Experiment: LB Anaerobic with Ceftriaxone hemi(heptahydrate) 0.000000625 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ykgT and yagQ overlap by 22 nucleotides yagQ and yagR are separated by 9 nucleotides yagR and yagS overlap by 4 nucleotides yagS and yagT overlap by 4 nucleotides
b4695: ykgT - no description, at 296,938 to 297,015
ykgT
b0283: yagQ - hypothetical protein (NCBI), at 296,994 to 297,950
yagQ
b0284: yagR - predicted oxidoreductase with molybdenum-binding domain (NCBI), at 297,960 to 300,158
yagR
b0285: yagS - predicted oxidoreductase with FAD-binding domain (NCBI), at 300,155 to 301,111
yagS
b0286: yagT - predicted xanthine dehydrogenase, 2Fe-2S subunit (NCBI), at 301,108 to 301,797
yagT
Position (kb) 297
298
299
300
301 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 296.991 kb on + strand, within ykgT at 296.993 kb on - strand, within ykgT at 296.993 kb on - strand, within ykgT at 297.046 kb on - strand at 297.064 kb on + strand at 297.064 kb on + strand at 297.068 kb on + strand at 297.068 kb on + strand at 297.082 kb on - strand at 297.111 kb on - strand, within yagQ at 297.111 kb on - strand, within yagQ at 297.144 kb on - strand, within yagQ at 297.144 kb on - strand, within yagQ at 297.185 kb on + strand, within yagQ at 297.185 kb on + strand, within yagQ at 297.193 kb on - strand, within yagQ at 297.226 kb on - strand, within yagQ at 297.226 kb on - strand, within yagQ at 297.316 kb on - strand, within yagQ at 297.316 kb on - strand, within yagQ at 297.432 kb on - strand, within yagQ at 297.455 kb on - strand, within yagQ at 297.455 kb on - strand, within yagQ at 297.513 kb on + strand, within yagQ at 297.581 kb on + strand, within yagQ at 297.950 kb on - strand at 298.066 kb on + strand at 298.138 kb on - strand at 298.138 kb on - strand at 298.187 kb on - strand, within yagR at 298.267 kb on - strand, within yagR at 298.267 kb on - strand, within yagR at 298.449 kb on + strand, within yagR at 298.449 kb on + strand, within yagR at 298.549 kb on - strand, within yagR at 298.581 kb on + strand, within yagR at 298.632 kb on + strand, within yagR at 298.728 kb on + strand, within yagR at 298.738 kb on + strand, within yagR at 298.770 kb on + strand, within yagR at 298.903 kb on + strand, within yagR at 298.906 kb on - strand, within yagR at 298.908 kb on + strand, within yagR at 298.908 kb on + strand, within yagR at 298.908 kb on + strand, within yagR at 299.169 kb on + strand, within yagR at 299.206 kb on - strand, within yagR at 299.206 kb on - strand, within yagR at 299.218 kb on + strand, within yagR at 299.218 kb on + strand, within yagR at 299.341 kb on - strand, within yagR at 299.341 kb on - strand, within yagR at 299.413 kb on + strand, within yagR at 299.543 kb on + strand, within yagR at 299.952 kb on + strand at 299.952 kb on + strand at 300.038 kb on + strand at 300.109 kb on + strand at 300.109 kb on + strand at 300.176 kb on + strand at 300.181 