Strain Fitness in Pseudomonas putida KT2440 around PP_2256

Experiment: 48hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 30% DO with 20% v/v dodecane overlay

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	48hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 30% DO with 20% v/v dodecane overlay
2,571,670	+		PP_2255	0.41	-1.0
2,571,671	-		PP_2255	0.42	+0.2
2,571,754	+		PP_2255	0.48	-1.0
2,571,755	-		PP_2255	0.48	-0.8
2,571,803	-		PP_2255	0.52	-0.4
2,572,118	+		PP_2255	0.76	+0.6
2,572,118	+		PP_2255	0.76	-0.0
2,572,119	-		PP_2255	0.76	+0.5
2,572,198	+		PP_2255	0.82	+0.3
2,572,541	-				+2.2
2,572,541	-				-0.3
2,572,624	+				+0.3
2,572,660	+				+0.6
2,572,660	+				-0.8
2,572,661	-				-0.0
2,572,750	-		PP_2256	0.27	-1.0
2,572,851	+		PP_2256	0.59	-0.6
2,572,851	+		PP_2256	0.59	+0.7
2,572,851	+		PP_2256	0.59	-0.1
2,572,851	+		PP_2256	0.59	+0.3
2,572,852	-		PP_2256	0.60	-0.4
2,572,941	+		PP_2256	0.88	-0.4
2,573,015	-				+0.3
2,573,015	-				-0.4
2,573,338	+				+0.8
2,573,338	+				+0.6
2,573,338	+				+0.6
2,573,338	+				-0.4
2,573,339	-				-1.0
2,573,339	-				-0.7
2,573,371	+				-1.0
2,573,441	-		PP_2257	0.13	+0.1
2,573,441	-		PP_2257	0.13	+1.4
2,573,518	+		PP_2257	0.17	-1.0
2,573,519	-		PP_2257	0.17	-0.2
2,573,519	-		PP_2257	0.17	-1.0
2,573,519	-		PP_2257	0.17	-0.7
2,573,521	+		PP_2257	0.18	+0.4
2,573,521	+		PP_2257	0.18	-2.1
2,573,521	+		PP_2257	0.18	+0.6
2,573,521	+		PP_2257	0.18	+0.7
2,573,522	-		PP_2257	0.18	-1.0
2,573,522	-		PP_2257	0.18	-1.0
2,573,522	-		PP_2257	0.18	-0.5
2,573,536	+		PP_2257	0.19	+1.2
2,573,536	+		PP_2257	0.19	-0.7
2,573,536	+		PP_2257	0.19	-0.1
2,573,592	-		PP_2257	0.22	-0.7
2,573,599	-		PP_2257	0.23	-0.4
2,573,612	+		PP_2257	0.23	+0.5
2,573,896	+		PP_2257	0.42	+0.9
2,573,896	+		PP_2257	0.42	-0.3