Experiment: 72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 30% DO with 20% v/v oleyl alcohol overlay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_4843 and PP_4844 overlap by 4 nucleotides PP_4844 and PP_4845 are separated by 153 nucleotides PP_4845 and PP_4846 are separated by 31 nucleotides
PP_4843: PP_4843 - putative Urea ABC transporter, permease protein, at 5,511,289 to 5,512,368
_4843
PP_4844: PP_4844 - putative Urea ABC transporter, ATP-binding protein, at 5,512,365 to 5,513,222
_4844
PP_4845: PP_4845 - putative Urea ABC transporter, ATP-binding protein, at 5,513,376 to 5,514,074
_4845
PP_4846: PP_4846 - L-amino acid N-acyltransferase, at 5,514,106 to 5,514,621
_4846
Position (kb) 5512
5513
5514 Strain fitness (log2 ratio) -2
-1
0
1
2 at 5511.433 kb on + strand, within PP_4843 at 5511.434 kb on - strand, within PP_4843 at 5511.434 kb on - strand, within PP_4843 at 5511.466 kb on + strand, within PP_4843 at 5511.571 kb on + strand, within PP_4843 at 5511.571 kb on + strand, within PP_4843 at 5511.571 kb on + strand at 5511.572 kb on - strand, within PP_4843 at 5511.647 kb on - strand, within PP_4843 at 5511.760 kb on + strand, within PP_4843 at 5511.761 kb on - strand, within PP_4843 at 5511.946 kb on + strand, within PP_4843 at 5511.946 kb on + strand, within PP_4843 at 5512.033 kb on + strand, within PP_4843 at 5512.034 kb on - strand, within PP_4843 at 5512.106 kb on - strand, within PP_4843 at 5512.318 kb on + strand at 5512.318 kb on + strand at 5512.318 kb on + strand at 5512.318 kb on + strand at 5512.560 kb on + strand, within PP_4844 at 5512.561 kb on - strand, within PP_4844 at 5512.631 kb on - strand, within PP_4844 at 5512.631 kb on - strand, within PP_4844 at 5512.631 kb on - strand, within PP_4844 at 5512.668 kb on + strand, within PP_4844 at 5512.669 kb on - strand, within PP_4844 at 5512.707 kb on + strand, within PP_4844 at 5513.208 kb on + strand at 5513.209 kb on - strand at 5513.209 kb on - strand at 5513.277 kb on + strand at 5513.277 kb on + strand at 5513.278 kb on - strand at 5513.278 kb on - strand at 5513.288 kb on + strand at 5513.288 kb on + strand at 5513.289 kb on - strand at 5513.314 kb on - strand at 5513.325 kb on + strand at 5513.403 kb on + strand at 5513.403 kb on + strand at 5513.403 kb on + strand at 5513.404 kb on - strand at 5513.406 kb on + strand at 5513.406 kb on + strand at 5513.407 kb on - strand at 5513.407 kb on - strand at 5513.407 kb on - strand at 5513.613 kb on + strand, within PP_4845 at 5513.613 kb on + strand, within PP_4845 at 5513.613 kb on + strand, within PP_4845 at 5513.725 kb on - strand, within PP_4845 at 5513.725 kb on - strand, within PP_4845 at 5513.955 kb on + strand, within PP_4845 at 5513.956 kb on - strand, within PP_4845 at 5514.052 kb on + strand at 5514.052 kb on + strand at 5514.052 kb on + strand at 5514.053 kb on - strand at 5514.053 kb on - strand at 5514.064 kb on + strand at 5514.072 kb on + strand at 5514.073 kb on - strand at 5514.152 kb on + strand at 5514.163 kb on + strand, within PP_4846 at 5514.164 kb on - strand, within PP_4846 at 5514.164 kb on - strand, within PP_4846 at 5514.164 kb on - strand, within PP_4846 at 5514.164 kb on - strand, within PP_4846
Per-strain Table
Position Strand Gene LocusTag Fraction 72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 30% DO with 20% v/v oleyl alcohol overlay remove 5,511,433 + PP_4843 0.13 -1.4 5,511,434 - PP_4843 0.13 -0.0 5,511,434 - PP_4843 0.13 +0.6 5,511,466 + PP_4843 0.16 -0.5 5,511,571 + PP_4843 0.26 -1.1 5,511,571 + PP_4843 0.26 -0.8 5,511,571 + +1.0 5,511,572 - PP_4843 0.26 -1.0 5,511,647 - PP_4843 0.33 +1.8 5,511,760 + PP_4843 0.44 -0.6 5,511,761 - PP_4843 0.44 -0.0 5,511,946 + PP_4843 0.61 -2.2 5,511,946 + PP_4843 0.61 -0.0 5,512,033 + PP_4843 0.69 +1.4 5,512,034 - PP_4843 0.69 -0.6 5,512,106 - PP_4843 0.76 -0.6 5,512,318 + -0.2 5,512,318 + +2.0 5,512,318 + -1.6 5,512,318 + +1.1 5,512,560 + PP_4844 0.23 +1.8 5,512,561 - PP_4844 0.23 +0.3 5,512,631 - PP_4844 0.31 +2.2 5,512,631 - PP_4844 0.31 +0.4 5,512,631 - PP_4844 0.31 +1.2 5,512,668 + PP_4844 0.35 -1.0 5,512,669 - PP_4844 0.35 +0.8 5,512,707 + PP_4844 0.40 -0.6 5,513,208 + +0.2 5,513,209 - +1.0 5,513,209 - +0.3 5,513,277 + -0.2 5,513,277 + -0.2 5,513,278 - +0.9 5,513,278 - -0.1 5,513,288 + -0.1 5,513,288 + +1.4 5,513,289 - -1.1 5,513,314 - -0.4 5,513,325 + -0.0 5,513,403 + -0.3 5,513,403 + +0.9 5,513,403 + -0.6 5,513,404 - +0.4 5,513,406 + -1.5 5,513,406 + -0.1 5,513,407 - +0.5 5,513,407 - -1.6 5,513,407 - -1.6 5,513,613 + PP_4845 0.34 +0.9 5,513,613 + PP_4845 0.34 -0.9 5,513,613 + PP_4845 0.34 +0.7 5,513,725 - PP_4845 0.50 +0.4 5,513,725 - PP_4845 0.50 +0.8 5,513,955 + PP_4845 0.83 -0.6 5,513,956 - PP_4845 0.83 +0.5 5,514,052 + -0.1 5,514,052 + -0.1 5,514,052 + -1.1 5,514,053 - -0.8 5,514,053 - +0.9 5,514,064 + -0.1 5,514,072 + +0.8 5,514,073 - +0.4 5,514,152 + -0.2 5,514,163 + PP_4846 0.11 -0.2 5,514,164 - PP_4846 0.11 -0.1 5,514,164 - PP_4846 0.11 +0.9 5,514,164 - PP_4846 0.11 -0.1 5,514,164 - PP_4846 0.11 -1.7
Or see this region's nucleotide sequence