Experiment: LB Anaerobic with Mecillinam 0.0000078125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ispB and sfsB are separated by 227 nucleotides sfsB and murA are separated by 47 nucleotides murA and yrbA are separated by 54 nucleotides yrbA and yrbB are separated by 159 nucleotides yrbB and yrbC overlap by 1 nucleotides
b3187: ispB - octaprenyl diphosphate synthase (NCBI), at 3,331,732 to 3,332,703
ispB
b3188: sfsB - DNA-binding transcriptional activator of maltose metabolism (NCBI), at 3,332,931 to 3,333,209
sfsB
b3189: murA - UDP-N-acetylglucosamine 1-carboxyvinyltransferase (NCBI), at 3,333,257 to 3,334,516
murA
b3190: yrbA - orf, hypothetical protein (VIMSS), at 3,334,571 to 3,334,825
yrbA
b3191: yrbB - orf, hypothetical protein (VIMSS), at 3,334,985 to 3,335,278
yrbB
b3192: yrbC - predicted ABC-type organic solvent transporter (NCBI), at 3,335,278 to 3,335,913
yrbC
Position (kb)
3333
3334
3335 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3332.691 kb on - strand at 3332.718 kb on - strand at 3332.858 kb on - strand at 3332.858 kb on - strand at 3332.866 kb on + strand at 3332.913 kb on + strand at 3332.919 kb on - strand at 3333.104 kb on - strand, within sfsB at 3333.104 kb on - strand, within sfsB at 3333.144 kb on + strand, within sfsB at 3333.150 kb on + strand, within sfsB at 3333.151 kb on + strand, within sfsB at 3333.176 kb on + strand, within sfsB at 3333.190 kb on - strand at 3334.619 kb on - strand, within yrbA at 3334.630 kb on + strand, within yrbA at 3334.638 kb on - strand, within yrbA at 3334.638 kb on - strand, within yrbA at 3334.639 kb on + strand, within yrbA at 3334.639 kb on + strand, within yrbA at 3334.710 kb on - strand, within yrbA at 3334.718 kb on + strand, within yrbA at 3334.724 kb on + strand, within yrbA at 3334.780 kb on - strand, within yrbA at 3334.780 kb on - strand, within yrbA at 3334.793 kb on + strand, within yrbA at 3334.801 kb on - strand at 3334.801 kb on - strand at 3334.843 kb on + strand at 3334.883 kb on + strand at 3334.918 kb on + strand at 3334.918 kb on + strand at 3335.030 kb on + strand, within yrbB at 3335.030 kb on + strand, within yrbB at 3335.030 kb on + strand, within yrbB at 3335.030 kb on + strand, within yrbB at 3335.030 kb on + strand, within yrbB at 3335.030 kb on + strand, within yrbB at 3335.031 kb on + strand, within yrbB at 3335.031 kb on + strand, within yrbB at 3335.036 kb on + strand, within yrbB at 3335.036 kb on + strand, within yrbB at 3335.038 kb on - strand, within yrbB at 3335.038 kb on - strand, within yrbB at 3335.038 kb on - strand, within yrbB at 3335.038 kb on - strand, within yrbB at 3335.038 kb on - strand, within yrbB at 3335.059 kb on - strand, within yrbB at 3335.060 kb on - strand, within yrbB at 3335.098 kb on + strand, within yrbB at 3335.098 kb on + strand, within yrbB at 3335.102 kb on + strand, within yrbB at 3335.102 kb on + strand, within yrbB at 3335.120 kb on + strand, within yrbB at 3335.128 kb on - strand, within yrbB at 3335.137 kb on + strand, within yrbB at 