Strain Fitness in Escherichia coli BW25113 around b2069

Experiment: LB Anaerobic with Ceftriaxone hemi(heptahydrate) 0.00000015625 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntalkA and yegD are separated by 133 nucleotidesyegD and yegI are separated by 12 nucleotides b2068: alkA - 3-methyl-adenine DNA glycosylase II (NCBI), at 2,144,716 to 2,145,564 alkA b2069: yegD - putative heat shock protein (VIMSS), at 2,145,698 to 2,147,050 yegD b2070: yegI - hypothetical protein (NCBI), at 2,147,063 to 2,149,009 yegI Position (kb) 2145 2146 2147 2148Strain fitness (log2 ratio) -1 0 1 2at 2144.721 kb on - strandat 2144.721 kb on - strandat 2144.753 kb on + strandat 2144.877 kb on + strand, within alkAat 2144.963 kb on - strand, within alkAat 2145.185 kb on + strand, within alkAat 2145.267 kb on + strand, within alkAat 2145.336 kb on + strand, within alkAat 2145.336 kb on + strand, within alkAat 2145.398 kb on - strand, within alkAat 2145.398 kb on - strand, within alkAat 2145.398 kb on - strand, within alkAat 2145.398 kb on - strand, within alkAat 2145.452 kb on - strand, within alkAat 2145.480 kb on + strandat 2145.540 kb on - strandat 2145.557 kb on + strandat 2145.565 kb on - strandat 2145.721 kb on - strandat 2145.726 kb on - strandat 2145.726 kb on - strandat 2145.794 kb on + strandat 2145.794 kb on + strandat 2145.911 kb on + strand, within yegDat 2145.936 kb on + strand, within yegDat 2146.210 kb on + strand, within yegDat 2146.262 kb on + strand, within yegDat 2146.348 kb on - strand, within yegDat 2146.351 kb on + strand, within yegDat 2146.351 kb on + strand, within yegDat 2146.359 kb on - strand, within yegDat 2146.361 kb on - strand, within yegDat 2146.361 kb on - strand, within yegDat 2146.368 kb on + strand, within yegDat 2146.368 kb on + strand, within yegDat 2146.538 kb on - strand, within yegDat 2146.565 kb on + strand, within yegDat 2146.583 kb on - strand, within yegDat 2146.615 kb on - strand, within yegDat 2146.661 kb on - strand, within yegDat 2146.716 kb on + strand, within yegDat 2146.724 kb on - strand, within yegDat 2146.724 kb on - strand, within yegDat 2146.724 kb on - strand, within yegDat 2146.724 kb on - strand, within yegDat 2146.749 kb on - strand, within yegDat 2146.749 kb on - strand, within yegDat 2146.902 kb on + strand, within yegDat 2147.147 kb on - strandat 2147.151 kb on + strandat 2147.181 kb on - strandat 2147.334 kb on - strand, within yegIat 2147.667 kb on + strand, within yegIat 2147.724 kb on + strand, within yegIat 2147.822 kb on - strand, within yegIat 2147.848 kb on + strand, within yegIat 2147.848 kb on + strand, within yegIat 2147.922 kb on - strand, within yegI

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Anaerobic with Ceftriaxone hemi(heptahydrate) 0.00000015625 mM
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2,144,721 - -0.2
2,144,721 - -0.6
2,144,753 + +0.5
2,144,877 + alkA b2068 0.19 -0.1
2,144,963 - alkA b2068 0.29 -0.9
2,145,185 + alkA b2068 0.55 -1.2
2,145,267 + alkA b2068 0.65 -0.8
2,145,336 + alkA b2068 0.73 +0.6
2,145,336 + alkA b2068 0.73 +0.8
2,145,398 - alkA b2068 0.80 +0.1
2,145,398 - alkA b2068 0.80 -0.4
2,145,398 - alkA b2068 0.80 -0.1
2,145,398 - alkA b2068 0.80 +1.2
2,145,452 - alkA b2068 0.87 -0.5
2,145,480 + -0.0
2,145,540 - -0.2
2,145,557 + +1.2
2,145,565 - +0.1
2,145,721 - -0.6
2,145,726 - -0.2
2,145,726 - +2.0
2,145,794 + +0.6
2,145,794 + +0.7
2,145,911 + yegD b2069 0.16 +0.4
2,145,936 + yegD b2069 0.18 +0.4
2,146,210 + yegD b2069 0.38 -0.1
2,146,262 + yegD b2069 0.42 +1.7
2,146,348 - yegD b2069 0.48 -1.4
2,146,351 + yegD b2069 0.48 +0.1
2,146,351 + yegD b2069 0.48 +0.4
2,146,359 - yegD b2069 0.49 +0.5
2,146,361 - yegD b2069 0.49 +1.2
2,146,361 - yegD b2069 0.49 +0.7
2,146,368 + yegD b2069 0.50 +0.3
2,146,368 + yegD b2069 0.50 +0.4
2,146,538 - yegD b2069 0.62 +1.4
2,146,565 + yegD b2069 0.64 -0.1
2,146,583 - yegD b2069 0.65 +0.3
2,146,615 - yegD b2069 0.68 -0.9
2,146,661 - yegD b2069 0.71 +0.4
2,146,716 + yegD b2069 0.75 +0.7
2,146,724 - yegD b2069 0.76 +0.5
2,146,724 - yegD b2069 0.76 +0.0
2,146,724 - yegD b2069 0.76 +0.3
2,146,724 - yegD b2069 0.76 +1.4
2,146,749 - yegD b2069 0.78 +0.4
2,146,749 - yegD b2069 0.78 +0.4
2,146,902 + yegD b2069 0.89 +0.4
2,147,147 - -1.3
2,147,151 + +0.2
2,147,181 - -0.1
2,147,334 - yegI b2070 0.14 -0.4
2,147,667 + yegI b2070 0.31 +0.5
2,147,724 + yegI b2070 0.34 -0.4
2,147,822 - yegI b2070 0.39 -0.7
2,147,848 + yegI b2070 0.40 -0.6
2,147,848 + yegI b2070 0.40 +1.3
2,147,922 - yegI b2070 0.44 -0.3

Or see this region's nucleotide sequence