Experiment: LB Anaerobic with Ceftriaxone hemi(heptahydrate) 0.00000015625 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt alkA and yegD are separated by 133 nucleotides yegD and yegI are separated by 12 nucleotides
b2068: alkA - 3-methyl-adenine DNA glycosylase II (NCBI), at 2,144,716 to 2,145,564
alkA
b2069: yegD - putative heat shock protein (VIMSS), at 2,145,698 to 2,147,050
yegD
b2070: yegI - hypothetical protein (NCBI), at 2,147,063 to 2,149,009
yegI
Position (kb)
2145
2146
2147
2148 Strain fitness (log2 ratio)
-1
0
1
2 at 2144.721 kb on - strand at 2144.721 kb on - strand at 2144.753 kb on + strand at 2144.877 kb on + strand, within alkA at 2144.963 kb on - strand, within alkA at 2145.185 kb on + strand, within alkA at 2145.267 kb on + strand, within alkA at 2145.336 kb on + strand, within alkA at 2145.336 kb on + strand, within alkA at 2145.398 kb on - strand, within alkA at 2145.398 kb on - strand, within alkA at 2145.398 kb on - strand, within alkA at 2145.398 kb on - strand, within alkA at 2145.452 kb on - strand, within alkA at 2145.480 kb on + strand at 2145.540 kb on - strand at 2145.557 kb on + strand at 2145.565 kb on - strand at 2145.721 kb on - strand at 2145.726 kb on - strand at 2145.726 kb on - strand at 2145.794 kb on + strand at 2145.794 kb on + strand at 2145.911 kb on + strand, within yegD at 2145.936 kb on + strand, within yegD at 2146.210 kb on + strand, within yegD at 2146.262 kb on + strand, within yegD at 2146.348 kb on - strand, within yegD at 2146.351 kb on + strand, within yegD at 2146.351 kb on + strand, within yegD at 2146.359 kb on - strand, within yegD at 2146.361 kb on - strand, within yegD at 2146.361 kb on - strand, within yegD at 2146.368 kb on + strand, within yegD at 2146.368 kb on + strand, within yegD at 2146.538 kb on - strand, within yegD at 2146.565 kb on + strand, within yegD at 2146.583 kb on - strand, within yegD at 2146.615 kb on - strand, within yegD at 2146.661 kb on - strand, within yegD at 2146.716 kb on + strand, within yegD at 2146.724 kb on - strand, within yegD at 2146.724 kb on - strand, within yegD at 2146.724 kb on - strand, within yegD at 2146.724 kb on - strand, within yegD at 2146.749 kb on - strand, within yegD at 2146.749 kb on - strand, within yegD at 2146.902 kb on + strand, within yegD at 2147.147 kb on - strand at 2147.151 kb on + strand at 2147.181 kb on - strand at 2147.334 kb on - strand, within yegI at 2147.667 kb on + strand, within yegI at 2147.724 kb on + strand, within yegI at 2147.822 kb on - strand, within yegI at 2147.848 kb on + strand, within yegI at 2147.848 kb on + strand, within yegI at 2147.922 kb on - strand, within yegI
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Ceftriaxone hemi(heptahydrate) 0.00000015625 mM remove 2,144,721 - -0.2 2,144,721 - -0.6 2,144,753 + +0.5 2,144,877 + alkA b2068 0.19 -0.1 2,144,963 - alkA b2068 0.29 -0.9 2,145,185 + alkA b2068 0.55 -1.2 2,145,267 + alkA b2068 0.65 -0.8 2,145,336 + alkA b2068 0.73 +0.6 2,145,336 + alkA b2068 0.73 +0.8 2,145,398 - alkA b2068 0.80 +0.1 2,145,398 - alkA b2068 0.80 -0.4 2,145,398 - alkA b2068 0.80 -0.1 2,145,398 - alkA b2068 0.80 +1.2 2,145,452 - alkA b2068 0.87 -0.5 2,145,480 + -0.0 2,145,540 - -0.2 2,145,557 + +1.2 2,145,565 - +0.1 2,145,721 - -0.6 2,145,726 - -0.2 2,145,726 - +2.0 2,145,794 + +0.6 2,145,794 + +0.7 2,145,911 + yegD b2069 0.16 +0.4 2,145,936 + yegD b2069 0.18 +0.4 2,146,210 + yegD b2069 0.38 -0.1 2,146,262 + yegD b2069 0.42 +1.7 2,146,348 - yegD b2069 0.48 -1.4 2,146,351 + yegD b2069 0.48 +0.1 2,146,351 + yegD b2069 0.48 +0.4 2,146,359 - yegD b2069 0.49 +0.5 2,146,361 - yegD b2069 0.49 +1.2 2,146,361 - yegD b2069 0.49 +0.7 2,146,368 + yegD b2069 0.50 +0.3 2,146,368 + yegD b2069 0.50 +0.4 2,146,538 - yegD b2069 0.62 +1.4 2,146,565 + yegD b2069 0.64 -0.1 2,146,583 - yegD b2069 0.65 +0.3 2,146,615 - yegD b2069 0.68 -0.9 2,146,661 - yegD b2069 0.71 +0.4 2,146,716 + yegD b2069 0.75 +0.7 2,146,724 - yegD b2069 0.76 +0.5 2,146,724 - yegD b2069 0.76 +0.0 2,146,724 - yegD b2069 0.76 +0.3 2,146,724 - yegD b2069 0.76 +1.4 2,146,749 - yegD b2069 0.78 +0.4 2,146,749 - yegD b2069 0.78 +0.4 2,146,902 + yegD b2069 0.89 +0.4 2,147,147 - -1.3 2,147,151 + +0.2 2,147,181 - -0.1 2,147,334 - yegI b2070 0.14 -0.4 2,147,667 + yegI b2070 0.31 +0.5 2,147,724 + yegI b2070 0.34 -0.4 2,147,822 - yegI b2070 0.39 -0.7 2,147,848 + yegI b2070 0.40 -0.6 2,147,848 + yegI b2070 0.40 +1.3 2,147,922 - yegI b2070 0.44 -0.3
Or see this region's nucleotide sequence