Strain Fitness in Escherichia coli BW25113 around b0949

Experiment: LB Anaerobic with Ceftriaxone hemi(heptahydrate) 0.000000625 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntycbY and uup are separated by 11 nucleotidesuup and pqiA are separated by 129 nucleotides b0948: ycbY - predicted methyltransferase (NCBI), at 1,007,067 to 1,009,175 ycbY b0949: uup - fused predicted transporter subunits of ABC superfamily: ATP-binding components (NCBI), at 1,009,187 to 1,011,094 uup b0950: pqiA - paraquat-inducible membrane protein A (NCBI), at 1,011,224 to 1,012,477 pqiA Position (kb) 1009 1010 1011 1012Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 1008.248 kb on - strand, within ycbYat 1008.248 kb on - strand, within ycbYat 1008.322 kb on - strand, within ycbYat 1008.322 kb on - strand, within ycbYat 1008.446 kb on - strand, within ycbYat 1008.573 kb on - strand, within ycbYat 1008.573 kb on - strand, within ycbYat 1008.588 kb on + strand, within ycbYat 1008.588 kb on + strand, within ycbYat 1008.593 kb on + strand, within ycbYat 1008.622 kb on + strand, within ycbYat 1008.679 kb on - strand, within ycbYat 1008.735 kb on + strand, within ycbYat 1008.749 kb on + strand, within ycbYat 1008.749 kb on + strand, within ycbYat 1008.757 kb on - strand, within ycbYat 1008.757 kb on - strand, within ycbYat 1008.778 kb on - strand, within ycbYat 1008.849 kb on - strand, within ycbYat 1009.006 kb on + strandat 1009.048 kb on + strandat 1009.048 kb on + strandat 1009.159 kb on - strandat 1009.159 kb on - strandat 1009.178 kb on - strandat 1009.178 kb on - strandat 1009.256 kb on - strandat 1009.256 kb on - strandat 1009.560 kb on - strand, within uupat 1009.560 kb on - strand, within uupat 1009.581 kb on - strand, within uupat 1009.581 kb on - strand, within uupat 1009.648 kb on + strand, within uupat 1009.699 kb on + strand, within uupat 1009.699 kb on + strand, within uupat 1009.707 kb on - strand, within uupat 1009.772 kb on + strand, within uupat 1009.814 kb on + strand, within uupat 1009.814 kb on + strand, within uupat 1009.839 kb on + strand, within uupat 1009.839 kb on + strand, within uupat 1009.904 kb on - strand, within uupat 1009.996 kb on - strand, within uupat 1010.004 kb on + strand, within uupat 1010.156 kb on - strand, within uupat 1010.325 kb on - strand, within uupat 1010.439 kb on + strand, within uupat 1010.589 kb on - strand, within uupat 1010.589 kb on - strand, within uupat 1010.662 kb on - strand, within uupat 1010.752 kb on - strand, within uupat 1010.782 kb on + strand, within uupat 1010.882 kb on - strand, within uupat 1010.882 kb on - strand, within uupat 1011.118 kb on + strandat 1011.607 kb on - strand, within pqiAat 1011.620 kb on - strand, within pqiAat 1011.748 kb on + strand, within pqiAat 1011.794 kb on + strand, within pqiAat 1011.876 kb on + strand, within pqiAat 1011.956 kb on - strand, within pqiAat 1011.982 kb on + strand, within pqiAat 1012.008 kb on - strand, within pqiAat 1012.008 kb on - strand, within pqiAat 1012.011 kb on + strand, within pqiAat 1012.067 kb on + strand, within pqiAat 1012.067 kb on + strand, within pqiA

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Anaerobic with Ceftriaxone hemi(heptahydrate) 0.000000625 mM
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1,008,248 - ycbY b0948 0.56 +0.1
1,008,248 - ycbY b0948 0.56 -0.2
1,008,322 - ycbY b0948 0.60 -3.2
1,008,322 - ycbY b0948 0.60 -1.5
1,008,446 - ycbY b0948 0.65 -0.8
1,008,573 - ycbY b0948 0.71 +1.8
1,008,573 - ycbY b0948 0.71 +0.3
1,008,588 + ycbY b0948 0.72 -0.2
1,008,588 + ycbY b0948 0.72 +0.1
1,008,593 + ycbY b0948 0.72 -0.3
1,008,622 + ycbY b0948 0.74 -1.7
1,008,679 - ycbY b0948 0.76 -0.4
1,008,735 + ycbY b0948 0.79 +0.5
1,008,749 + ycbY b0948 0.80 -0.5
1,008,749 + ycbY b0948 0.80 -0.8
1,008,757 - ycbY b0948 0.80 +0.3
1,008,757 - ycbY b0948 0.80 -1.1
1,008,778 - ycbY b0948 0.81 -1.1
1,008,849 - ycbY b0948 0.84 +1.1
1,009,006 + -0.1
1,009,048 + +1.3
1,009,048 + -0.2
1,009,159 - -1.5
1,009,159 - -0.3
1,009,178 - -1.3
1,009,178 - +1.5
1,009,256 - -2.4
1,009,256 - +0.8
1,009,560 - uup b0949 0.20 -0.6
1,009,560 - uup b0949 0.20 +0.8
1,009,581 - uup b0949 0.21 +0.2
1,009,581 - uup b0949 0.21 -0.6
1,009,648 + uup b0949 0.24 -0.0
1,009,699 + uup b0949 0.27 -0.2
1,009,699 + uup b0949 0.27 -0.2
1,009,707 - uup b0949 0.27 +0.5
1,009,772 + uup b0949 0.31 -0.1
1,009,814 + uup b0949 0.33 +1.8
1,009,814 + uup b0949 0.33 +1.4
1,009,839 + uup b0949 0.34 +1.0
1,009,839 + uup b0949 0.34 -0.2
1,009,904 - uup b0949 0.38 -0.6
1,009,996 - uup b0949 0.42 +1.2
1,010,004 + uup b0949 0.43 +0.3
1,010,156 - uup b0949 0.51 +1.5
1,010,325 - uup b0949 0.60 -1.8
1,010,439 + uup b0949 0.66 +0.5
1,010,589 - uup b0949 0.73 -1.0
1,010,589 - uup b0949 0.73 +0.6
1,010,662 - uup b0949 0.77 -0.7
1,010,752 - uup b0949 0.82 +2.8
1,010,782 + uup b0949 0.84 +0.2
1,010,882 - uup b0949 0.89 +1.2
1,010,882 - uup b0949 0.89 -0.1
1,011,118 + -0.2
1,011,607 - pqiA b0950 0.31 -0.6
1,011,620 - pqiA b0950 0.32 -0.1
1,011,748 + pqiA b0950 0.42 -0.8
1,011,794 + pqiA b0950 0.45 -0.2
1,011,876 + pqiA b0950 0.52 -1.8
1,011,956 - pqiA b0950 0.58 -0.4
1,011,982 + pqiA b0950 0.60 -0.7
1,012,008 - pqiA b0950 0.63 -0.0
1,012,008 - pqiA b0950 0.63 +1.2
1,012,011 + pqiA b0950 0.63 -1.0
1,012,067 + pqiA b0950 0.67 -0.1
1,012,067 + pqiA b0950 0.67 -0.4

Or see this region's nucleotide sequence