Strain Fitness in Escherichia coli BW25113 around b2175

Experiment: LB Anaerobic with Mecillinam 0.0000078125 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntb2174 and spr are separated by 411 nucleotidesspr and rtn are separated by 180 nucleotides b2174: b2174 - orf, hypothetical protein (VIMSS), at 2,266,876 to 2,267,589 b2174 b2175: spr - predicted peptidase, outer membrane lipoprotein (NCBI), at 2,268,001 to 2,268,567 spr b2176: rtn - hypothetical protein (NCBI), at 2,268,748 to 2,270,304 rtn Position (kb) 2268 2269Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 2267.048 kb on - strand, within b2174at 2267.073 kb on - strand, within b2174at 2267.090 kb on - strand, within b2174at 2267.090 kb on - strand, within b2174at 2267.171 kb on - strand, within b2174at 2267.174 kb on + strand, within b2174at 2267.185 kb on + strand, within b2174at 2267.185 kb on + strand, within b2174at 2267.185 kb on + strand, within b2174at 2267.185 kb on + strand, within b2174at 2267.231 kb on + strand, within b2174at 2267.231 kb on + strand, within b2174at 2267.323 kb on - strand, within b2174at 2267.428 kb on + strand, within b2174at 2267.457 kb on - strand, within b2174at 2267.490 kb on - strand, within b2174at 2267.496 kb on + strand, within b2174at 2267.496 kb on + strand, within b2174at 2267.496 kb on + strand, within b2174at 2267.497 kb on + strand, within b2174at 2267.515 kb on + strand, within b2174at 2267.515 kb on + strand, within b2174at 2267.522 kb on - strandat 2267.523 kb on - strandat 2267.535 kb on - strandat 2267.618 kb on + strandat 2267.628 kb on - strandat 2267.628 kb on - strandat 2267.638 kb on - strandat 2267.638 kb on - strandat 2267.644 kb on - strandat 2267.644 kb on - strandat 2267.645 kb on + strandat 2267.659 kb on - strandat 2267.659 kb on - strandat 2267.661 kb on - strandat 2267.793 kb on - strandat 2267.806 kb on - strandat 2267.817 kb on + strandat 2267.819 kb on - strandat 2267.853 kb on + strandat 2268.026 kb on + strandat 2268.026 kb on + strandat 2268.056 kb on + strandat 2268.058 kb on - strand, within sprat 2268.072 kb on - strand, within sprat 2268.142 kb on - strand, within sprat 2268.216 kb on + strand, within sprat 2268.216 kb on + strand, within sprat 2268.216 kb on + strand, within sprat 2268.306 kb on - strand, within sprat 2268.416 kb on - strand, within sprat 2268.468 kb on - strand, within sprat 2268.516 kb on + strandat 2268.516 kb on + strandat 2268.536 kb on - strandat 2268.574 kb on - strandat 2268.574 kb on - strandat 2268.579 kb on + strandat 2268.594 kb on - strandat 2268.660 kb on + strandat 2268.660 kb on + strandat 2268.684 kb on + strandat 2268.694 kb on - strandat 2268.694 kb on - strandat 2268.813 kb on - strandat 2268.813 kb on - strandat 2268.895 kb on + strandat 2268.895 kb on + strandat 2268.933 kb on + strand, within rtnat 2268.933 kb on + strand, within rtnat 2268.971 kb on - strand, within rtnat 2269.018 kb on - strand, within rtnat 2269.083 kb on - strand, within rtnat 2269.083 kb on - strand, within rtnat 2269.086 kb on + strand, within rtnat 2269.147 kb on + strand, within rtnat 2269.147 kb on + strand, within rtnat 2269.147 kb on + strand, within rtnat 2269.155 kb on - strand, within rtnat 2269.155 kb on - strand, within rtnat 2269.338 kb on + strand, within rtnat 2269.358 kb on + strand, within rtnat 2269.358 kb on + strand, within rtnat 2269.415 kb on - strand, within rtnat 2269.425 kb on - strand, within rtnat 2269.447 kb on - strand, within rtnat 2269.447 kb on - strand, within rtnat 2269.511 kb on - strand, within rtnat 2269.511 kb on - strand, within rtnat 2269.516 kb on - strand, within rtn

