Strain Fitness in Escherichia coli BW25113 around b4169

Experiment: LB Anaerobic with Cefoxitin 0.000003125 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyjeF and yjeE overlap by 29 nucleotidesyjeE and amiB are separated by 18 nucleotidesamiB and mutL are separated by 9 nucleotides b4167: yjeF - predicted carbohydrate kinase (NCBI), at 4,392,089 to 4,393,636 yjeF b4168: yjeE - ATPase with strong ADP affinity (NCBI), at 4,393,608 to 4,394,069 yjeE b4169: amiB - N-acetylmuramoyl-l-alanine amidase II (NCBI), at 4,394,088 to 4,395,425 amiB b4170: mutL - DNA mismatch repair protein (NCBI), at 4,395,435 to 4,397,282 mutL Position (kb) 4394 4395 4396Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 4393.194 kb on + strand, within yjeFat 4393.306 kb on - strand, within yjeFat 4393.396 kb on - strand, within yjeFat 4393.446 kb on + strand, within yjeFat 4393.446 kb on + strand, within yjeFat 4393.468 kb on - strand, within yjeFat 4393.474 kb on + strand, within yjeFat 4393.476 kb on + strand, within yjeFat 4393.476 kb on + strand, within yjeFat 4393.479 kb on - strand, within yjeFat 4393.490 kb on - strandat 4393.497 kb on + strandat 4393.510 kb on + strandat 4393.543 kb on + strandat 4393.566 kb on - strandat 4393.584 kb on + strandat 4394.078 kb on - strandat 4394.123 kb on + strandat 4394.142 kb on + strandat 4394.143 kb on - strandat 4394.232 kb on - strand, within amiBat 4394.232 kb on - strand, within amiBat 4394.244 kb on + strand, within amiBat 4394.257 kb on + strand, within amiBat 4394.257 kb on + strand, within amiBat 4394.294 kb on - strand, within amiBat 4394.294 kb on - strand, within amiBat 4394.345 kb on - strand, within amiBat 4394.352 kb on - strand, within amiBat 4394.352 kb on - strand, within amiBat 4394.353 kb on - strand, within amiBat 4394.389 kb on + strand, within amiBat 4394.424 kb on + strand, within amiBat 4394.424 kb on + strand, within amiBat 4394.454 kb on + strand, within amiBat 4394.553 kb on + strand, within amiBat 4394.553 kb on + strand, within amiBat 4394.603 kb on - strand, within amiBat 4394.603 kb on - strand, within amiBat 4394.606 kb on - strand, within amiBat 4394.606 kb on - strand, within amiBat 4394.642 kb on + strand, within amiBat 4394.650 kb on - strand, within amiBat 4394.755 kb on + strand, within amiBat 4394.798 kb on - strand, within amiBat 4394.832 kb on - strand, within amiBat 4394.832 kb on - strand, within amiBat 4394.850 kb on + strand, within amiBat 4394.856 kb on - strand, within amiBat 4394.856 kb on - strand, within amiBat 4394.912 kb on + strand, within amiBat 4394.917 kb on - strand, within amiBat 4394.939 kb on - strand, within amiBat 4394.939 kb on - strand, within amiBat 4394.942 kb on + strand, within amiBat 4394.943 kb on + strand, within amiBat 4394.944 