Experiment: 72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 30% DO with 20% v/v oleyl alcohol overlay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_4927 and PP_4928 are separated by 187 nucleotides PP_4928 and PP_4929 are separated by 11 nucleotides PP_4929 and PP_4930 are separated by 71 nucleotides
PP_4927: PP_4927 - Cyclolysin secretion/processing ATP-binding protein CyaB, at 5,605,943 to 5,608,138
_4927
PP_4928: PP_4928 - 3-deoxy-D-manno-octulosonic acid transferase, at 5,608,326 to 5,609,597
_4928
PP_4929: PP_4929 - Transcriptional regulator, LysR family, at 5,609,609 to 5,610,514
_4929
PP_4930: PP_4930 - putative small multidrug resistance protein, at 5,610,586 to 5,610,918
_4930
Position (kb) 5608
5609
5610 Strain fitness (log2 ratio) -2
-1
0
1
2 at 5607.424 kb on + strand, within PP_4927 at 5607.425 kb on - strand, within PP_4927 at 5607.425 kb on - strand, within PP_4927 at 5607.438 kb on + strand, within PP_4927 at 5607.439 kb on - strand, within PP_4927 at 5607.439 kb on - strand, within PP_4927 at 5607.439 kb on - strand, within PP_4927 at 5607.442 kb on + strand, within PP_4927 at 5607.442 kb on + strand, within PP_4927 at 5607.442 kb on + strand, within PP_4927 at 5607.443 kb on - strand, within PP_4927 at 5607.545 kb on + strand, within PP_4927 at 5607.546 kb on - strand, within PP_4927 at 5607.631 kb on - strand, within PP_4927 at 5607.659 kb on - strand, within PP_4927 at 5607.659 kb on - strand, within PP_4927 at 5607.825 kb on + strand, within PP_4927 at 5607.826 kb on - strand, within PP_4927 at 5607.838 kb on + strand, within PP_4927 at 5607.839 kb on - strand, within PP_4927 at 5607.851 kb on - strand, within PP_4927 at 5607.935 kb on + strand at 5607.936 kb on - strand at 5608.101 kb on - strand at 5608.127 kb on - strand at 5608.140 kb on - strand at 5608.260 kb on - strand at 5608.285 kb on + strand at 5608.285 kb on + strand at 5608.286 kb on - strand at 5608.315 kb on + strand at 5608.316 kb on - strand at 5608.316 kb on - strand at 5608.324 kb on + strand at 5609.582 kb on - strand at 5609.605 kb on - strand at 5609.754 kb on + strand, within PP_4929 at 5609.754 kb on + strand, within PP_4929 at 5609.754 kb on + strand, within PP_4929 at 5609.755 kb on - strand, within PP_4929 at 5609.763 kb on + strand, within PP_4929 at 5609.829 kb on + strand, within PP_4929 at 5609.829 kb on + strand, within PP_4929 at 5609.850 kb on - strand, within PP_4929 at 5610.553 kb on + strand at 5610.553 kb on + strand at 5610.553 kb on + strand at 5610.553 kb on + strand at 5610.554 kb on - strand at 5610.595 kb on + strand at 5610.596 kb on - strand at 5610.597 kb on + strand at 5610.597 kb on + strand at 5610.597 kb on + strand at 5610.597 kb on + strand at 5610.597 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 30% DO with 20% v/v oleyl alcohol overlay remove 5,607,424 + PP_4927 0.67 -0.7 5,607,425 - PP_4927 0.67 -0.5 5,607,425 - PP_4927 0.67 -0.6 5,607,438 + PP_4927 0.68 +0.7 5,607,439 - PP_4927 0.68 -1.6 5,607,439 - PP_4927 0.68 -0.6 5,607,439 - PP_4927 0.68 -1.6 5,607,442 + PP_4927 0.68 -1.1 5,607,442 + PP_4927 0.68 +0.2 5,607,442 + PP_4927 0.68 -0.4 5,607,443 - PP_4927 0.68 +0.9 5,607,545 + PP_4927 0.73 -0.4 5,607,546 - PP_4927 0.73 -0.6 5,607,631 - PP_4927 0.77 +1.9 5,607,659 - PP_4927 0.78 -0.2 5,607,659 - PP_4927 0.78 -0.4 5,607,825 + PP_4927 0.86 -0.5 5,607,826 - PP_4927 0.86 -0.1 5,607,838 + PP_4927 0.86 +1.1 5,607,839 - PP_4927 0.86 -0.9 5,607,851 - PP_4927 0.87 -0.3 5,607,935 + +0.9 5,607,936 - -0.2 5,608,101 - -0.5 5,608,127 - -0.6 5,608,140 - -1.0 5,608,260 - +1.5 5,608,285 + +0.9 5,608,285 + -0.5 5,608,286 - +0.5 5,608,315 + -0.1 5,608,316 - +0.6 5,608,316 - -1.8 5,608,324 + +0.3 5,609,582 - +0.9 5,609,605 - +0.7 5,609,754 + PP_4929 0.16 -0.4 5,609,754 + PP_4929 0.16 +0.9 5,609,754 + PP_4929 0.16 -0.5 5,609,755 - PP_4929 0.16 -0.3 5,609,763 + PP_4929 0.17 +1.7 5,609,829 + PP_4929 0.24 -0.2 5,609,829 + PP_4929 0.24 -1.2 5,609,850 - PP_4929 0.27 -0.3 5,610,553 + -0.8 5,610,553 + +0.2 5,610,553 + -1.4 5,610,553 + +0.9 5,610,554 - +1.9 5,610,595 + -0.7 5,610,596 - -0.7 5,610,597 + +0.3 5,610,597 + +0.2 5,610,597 + +0.9 5,610,597 + +0.7 5,610,597 + +0.7
Or see this region's nucleotide sequence