kb on + strand at 300.181 kb on + strand at 300.196 kb on - strand at 300.268 kb on - strand, within yagS at 300.268 kb on - strand, within yagS at 300.325 kb on + strand, within yagS at 300.385 kb on + strand, within yagS at 300.483 kb on + strand, within yagS at 300.486 kb on - strand, within yagS at 300.486 kb on - strand, within yagS at 300.544 kb on + strand, within yagS at 300.552 kb on - strand, within yagS at 300.581 kb on + strand, within yagS at 300.719 kb on + strand, within yagS at 300.798 kb on + strand, within yagS at 300.829 kb on - strand, within yagS at 300.887 kb on - strand, within yagS at 300.916 kb on - strand, within yagS at 301.099 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Ceftriaxone hemi(heptahydrate) 0.000000625 mM remove 296,991 + ykgT b4695 0.68 -0.9 296,993 - ykgT b4695 0.71 -1.5 296,993 - ykgT b4695 0.71 -0.5 297,046 - -1.1 297,064 + -0.5 297,064 + -0.4 297,068 + -1.6 297,068 + +1.2 297,082 - -0.9 297,111 - yagQ b0283 0.12 +0.6 297,111 - yagQ b0283 0.12 +0.3 297,144 - yagQ b0283 0.16 +0.1 297,144 - yagQ b0283 0.16 -0.8 297,185 + yagQ b0283 0.20 +0.1 297,185 + yagQ b0283 0.20 +0.4 297,193 - yagQ b0283 0.21 -0.2 297,226 - yagQ b0283 0.24 +2.1 297,226 - yagQ b0283 0.24 +0.5 297,316 - yagQ b0283 0.34 +0.2 297,316 - yagQ b0283 0.34 -0.2 297,432 - yagQ b0283 0.46 -1.8 297,455 - yagQ b0283 0.48 +0.5 297,455 - yagQ b0283 0.48 +0.6 297,513 + yagQ b0283 0.54 +0.2 297,581 + yagQ b0283 0.61 +0.1 297,950 - +1.1 298,066 + -0.5 298,138 - +0.8 298,138 - -0.9 298,187 - yagR b0284 0.10 -0.5 298,267 - yagR b0284 0.14 -1.1 298,267 - yagR b0284 0.14 -1.0 298,449 + yagR b0284 0.22 -0.7 298,449 + yagR b0284 0.22 -1.1 298,549 - yagR b0284 0.27 -0.9 298,581 + yagR b0284 0.28 +0.5 298,632 + yagR b0284 0.31 -0.1 298,728 + yagR b0284 0.35 -1.3 298,738 + yagR b0284 0.35 +0.1 298,770 + yagR b0284 0.37 +0.1 298,903 + yagR b0284 0.43 +0.2 298,906 - yagR b0284 0.43 +0.2 298,908 + yagR b0284 0.43 -1.5 298,908 + yagR b0284 0.43 -2.5 298,908 + yagR b0284 0.43 +0.5 299,169 + yagR b0284 0.55 -1.9 299,206 - yagR b0284 0.57 -2.9 299,206 - yagR b0284 0.57 +1.5 299,218 + yagR b0284 0.57 -0.3 299,218 + yagR b0284 0.57 -0.6 299,341 - yagR b0284 0.63 -1.1 299,341 - yagR b0284 0.63 -0.3 299,413 + yagR b0284 0.66 +0.3 299,543 + yagR b0284 0.72 +0.3 299,952 + +0.4 299,952 + -0.6 300,038 + -0.2 300,109 + -0.3 300,109 + +0.9 300,176 + -0.0 300,181 + -1.2 300,181 + +0.1 300,196 - +0.3 300,268 - yagS b0285 0.12 -0.1 300,268 - yagS b0285 0.12 -2.0 300,325 + yagS b0285 0.18 -0.3 300,385 + yagS b0285 0.24 -0.0 300,483 + yagS b0285 0.34 +0.4 300,486 - yagS b0285 0.35 +0.5 300,486 - yagS b0285 0.35 -0.0 300,544 + yagS b0285 0.41 -0.9 300,552 - yagS b0285 0.41 +0.2 300,581 + yagS b0285 0.45 +0.2 300,719 + yagS b0285 0.59 +0.2 300,798 + yagS b0285 0.67 -0.0 300,829 - yagS b0285 0.70 +0.2 300,887 - yagS b0285 0.76 -1.8 300,916 - yagS b0285 0.80 -0.1 301,099 - -0.4
Or see this region's nucleotide sequence