kb on + strand, within amiBat 4394.944 kb on + strand, within amiBat 4394.951 kb on - strand, within amiBat 4394.951 kb on - strand, within amiBat 4395.041 kb on + strand, within amiBat 4395.080 kb on - strand, within amiBat 4395.080 kb on - strand, within amiBat 4395.112 kb on + strand, within amiBat 4395.112 kb on + strand, within amiBat 4395.116 kb on - strand, within amiBat 4395.214 kb on + strand, within amiBat 4395.217 kb on + strand, within amiBat 4395.246 kb on + strand, within amiBat 4395.286 kb on + strand, within amiBat 4395.307 kb on - strandat 4395.307 kb on - strandat 4395.348 kb on + strandat 4395.348 kb on + strandat 4395.414 kb on - strandat 4395.454 kb on + strandat 4395.596 kb on - strandat 4395.655 kb on + strand, within mutLat 4395.679 kb on + strand, within mutLat 4395.679 kb on + strand, within mutLat 4395.775 kb on + strand, within mutLat 4395.808 kb on - strand, within mutLat 4395.808 kb on - strand, within mutLat 4395.808 kb on - strand, within mutLat 4395.853 kb on + strand, within mutLat 4395.854 kb on + strand, within mutLat 4395.854 kb on + strand, within mutLat 4395.861 kb on - strand, within mutLat 4395.865 kb on + strand, within mutLat 4395.865 kb on + strand, within mutLat 4395.872 kb on - strand, within mutLat 4395.872 kb on - strand, within mutLat 4395.873 kb on - strand, within mutLat 4395.873 kb on - strand, within mutLat 4395.920 kb on + strand, within mutLat 4395.920 kb on + strand, within mutLat 4395.986 kb on - strand, within mutLat 4395.986 kb on - strand, within mutLat 4396.002 kb on + strand, within mutLat 4396.002 kb on + strand, within mutLat 4396.079 kb on + strand, within mutLat 4396.148 kb on - strand, within mutLat 4396.248 kb on + strand, within mutLat 4396.271 kb on + strand, within mutLat 4396.271 kb on + strand, within mutLat 4396.326 kb on - strand, within mutLat 4396.329 kb on - strand, within mutLat 4396.407 kb on + strand, within mutLat 4396.415 kb on + strand, within mutLat 4396.415 kb on - strand, within mutL

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Anaerobic with Cefoxitin 0.000003125 mM
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4,393,194 + yjeF b4167 0.71 -0.6
4,393,306 - yjeF b4167 0.79 +0.4
4,393,396 - yjeF b4167 0.84 -0.5
4,393,446 + yjeF b4167 0.88 -0.8
4,393,446 + yjeF b4167 0.88 +0.2
4,393,468 - yjeF b4167 0.89 -0.5
4,393,474 + yjeF b4167 0.89 -0.9
4,393,476 + yjeF b4167 0.90 -0.6
4,393,476 + yjeF b4167 0.90 +1.8
4,393,479 - yjeF b4167 0.90 -0.1
4,393,490 - -1.6
4,393,497 + -1.7
4,393,510 + +0.3
4,393,543 + -0.3
4,393,566 - -0.1
4,393,584 + -1.3
4,394,078 - +0.3
4,394,123 + +0.9
4,394,142 + -0.4
4,394,143 - -0.9
4,394,232 - amiB b4169 0.11 -0.4
4,394,232 - amiB b4169 0.11 -0.8
4,394,244 + amiB b4169 0.12 +0.1
4,394,257 + amiB b4169 0.13 -0.1
