Or see this region's nucleotide sequence