Experiment: 72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 30% DO with 20% v/v oleyl alcohol overlay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0833 and PP_0834 are separated by 42 nucleotides PP_0834 and PP_0835 are separated by 63 nucleotides PP_0835 and PP_0836 are separated by 10 nucleotides
PP_0833: PP_0833 - queuine tRNA-ribosyltransferase, at 970,152 to 971,267
_0833
PP_0834: PP_0834 - Sec translocation complex subunit YajC, at 971,310 to 971,645
_0834
PP_0835: PP_0835 - Sec Translocation Complex SecD subunit, at 971,709 to 973,571
_0835
PP_0836: PP_0836 - Sec translocation complex subunit SecF, at 973,582 to 974,490
_0836
Position (kb) 971
972
973
974 Strain fitness (log2 ratio) -1
0
1 at 970.716 kb on + strand, within PP_0833 at 970.955 kb on + strand, within PP_0833 at 970.985 kb on + strand, within PP_0833 at 971.060 kb on + strand, within PP_0833 at 971.085 kb on + strand, within PP_0833 at 971.135 kb on + strand, within PP_0833 at 971.201 kb on + strand at 971.298 kb on + strand at 971.298 kb on + strand at 971.298 kb on + strand at 971.418 kb on + strand, within PP_0834 at 971.418 kb on + strand, within PP_0834 at 971.418 kb on + strand, within PP_0834 at 971.639 kb on + strand at 974.488 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 30% DO with 20% v/v oleyl alcohol overlay remove 970,716 + PP_0833 0.51 +1.0 970,955 + PP_0833 0.72 +1.0 970,985 + PP_0833 0.75 +1.0 971,060 + PP_0833 0.81 +1.0 971,085 + PP_0833 0.84 +1.0 971,135 + PP_0833 0.88 -0.0 971,201 + +1.0 971,298 + +0.1 971,298 + -0.8 971,298 + +1.0 971,418 + PP_0834 0.32 +1.0 971,418 + PP_0834 0.32 +1.0 971,418 + PP_0834 0.32 -0.0 971,639 + -1.3 974,488 + -0.0
Or see this region's nucleotide sequence