Strain Fitness in Escherichia coli BW25113 around b2095

Experiment: LB Anaerobic with Cefoxitin 0.000003125 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntgatC and gatB are separated by 3 nucleotidesgatB and gatA are separated by 30 nucleotidesgatA and gatZ are separated by 9 nucleotidesgatZ and gatY are separated by 28 nucleotides b2092: gatC - galactitol-specific enzyme IIC component of PTS (NCBI), at 2,170,945 to 2,172,300 gatC b2093: gatB - galactitol-specific enzyme IIB component of PTS (NCBI), at 2,172,304 to 2,172,588 gatB b2094: gatA - galactitol-specific enzyme IIA component of PTS (NCBI), at 2,172,619 to 2,173,071 gatA b2095: gatZ - D-tagatose 1,6-bisphosphate aldolase 2, subunit (NCBI), at 2,173,081 to 2,174,343 gatZ b2096: gatY - tagatose-bisphosphate aldolase 1 (VIMSS), at 2,174,372 to 2,175,226 gatY Position (kb) 2173 2174 2175Strain fitness (log2 ratio) -1 0 1 2 3 4at 2172.130 kb on + strand, within gatCat 2172.137 kb on - strand, within gatCat 2172.247 kb on + strandat 2172.255 kb on + strandat 2172.285 kb on - strandat 2172.285 kb on - strandat 2172.295 kb on + strandat 2172.296 kb on - strandat 2172.303 kb on - strandat 2172.305 kb on - strandat 2172.315 kb on - strandat 2172.430 kb on - strand, within gatBat 2172.542 kb on - strand, within gatBat 2172.552 kb on - strand, within gatBat 2172.688 kb on - strand, within gatAat 2172.690 kb on - strand, within gatAat 2172.690 kb on - strand, within gatAat 2172.716 kb on - strand, within gatAat 2172.726 kb on - strand, within gatAat 2172.807 kb on - strand, within gatAat 2172.839 kb on - strand, within gatAat 2172.917 kb on - strand, within gatAat 2172.978 kb on - strand, within gatAat 2172.978 kb on - strand, within gatAat 2172.983 kb on + strand, within gatAat 2173.004 kb on - strand, within gatAat 2173.067 kb on + strandat 2173.075 kb on - strandat 2173.177 kb on - strandat 2173.177 kb on - strandat 2173.197 kb on - strandat 2173.197 kb on - strandat 2173.205 kb on - strandat 2173.205 kb on - strandat 2173.233 kb on - strand, within gatZat 2173.440 kb on - strand, within gatZat 2173.444 kb on - strand, within gatZat 2173.451 kb on + strand, within gatZat 2173.451 kb on + strand, within gatZat 2173.459 kb on - strand, within gatZat 2173.459 kb on - strand, within gatZat 2173.485 kb on - strand, within gatZat 2173.588 kb on - strand, within gatZat 2173.638 kb on - strand, within gatZat 2173.717 kb on - strand, within gatZat 2173.783 kb on + strand, within gatZat 2173.791 kb on - strand, within gatZat 2173.791 kb on - strand, within gatZat 2173.870 kb on - strand, within gatZat 2174.277 kb on + strandat 2174.277 kb on + strandat 2174.285 kb on - strandat 2174.285 kb on - strandat 2174.285 kb on - strandat 2174.371 kb on + strandat 2174.422 kb on - strandat 2174.422 kb on - strandat 2174.469 kb on + strand, within gatYat 2174.749 kb on + strand, within gatYat 2174.749 kb on + strand, within gatYat 2175.002 kb on + strand, within gatYat 2175.029 kb on + strand, within gatYat 2175.129 kb on + strand, within gatYat 2175.189 kb on + strandat 2175.226 kb on + strandat 2175.226 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Anaerobic with Cefoxitin 0.000003125 mM
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2,172,130 + gatC b2092 0.87 +0.8
2,172,137 - gatC b2092 0.88 +2.5
2,172,247 + -0.2
2,172,255 + +0.8
2,172,285 - +1.9
2,172,285 - +3.5
2,172,295 + +0.8
2,172,296 - +2.5
2,172,303 - +2.1
2,172,305 - +1.6
2,172,315 - +2.8
2,172,430 - gatB b2093 0.44 +1.8
2,172,542 - gatB b2093 0.84 +0.0
2,172,552 - gatB b2093 0.87 -0.2
2,172,688 - gatA b2094 0.15 +0.6
2,172,690 - gatA b2094 0.16 +0.6
2,172,690 - gatA b2094 0.16 -0.7
2,172,716 - gatA b2094 0.21 -0.2
2,172,726 - gatA b2094 0.24 +1.0
2,172,807 - gatA b2094 0.42 +0.1
2,172,839 - gatA b2094 0.49 +0.8
2,172,917 - gatA b2094 0.66 +0.9
2,172,978 - gatA b2094 0.79 +0.7
2,172,978 - gatA b2094 0.79 +2.7
2,172,983 + gatA b2094 0.80 +0.8
2,173,004 - gatA b2094 0.85 -0.4
2,173,067 + +0.8
2,173,075 - +0.3
2,173,177 - +0.5
2,173,177 - +0.9
2,173,197 - +2.0
2,173,197 - +1.8
2,173,205 - +2.4
2,173,205 - +0.4
2,173,233 - gatZ b2095 0.12 -0.9
2,173,440 - gatZ b2095 0.28 +1.6
2,173,444 - gatZ b2095 0.29 +1.6
2,173,451 + gatZ b2095 0.29 +0.0
2,173,451 + gatZ b2095 0.29 +3.9
2,173,459 - gatZ b2095 0.30 +1.2
2,173,459 - gatZ b2095 0.30 +1.2
2,173,485 - gatZ b2095 0.32 -0.2
2,173,588 - gatZ b2095 0.40 +1.6
2,173,638 - gatZ b2095 0.44 +2.0
2,173,717 - gatZ b2095 0.50 +1.5
2,173,783 + gatZ b2095 0.56 +3.8
2,173,791 - gatZ b2095 0.56 +0.6
2,173,791 - gatZ b2095 0.56 +1.0
2,173,870 - gatZ b2095 0.62 +1.2
2,174,277 + +2.6
2,174,277 + +3.4
2,174,285 - +2.4
2,174,285 - +2.9
2,174,285 - +3.7
2,174,371 + +3.3
2,174,422 - +0.8
2,174,422 - +0.2
2,174,469 + gatY b2096 0.11 +2.3
2,174,749 + gatY b2096 0.44 +3.2
2,174,749 + gatY b2096 0.44 +4.7
2,175,002 + gatY b2096 0.74 +4.0
2,175,029 + gatY b2096 0.77 +3.2
2,175,129 + gatY b2096 0.89 +3.4
2,175,189 + +3.2
2,175,226 + +0.8
2,175,226 + +3.4

Or see this region's nucleotide sequence