Experiment: 72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 30% DO with 20% v/v oleyl alcohol overlay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_4928 and PP_4929 are separated by 11 nucleotides PP_4929 and PP_4930 are separated by 71 nucleotides PP_4930 and PP_4931 are separated by 88 nucleotides
PP_4928: PP_4928 - 3-deoxy-D-manno-octulosonic acid transferase, at 5,608,326 to 5,609,597
_4928
PP_4929: PP_4929 - Transcriptional regulator, LysR family, at 5,609,609 to 5,610,514
_4929
PP_4930: PP_4930 - putative small multidrug resistance protein, at 5,610,586 to 5,610,918
_4930
PP_4931: PP_4931 - conserved protein of unknown function, at 5,611,007 to 5,612,182
_4931
Position (kb) 5609
5610
5611 Strain fitness (log2 ratio) -2
-1
0
1
2 at 5609.582 kb on - strand at 5609.605 kb on - strand at 5609.754 kb on + strand, within PP_4929 at 5609.754 kb on + strand, within PP_4929 at 5609.754 kb on + strand, within PP_4929 at 5609.755 kb on - strand, within PP_4929 at 5609.763 kb on + strand, within PP_4929 at 5609.829 kb on + strand, within PP_4929 at 5609.829 kb on + strand, within PP_4929 at 5609.850 kb on - strand, within PP_4929 at 5610.553 kb on + strand at 5610.553 kb on + strand at 5610.553 kb on + strand at 5610.553 kb on + strand at 5610.554 kb on - strand at 5610.595 kb on + strand at 5610.596 kb on - strand at 5610.597 kb on + strand at 5610.597 kb on + strand at 5610.597 kb on + strand at 5610.597 kb on + strand at 5610.597 kb on + strand at 5610.598 kb on - strand at 5610.598 kb on - strand at 5610.598 kb on - strand at 5610.598 kb on - strand at 5610.598 kb on - strand at 5610.598 kb on - strand at 5610.598 kb on - strand at 5610.601 kb on + strand at 5610.602 kb on - strand at 5610.704 kb on - strand, within PP_4930 at 5610.764 kb on - strand, within PP_4930 at 5610.820 kb on + strand, within PP_4930 at 5610.821 kb on - strand, within PP_4930 at 5610.821 kb on - strand, within PP_4930 at 5610.837 kb on + strand, within PP_4930 at 5610.837 kb on + strand, within PP_4930 at 5610.961 kb on + strand at 5610.962 kb on - strand at 5610.962 kb on - strand at 5610.963 kb on + strand at 5610.963 kb on + strand at 5610.963 kb on + strand at 5610.964 kb on - strand at 5610.964 kb on - strand at 5610.970 kb on + strand at 5610.970 kb on + strand at 5610.970 kb on + strand at 5610.971 kb on - strand at 5610.971 kb on - strand at 5610.972 kb on + strand at 5610.972 kb on + strand at 5610.972 kb on + strand at 5610.972 kb on + strand at 5610.972 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 30% DO with 20% v/v oleyl alcohol overlay remove 5,609,582 - +0.9 5,609,605 - +0.7 5,609,754 + PP_4929 0.16 -0.4 5,609,754 + PP_4929 0.16 +0.9 5,609,754 + PP_4929 0.16 -0.5 5,609,755 - PP_4929 0.16 -0.3 5,609,763 + PP_4929 0.17 +1.7 5,609,829 + PP_4929 0.24 -0.2 5,609,829 + PP_4929 0.24 -1.2 5,609,850 - PP_4929 0.27 -0.3 5,610,553 + -0.8 5,610,553 + +0.2 5,610,553 + -1.4 5,610,553 + +0.9 5,610,554 - +1.9 5,610,595 + -0.7 5,610,596 - -0.7 5,610,597 + +0.3 5,610,597 + +0.2 5,610,597 + +0.9 5,610,597 + +0.7 5,610,597 + +0.7 5,610,598 - -1.2 5,610,598 - +0.7 5,610,598 - +0.3 5,610,598 - -0.1 5,610,598 - +0.7 5,610,598 - +0.2 5,610,598 - -0.7 5,610,601 + +0.7 5,610,602 - +0.9 5,610,704 - PP_4930 0.35 -1.1 5,610,764 - PP_4930 0.53 -0.7 5,610,820 + PP_4930 0.70 -0.5 5,610,821 - PP_4930 0.71 -0.3 5,610,821 - PP_4930 0.71 -0.7 5,610,837 + PP_4930 0.75 -0.2 5,610,837 + PP_4930 0.75 -0.1 5,610,961 + -2.4 5,610,962 - +0.4 5,610,962 - -1.1 5,610,963 + +0.2 5,610,963 + -0.4 5,610,963 + +0.8 5,610,964 - -0.5 5,610,964 - +0.4 5,610,970 + +0.6 5,610,970 + -0.6 5,610,970 + -0.1 5,610,971 - -0.7 5,610,971 - -1.1 5,610,972 + -0.7 5,610,972 + +0.1 5,610,972 + +0.9 5,610,972 + -0.2 5,610,972 + +0.5
Or see this region's nucleotide sequence