Experiment: 72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 30% DO with 20% v/v oleyl alcohol overlay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_1315 and PP_1316 are separated by 14 nucleotides PP_1316 and PP_1317 are separated by 262 nucleotides PP_1317 and PP_1318 overlap by 1 nucleotides
PP_1315: PP_1315 - 50S ribosomal protein L13, at 1,503,198 to 1,503,626
_1315
PP_1316: PP_1316 - 30S ribosomal protein S9, at 1,503,641 to 1,504,033
_1316
PP_1317: PP_1317 - Ubiquinol-cytochrome c reductase iron-sulfur subunit, at 1,504,296 to 1,504,889
_1317
PP_1318: PP_1318 - Cytochrome b, at 1,504,889 to 1,506,100
_1318
Position (kb) 1504
1505 Strain fitness (log2 ratio) -2
-1
0
1
2 at 1504.099 kb on - strand at 1504.131 kb on + strand at 1504.131 kb on + strand at 1504.137 kb on + strand at 1504.137 kb on + strand at 1504.138 kb on - strand at 1504.138 kb on - strand at 1504.175 kb on + strand at 1504.208 kb on - strand at 1504.208 kb on - strand at 1504.245 kb on + strand at 1504.245 kb on + strand at 1504.245 kb on + strand at 1504.245 kb on + strand at 1504.245 kb on + strand at 1504.246 kb on - strand at 1504.246 kb on - strand at 1504.246 kb on - strand at 1504.511 kb on - strand, within PP_1317 at 1504.608 kb on + strand, within PP_1317 at 1504.609 kb on - strand, within PP_1317 at 1504.609 kb on - strand, within PP_1317 at 1504.735 kb on + strand, within PP_1317 at 1504.735 kb on + strand, within PP_1317 at 1504.736 kb on - strand, within PP_1317 at 1504.736 kb on - strand, within PP_1317 at 1504.743 kb on + strand, within PP_1317 at 1504.770 kb on + strand, within PP_1317 at 1504.791 kb on + strand, within PP_1317 at 1504.791 kb on + strand, within PP_1317 at 1504.792 kb on - strand, within PP_1317 at 1504.839 kb on + strand at 1504.839 kb on + strand at 1504.840 kb on - strand at 1504.961 kb on + strand at 1504.961 kb on + strand at 1504.961 kb on + strand at 1504.961 kb on + strand at 1504.962 kb on - strand at 1504.962 kb on - strand at 1504.962 kb on - strand at 1504.964 kb on + strand at 1504.964 kb on + strand at 1504.964 kb on + strand at 1504.965 kb on - strand at 1505.102 kb on + strand, within PP_1318 at 1505.103 kb on - strand, within PP_1318 at 1505.124 kb on - strand, within PP_1318 at 1505.184 kb on - strand, within PP_1318 at 1505.210 kb on + strand, within PP_1318 at 1505.211 kb on - strand, within PP_1318 at 1505.264 kb on + strand, within PP_1318 at 1505.297 kb on + strand, within PP_1318 at 1505.297 kb on + strand, within PP_1318 at 1505.324 kb on + strand, within PP_1318 at 1505.324 kb on + strand, within PP_1318 at 1505.325 kb on - strand, within PP_1318 at 1505.408 kb on + strand, within PP_1318 at 1505.408 kb on + strand, within PP_1318 at 1505.409 kb on - strand, within PP_1318 at 1505.587 kb on + strand, within PP_1318 at 1505.603 kb on + strand, within PP_1318 at 1505.603 kb on + strand, within PP_1318 at 1505.604 kb on - strand, within PP_1318 at 1505.606 kb on + strand, within PP_1318 at 1505.607 kb on - strand, within PP_1318 at 1505.607 kb on - strand, within PP_1318 at 1505.690 kb on + strand, within PP_1318 at 1505.690 kb on + strand, within PP_1318 at 1505.778 kb on - strand, within PP_1318 at 1505.778 kb on - strand, within PP_1318
Per-strain Table
Position Strand Gene LocusTag Fraction 72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 30% DO with 20% v/v oleyl alcohol overlay remove 1,504,099 - -1.0 1,504,131 + -0.2 1,504,131 + -2.0 1,504,137 + +0.5 1,504,137 + +0.9 1,504,138 - -0.0 1,504,138 - +1.0 1,504,175 + -0.0 1,504,208 - +1.0 1,504,208 - +1.0 1,504,245 + -0.0 1,504,245 + -0.2 1,504,245 + +1.0 1,504,245 + +1.0 1,504,245 + +1.0 1,504,246 - +1.0 1,504,246 - +1.0 1,504,246 - +1.0 1,504,511 - PP_1317 0.36 +1.0 1,504,608 + PP_1317 0.53 +1.0 1,504,609 - PP_1317 0.53 -0.0 1,504,609 - PP_1317 0.53 +1.0 1,504,735 + PP_1317 0.74 +2.0 1,504,735 + PP_1317 0.74 +2.0 1,504,736 - PP_1317 0.74 +1.0 1,504,736 - PP_1317 0.74 -1.0 1,504,743 + PP_1317 0.75 -1.0 1,504,770 + PP_1317 0.80 +1.0 1,504,791 + PP_1317 0.83 +1.0 1,504,791 + PP_1317 0.83 -0.0 1,504,792 - PP_1317 0.84 -0.0 1,504,839 + +1.0 1,504,839 + -0.6 1,504,840 - +1.0 1,504,961 + +1.0 1,504,961 + +1.0 1,504,961 + +1.0 1,504,961 + +1.0 1,504,962 - +0.4 1,504,962 - +2.0 1,504,962 - -1.0 1,504,964 + +1.0 1,504,964 + +1.0 1,504,964 + -1.0 1,504,965 - +1.6 1,505,102 + PP_1318 0.18 +1.0 1,505,103 - PP_1318 0.18 -0.0 1,505,124 - PP_1318 0.19 -0.0 1,505,184 - PP_1318 0.24 +1.0 1,505,210 + PP_1318 0.26 +1.0 1,505,211 - PP_1318 0.27 -0.0 1,505,264 + PP_1318 0.31 +1.0 1,505,297 + PP_1318 0.34 -0.0 1,505,297 + PP_1318 0.34 -0.0 1,505,324 + PP_1318 0.36 -0.6 1,505,324 + PP_1318 0.36 -0.0 1,505,325 - PP_1318 0.36 +2.0 1,505,408 + PP_1318 0.43 +1.0 1,505,408 + PP_1318 0.43 -1.0 1,505,409 - PP_1318 0.43 +0.4 1,505,587 + PP_1318 0.58 +1.0 1,505,603 + PP_1318 0.59 +1.6 1,505,603 + PP_1318 0.59 +1.0 1,505,604 - PP_1318 0.59 -0.0 1,505,606 + PP_1318 0.59 -0.0 1,505,607 - PP_1318 0.59 +1.0 1,505,607 - PP_1318 0.59 +1.0 1,505,690 + PP_1318 0.66 -0.0 1,505,690 + PP_1318 0.66 -0.0 1,505,778 - PP_1318 0.73 -0.0 1,505,778 - PP_1318 0.73 +2.0
Or see this region's nucleotide sequence