Experiment: 48hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 30% DO with 20% v/v dodecane overlay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_5036 and PP_5037 are separated by 70 nucleotides PP_5037 and PP_5038 overlap by 4 nucleotides PP_5038 and PP_5039 are separated by 227 nucleotides PP_5039 and PP_5040 are separated by 3 nucleotides
PP_5036: PP_5036 - probable formiminoglutamate deiminase, at 5,739,071 to 5,740,435
_5036
PP_5037: PP_5037 - lipocalin family lipoprotein, at 5,740,506 to 5,741,078
_5037
PP_5038: PP_5038 - conserved lipoprotein of unknown function, at 5,741,075 to 5,741,329
_5038
PP_5039: PP_5039 - conserved protein of unknown function, at 5,741,557 to 5,742,153
_5039
PP_5040: PP_5040 - fructose-1,6-bisphosphatase, class 1, at 5,742,157 to 5,743,167
_5040
Position (kb) 5741
5742 Strain fitness (log2 ratio) -2
-1
0
1 at 5740.079 kb on + strand, within PP_5036 at 5740.154 kb on + strand, within PP_5036 at 5740.154 kb on + strand, within PP_5036 at 5740.155 kb on - strand, within PP_5036 at 5740.259 kb on + strand, within PP_5036 at 5740.259 kb on + strand, within PP_5036 at 5740.519 kb on - strand at 5740.521 kb on + strand at 5740.645 kb on + strand, within PP_5037 at 5740.645 kb on + strand, within PP_5037 at 5740.645 kb on + strand, within PP_5037 at 5740.676 kb on + strand, within PP_5037 at 5740.677 kb on - strand, within PP_5037 at 5740.681 kb on + strand, within PP_5037 at 5740.681 kb on + strand, within PP_5037 at 5740.732 kb on - strand, within PP_5037 at 5740.732 kb on - strand, within PP_5037 at 5740.797 kb on - strand, within PP_5037 at 5740.861 kb on + strand, within PP_5037 at 5740.861 kb on + strand, within PP_5037 at 5740.861 kb on + strand, within PP_5037 at 5740.861 kb on + strand, within PP_5037 at 5740.885 kb on + strand, within PP_5037 at 5740.886 kb on - strand, within PP_5037 at 5740.945 kb on + strand, within PP_5037 at 5740.946 kb on - strand, within PP_5037 at 5741.065 kb on + strand at 5741.065 kb on + strand at 5741.065 kb on + strand at 5741.065 kb on + strand at 5741.065 kb on + strand at 5741.066 kb on - strand at 5741.098 kb on - strand at 5741.121 kb on + strand, within PP_5038 at 5741.122 kb on - strand, within PP_5038 at 5741.122 kb on - strand, within PP_5038 at 5741.233 kb on + strand, within PP_5038 at 5741.253 kb on + strand, within PP_5038 at 5741.253 kb on + strand, within PP_5038 at 5741.353 kb on + strand at 5741.353 kb on + strand at 5741.354 kb on - strand at 5741.354 kb on - strand at 5741.419 kb on + strand at 5741.420 kb on - strand at 5741.420 kb on - strand at 5741.420 kb on - strand at 5741.420 kb on - strand at 5741.427 kb on + strand at 5741.427 kb on + strand at 5741.490 kb on + strand at 5741.834 kb on + strand, within PP_5039 at 5741.915 kb on + strand, within PP_5039 at 5741.915 kb on + strand, within PP_5039 at 5742.050 kb on + strand, within PP_5039 at 5742.159 kb on - strand at 5742.159 kb on - strand at 5742.159 kb on - strand at 5742.159 kb on - strand at 5742.159 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 48hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 30% DO with 20% v/v dodecane overlay remove 5,740,079 + PP_5036 0.74 +0.5 5,740,154 + PP_5036 0.79 -0.0 5,740,154 + PP_5036 0.79 -0.4 5,740,155 - PP_5036 0.79 +0.5 5,740,259 + PP_5036 0.87 +0.5 5,740,259 + PP_5036 0.87 +1.1 5,740,519 - +0.0 5,740,521 + +0.5 5,740,645 + PP_5037 0.24 -0.1 5,740,645 + PP_5037 0.24 -0.5 5,740,645 + PP_5037 0.24 -0.8 5,740,676 + PP_5037 0.30 -0.1 5,740,677 - PP_5037 0.30 +0.2 5,740,681 + PP_5037 0.31 -1.1 5,740,681 + PP_5037 0.31 +0.2 5,740,732 - PP_5037 0.39 -0.2 5,740,732 - PP_5037 0.39 +0.3 5,740,797 - PP_5037 0.51 +1.5 5,740,861 + PP_5037 0.62 -0.6 5,740,861 + PP_5037 0.62 -0.8 5,740,861 + PP_5037 0.62 -0.5 5,740,861 + PP_5037 0.62 -0.2 5,740,885 + PP_5037 0.66 +0.7 5,740,886 - PP_5037 0.66 -1.5 5,740,945 + PP_5037 0.77 -1.3 5,740,946 - PP_5037 0.77 -0.8 5,741,065 + +0.4 5,741,065 + -0.5 5,741,065 + +0.1 5,741,065 + +0.1 5,741,065 + +0.3 5,741,066 - -0.6 5,741,098 - -0.5 5,741,121 + PP_5038 0.18 +0.4 5,741,122 - PP_5038 0.18 +0.5 5,741,122 - PP_5038 0.18 +0.9 5,741,233 + PP_5038 0.62 +0.4 5,741,253 + PP_5038 0.70 +0.5 5,741,253 + PP_5038 0.70 +0.1 5,741,353 + -0.4 5,741,353 + -1.0 5,741,354 - +0.6 5,741,354 - -0.5 5,741,419 + +0.1 5,741,420 - -0.5 5,741,420 - +0.1 5,741,420 - -0.1 5,741,420 - +0.3 5,741,427 + +0.3 5,741,427 + +0.9 5,741,490 + -0.2 5,741,834 + PP_5039 0.46 +0.5 5,741,915 + PP_5039 0.60 +1.5 5,741,915 + PP_5039 0.60 -0.1 5,742,050 + PP_5039 0.83 -0.7 5,742,159 - -2.4 5,742,159 - -0.8 5,742,159 - -0.5 5,742,159 - -1.5 5,742,159 - -2.1
Or see this region's nucleotide sequence