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Anaerobic with Mecillinam 0.0000078125 mM
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2,267,048 - b2174 b2174 0.24 +0.1
2,267,073 - b2174 b2174 0.28 -0.9
2,267,090 - b2174 b2174 0.30 -0.6
2,267,090 - b2174 b2174 0.30 +0.0
2,267,171 - b2174 b2174 0.41 +0.9
2,267,174 + b2174 b2174 0.42 +0.5
2,267,185 + b2174 b2174 0.43 -0.5
2,267,185 + b2174 b2174 0.43 -0.4
2,267,185 + b2174 b2174 0.43 -1.0
2,267,185 + b2174 b2174 0.43 +0.5
2,267,231 + b2174 b2174 0.50 -0.3
2,267,231 + b2174 b2174 0.50 -0.3
2,267,323 - b2174 b2174 0.63 +0.8
2,267,428 + b2174 b2174 0.77 +1.6
2,267,457 - b2174 b2174 0.81 -0.3
2,267,490 - b2174 b2174 0.86 -0.6
2,267,496 + b2174 b2174 0.87 -0.9
2,267,496 + b2174 b2174 0.87 -0.8
2,267,496 + b2174 b2174 0.87 -0.9
2,267,497 + b2174 b2174 0.87 -0.3
2,267,515 + b2174 b2174 0.89 -0.4
2,267,515 + b2174 b2174 0.89 -0.4
2,267,522 - +0.5
2,267,523 - +1.1
2,267,535 - -0.6
2,267,618 + -0.1
2,267,628 - -0.2
2,267,628 - +1.9
2,267,638 - -0.3
2,267,638 - -2.0
2,267,644 - +0.0
2,267,644 - -0.8
2,267,645 + +0.4
2,267,659 - -2.0
2,267,659 - +0.7
2,267,661 - +1.9
2,267,793 - -0.6
2,267,806 - -1.2
2,267,817 + +0.3
2,267,819 - -0.9
2,267,853 + +0.6
2,268,026 + -0.2
2,268,026 + -3.6
2,268,056 + -2.4
2,268,058 - spr b2175 0.10 -3.6
2,268,072 - spr b2175 0.13 -2.6
2,268,142 - spr b2175 0.25 -2.0
2,268,216 + spr b2175 0.38 -3.8
2,268,216 + spr b2175 0.38 -0.6
2,268,216 + spr b2175 0.38 -2.2
2,268,306 - spr b2175 0.54 -3.6
2,268,416 - spr b2175 0.73 -1.2
2,268,468 - spr b2175 0.82 -0.6
2,268,516 + -3.3
2,268,516 + -1.6
2,268,536 - +0.4
2,268,574 - +0.4
2,268,574 - +0.4
2,268,579 + -0.5
2,268,594 - -0.3
2,268,660 + +0.9
2,268,660 + +0.9
2,268,684 + +0.6
2,268,694 - -1.7
2,268,694 - -0.2
2,268,813 - -1.7
2,268,813 - -0.1
2,268,895 + +0.4
2,268,895 + -0.1
2,268,933 + rtn b2176 0.12 +0.0
2,268,933 + rtn b2176 0.12 +0.7
2,268,971 - rtn b2176 0.14 +0.4
2,269,018 - rtn b2176 0.17 +0.6
2,269,083 - rtn b2176 0.22 +1.0
2,269,083 - rtn b2176 0.22 -2.0
2,269,086 + rtn b2176 0.22 +0.1
2,269,147 + rtn b2176 0.26 -0.2
2,269,147 + rtn b2176 0.26 +0.4
2,269,147 + rtn b2176 0.26 -0.8
2,269,155 - rtn b2176 0.26 +0.9
2,269,155 - rtn b2176 0.26 -0.8
2,269,338 + rtn b2176 0.38 -0.4
2,269,358 + rtn b2176 0.39 +0.4
2,269,358 + rtn b2176 0.39 -1.0
2,269,415 - rtn b2176 0.43 +0.4
2,269,425 - rtn b2176 0.43 -0.3
2,269,447 - rtn b2176 0.45 -0.1
2,269,447 - rtn b2176 0.45 -1.1
2,269,511 - rtn b2176 0.49 +0.2
2,269,511 - rtn b2176 0.49 +0.6
2,269,516 - rtn b2176 0.49 -2.4

Or see this region's nucleotide sequence