Experiment: 72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 30% DO with 20% v/v oleyl alcohol overlay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0381 and PP_0382 are separated by 140 nucleotides PP_0382 and PP_0383 are separated by 14 nucleotides
PP_0381: PP_0381 - Coenzyme PQQ synthesis protein F, at 460,164 to 462,464
_0381
PP_0382: PP_0382 - 5-aminopentanamidase, at 462,605 to 463,399
_0382
PP_0383: PP_0383 - Lysine 2-monooxygenase, at 463,414 to 465,096
_0383
Position (kb)
462
463
464 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 461.611 kb on + strand, within PP_0381 at 461.612 kb on - strand, within PP_0381 at 461.672 kb on + strand, within PP_0381 at 461.696 kb on - strand, within PP_0381 at 461.908 kb on + strand, within PP_0381 at 461.908 kb on + strand, within PP_0381 at 461.972 kb on - strand, within PP_0381 at 462.253 kb on + strand at 462.593 kb on + strand at 462.593 kb on + strand at 462.594 kb on - strand at 462.613 kb on + strand at 462.614 kb on - strand at 462.614 kb on - strand at 462.802 kb on - strand, within PP_0382 at 462.802 kb on - strand, within PP_0382 at 462.810 kb on + strand, within PP_0382 at 462.810 kb on + strand, within PP_0382 at 462.811 kb on - strand, within PP_0382 at 462.812 kb on + strand, within PP_0382 at 462.812 kb on + strand, within PP_0382 at 462.812 kb on + strand, within PP_0382 at 462.813 kb on - strand, within PP_0382 at 462.813 kb on - strand, within PP_0382 at 462.813 kb on - strand, within PP_0382 at 462.813 kb on - strand, within PP_0382 at 462.813 kb on - strand, within PP_0382 at 462.813 kb on - strand, within PP_0382 at 462.819 kb on + strand, within PP_0382 at 462.819 kb on + strand, within PP_0382 at 462.819 kb on + strand, within PP_0382 at 462.820 kb on - strand, within PP_0382 at 462.820 kb on - strand, within PP_0382 at 462.865 kb on + strand, within PP_0382 at 462.961 kb on - strand, within PP_0382 at 462.961 kb on - strand, within PP_0382 at 463.120 kb on - strand, within PP_0382 at 463.155 kb on + strand, within PP_0382 at 463.156 kb on - strand, within PP_0382 at 463.156 kb on - strand, within PP_0382 at 463.383 kb on + strand at 463.383 kb on + strand at 463.384 kb on - strand at 463.384 kb on - strand at 463.384 kb on - strand at 463.634 kb on + strand, within PP_0383 at 463.634 kb on + strand, within PP_0383 at 463.635 kb on - strand, within PP_0383 at 463.635 kb on - strand, within PP_0383 at 463.635 kb on - strand, within PP_0383 at 463.694 kb on + strand, within PP_0383 at 463.763 kb on + strand, within PP_0383 at 463.795 kb on + strand, within PP_0383 at 463.899 kb on - strand, within PP_0383 at 463.899 kb on - strand, within PP_0383 at 463.899 kb on - strand, within PP_0383 at 463.899 kb on - strand, within PP_0383 at 464.026 kb on - strand, within PP_0383 at 464.026 kb on - strand, within PP_0383 at 464.117 kb on - strand, within PP_0383 at 464.126 kb on + strand, within PP_0383 at 464.126 kb on + strand, within PP_0383 at 464.127 kb on - strand, within PP_0383
Per-strain Table
Position Strand Gene LocusTag Fraction 72hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 30% DO with 20% v/v oleyl alcohol overlay remove 461,611 + PP_0381 0.63 +0.4 461,612 - PP_0381 0.63 +0.0 461,672 + PP_0381 0.66 -0.3 461,696 - PP_0381 0.67 +0.0 461,908 + PP_0381 0.76 +0.3 461,908 + PP_0381 0.76 +0.0 461,972 - PP_0381 0.79 -1.0 462,253 + -1.4 462,593 + -0.9 462,593 + +0.4 462,594 - +0.3 462,613 + -0.2 462,614 - -0.6 462,614 - +0.3 462,802 - PP_0382 0.25 -0.1 462,802 - PP_0382 0.25 +1.0 462,810 + PP_0382 0.26 +0.5 462,810 + PP_0382 0.26 -0.1 462,811 - PP_0382 0.26 -0.4 462,812 + PP_0382 0.26 -0.7 462,812 + PP_0382 0.26 +0.0 462,812 + PP_0382 0.26 -1.4 462,813 - PP_0382 0.26 -0.4 462,813 - PP_0382 0.26 +0.3 462,813 - PP_0382 0.26 +0.7 462,813 - PP_0382 0.26 -0.8 462,813 - PP_0382 0.26 +0.0 462,813 - PP_0382 0.26 +0.4 462,819 + PP_0382 0.27 -0.6 462,819 + PP_0382 0.27 -1.0 462,819 + PP_0382 0.27 -0.1 462,820 - PP_0382 0.27 -1.3 462,820 - PP_0382 0.27 +1.4 462,865 + PP_0382 0.33 -1.0 462,961 - PP_0382 0.45 +0.6 462,961 - PP_0382 0.45 -0.4 463,120 - PP_0382 0.65 +0.7 463,155 + PP_0382 0.69 +0.1 463,156 - PP_0382 0.69 -1.0 463,156 - PP_0382 0.69 +0.3 463,383 + -0.5 463,383 + -0.1 463,384 - +0.4 463,384 - +0.0 463,384 - +0.1 463,634 + PP_0383 0.13 -2.1 463,634 + PP_0383 0.13 +1.4 463,635 - PP_0383 0.13 -0.8 463,635 - PP_0383 0.13 -0.4 463,635 - PP_0383 0.13 -0.6 463,694 + PP_0383 0.17 +0.0 463,763 + PP_0383 0.21 -0.6 463,795 + PP_0383 0.23 -0.2 463,899 - PP_0383 0.29 -1.0 463,899 - PP_0383 0.29 +1.0 463,899 - PP_0383 0.29 +0.8 463,899 - PP_0383 0.29 +0.0 464,026 - PP_0383 0.36 -0.2 464,026 - PP_0383 0.36 +0.7 464,117 - PP_0383 0.42 +0.5 464,126 + PP_0383 0.42 -1.1 464,126 + PP_0383 0.42 -0.4 464,127 - PP_0383 0.42 +2.6
Or see this region's nucleotide sequence