3335.142 kb on + strand, within yrbB at 3335.142 kb on + strand, within yrbB at 3335.162 kb on - strand, within yrbB at 3335.165 kb on + strand, within yrbB at 3335.198 kb on + strand, within yrbB at 3335.198 kb on + strand, within yrbB at 3335.205 kb on + strand, within yrbB at 3335.236 kb on - strand, within yrbB at 3335.340 kb on + strand at 3335.362 kb on - strand, within yrbC at 3335.362 kb on - strand, within yrbC at 3335.404 kb on + strand, within yrbC at 3335.428 kb on - strand, within yrbC at 3335.428 kb on - strand, within yrbC at 3335.461 kb on + strand, within yrbC at 3335.481 kb on + strand, within yrbC at 3335.500 kb on + strand, within yrbC at 3335.500 kb on + strand, within yrbC
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Mecillinam 0.0000078125 mM remove 3,332,691 - -0.2 3,332,718 - +0.2 3,332,858 - +0.1 3,332,858 - -1.0 3,332,866 + +0.3 3,332,913 + +0.0 3,332,919 - +0.6 3,333,104 - sfsB b3188 0.62 +0.4 3,333,104 - sfsB b3188 0.62 -0.7 3,333,144 + sfsB b3188 0.76 +0.6 3,333,150 + sfsB b3188 0.78 -0.4 3,333,151 + sfsB b3188 0.79 -0.1 3,333,176 + sfsB b3188 0.88 -1.1 3,333,190 - +0.3 3,334,619 - yrbA b3190 0.19 +0.3 3,334,630 + yrbA b3190 0.23 -0.8 3,334,638 - yrbA b3190 0.26 +0.5 3,334,638 - yrbA b3190 0.26 -0.3 3,334,639 + yrbA b3190 0.27 +0.7 3,334,639 + yrbA b3190 0.27 +1.0 3,334,710 - yrbA b3190 0.55 +0.3 3,334,718 + yrbA b3190 0.58 +0.7 3,334,724 + yrbA b3190 0.60 +1.1 3,334,780 - yrbA b3190 0.82 +1.3 3,334,780 - yrbA b3190 0.82 +1.1 3,334,793 + yrbA b3190 0.87 -2.5 3,334,801 - +1.1 3,334,801 - -0.7 3,334,843 + -0.9 3,334,883 + -0.5 3,334,918 + -0.9 3,334,918 + +0.0 3,335,030 + yrbB b3191 0.15 +0.1 3,335,030 + yrbB b3191 0.15 -0.4 3,335,030 + yrbB b3191 0.15 +0.4 3,335,030 + yrbB b3191 0.15 -0.0 3,335,030 + yrbB b3191 0.15 -0.1 3,335,030 + yrbB b3191 0.15 -1.5 3,335,031 + yrbB b3191 0.16 +0.8 3,335,031 + yrbB b3191 0.16 +1.2 3,335,036 + yrbB b3191 0.17 -1.3 3,335,036 + yrbB b3191 0.17 +0.3 3,335,038 - yrbB b3191 0.18 +1.5 3,335,038 - yrbB b3191 0.18 +0.4 3,335,038 - yrbB b3191 0.18 +0.7 3,335,038 - yrbB b3191 0.18 -0.3 3,335,038 - yrbB b3191 0.18 +0.2 3,335,059 - yrbB b3191 0.25 +1.0 3,335,060 - yrbB b3191 0.26 -1.4 3,335,098 + yrbB b3191 0.38 -0.2 3,335,098 + yrbB b3191 0.38 +0.5 3,335,102 + yrbB b3191 0.40 -0.1 3,335,102 + yrbB b3191 0.40 -0.1 3,335,120 + yrbB b3191 0.46 -0.6 3,335,128 - yrbB b3191 0.49 +0.1 3,335,137 + yrbB b3191 0.52 -0.6 3,335,142 + yrbB b3191 0.53 +0.1 3,335,142 + yrbB b3191 0.53 -0.6 3,335,162 - yrbB b3191 0.60 -0.8 3,335,165 + yrbB b3191 0.61 +0.4 3,335,198 + yrbB b3191 0.72 -0.8 3,335,198 + yrbB b3191 0.72 +0.1 3,335,205 + yrbB b3191 0.75 +2.6 3,335,236 - yrbB b3191 0.85 +0.8 3,335,340 + -0.4 3,335,362 - yrbC b3192 0.13 -0.4 3,335,362 - yrbC b3192 0.13 +0.1 3,335,404 + yrbC b3192 0.20 -0.5 3,335,428 - yrbC b3192 0.24 +1.0 3,335,428 - yrbC b3192 0.24 -1.3 3,335,461 + yrbC b3192 0.29 -0.2 3,335,481 + yrbC b3192 0.32 +0.2 3,335,500 + yrbC b3192 0.35 +0.2 3,335,500 + yrbC b3192 0.35 -0.3
Or see this region's nucleotide sequence