4,394,257 + amiB b4169 0.13 -0.4
4,394,294 - amiB b4169 0.15 +0.3
4,394,294 - amiB b4169 0.15 -0.2
4,394,345 - amiB b4169 0.19 +0.4
4,394,352 - amiB b4169 0.20 -0.3
4,394,352 - amiB b4169 0.20 -0.7
4,394,353 - amiB b4169 0.20 +1.2
4,394,389 + amiB b4169 0.22 -0.3
4,394,424 + amiB b4169 0.25 +0.5
4,394,424 + amiB b4169 0.25 -0.2
4,394,454 + amiB b4169 0.27 -0.7
4,394,553 + amiB b4169 0.35 +0.2
4,394,553 + amiB b4169 0.35 -0.4
4,394,603 - amiB b4169 0.38 +0.1
4,394,603 - amiB b4169 0.38 -0.2
4,394,606 - amiB b4169 0.39 +0.3
4,394,606 - amiB b4169 0.39 -0.3
4,394,642 + amiB b4169 0.41 -0.2
4,394,650 - amiB b4169 0.42 -0.0
4,394,755 + amiB b4169 0.50 +1.1
4,394,798 - amiB b4169 0.53 +0.1
4,394,832 - amiB b4169 0.56 +0.2
4,394,832 - amiB b4169 0.56 -1.6
4,394,850 + amiB b4169 0.57 -0.6
4,394,856 - amiB b4169 0.57 -0.4
4,394,856 - amiB b4169 0.57 +0.1
4,394,912 + amiB b4169 0.62 -0.0
4,394,917 - amiB b4169 0.62 -0.0
4,394,939 - amiB b4169 0.64 -1.1
4,394,939 - amiB b4169 0.64 -0.4
4,394,942 + amiB b4169 0.64 -0.2
4,394,943 + amiB b4169 0.64 -3.0
4,394,944 + amiB b4169 0.64 -0.7
4,394,944 + amiB b4169 0.64 -1.3
4,394,951 - amiB b4169 0.64 -0.4
4,394,951 - amiB b4169 0.64 +0.7
4,395,041 + amiB b4169 0.71 -0.4
4,395,080 - amiB b4169 0.74 -0.0
4,395,080 - amiB b4169 0.74 +0.1
4,395,112 + amiB b4169 0.77 +0.5
4,395,112 + amiB b4169 0.77 -0.4
4,395,116 - amiB b4169 0.77 +0.1
4,395,214 + amiB b4169 0.84 -0.2
4,395,217 + amiB b4169 0.84 -0.5
4,395,246 + amiB b4169 0.87 -0.1
4,395,286 + amiB b4169 0.90 -0.2
4,395,307 - -0.2
4,395,307 - +1.0
4,395,348 + -1.6
4,395,348 + +0.4
4,395,414 - -0.2
4,395,454 + +0.3
4,395,596 - -1.0
4,395,655 + mutL b4170 0.12 +0.4
4,395,679 + mutL b4170 0.13 -0.8
4,395,679 + mutL b4170 0.13 +1.8
4,395,775 + mutL b4170 0.18 -0.6
4,395,808 - mutL b4170 0.20 -0.3
4,395,808 - mutL b4170 0.20 -1.3
4,395,808 - mutL b4170 0.20 +1.6
4,395,853 + mutL b4170 0.23 -0.1
4,395,854 + mutL b4170 0.23 -0.0
4,395,854 + mutL b4170 0.23 +0.5
4,395,861 - mutL b4170 0.23 +2.6
4,395,865 + mutL b4170 0.23 +1.4
4,395,865 + mutL b4170 0.23 +0.1
4,395,872 - mutL b4170 0.24 -0.5
4,395,872 - mutL b4170 0.24 +0.6
4,395,873 - mutL b4170 0.24 +0.2
4,395,873 - mutL b4170 0.24 -0.3
4,395,920 + mutL b4170 0.26 +0.6
4,395,920 + mutL b4170 0.26 -0.0
4,395,986 - mutL b4170 0.30 -1.4
4,395,986 - mutL b4170 0.30 +0.4
4,396,002 + mutL b4170 0.31 +0.2
4,396,002 + mutL b4170 0.31 -0.3
4,396,079 + mutL b4170 0.35 -0.2
4,396,148 - mutL b4170 0.39 +0.7
4,396,248 + mutL b4170 0.44 -0.5
4,396,271 + mutL b4170 0.45 +0.5
4,396,271 + mutL b4170 0.45 -0.4
4,396,326 - mutL b4170 0.48 +0.5
4,396,329 - mutL b4170 0.48 -0.2
4,396,407 + mutL b4170 0.53 -0.6
4,396,415 + mutL b4170 0.53 +0.4
4,396,415 - mutL b4170 0.53 -0.1

Or see this region's nucleotide sequence