Experiment: 48hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 30% DO with 20% v/v dodecane overlay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_1048 and PP_1049 overlap by 4 nucleotides PP_1049 and PP_1050 are separated by 3 nucleotides PP_1050 and PP_1051 overlap by 43 nucleotides PP_1051 and PP_1052 overlap by 4 nucleotides PP_1052 and PP_1053 overlap by 16 nucleotides
PP_1048: PP_1048 - putative protein secretion protein for export, at 1,199,146 to 1,200,348
_1048
PP_1049: PP_1049 - putative protein secretion protein for export, at 1,200,345 to 1,200,800
_1049
PP_1050: PP_1050 - Type II secretion pathway protein XcpU, at 1,200,804 to 1,201,235
_1050
PP_1051: PP_1051 - Type II secretion pathway protein XcpV, at 1,201,193 to 1,201,600
_1051
PP_1052: PP_1052 - Type II secretion pathway protein XcpW, at 1,201,597 to 1,202,187
_1052
PP_1053: PP_1053 - Type II secretion pathway protein XcpY, at 1,202,172 to 1,203,260
_1053
Position (kb) 1201
1202 Strain fitness (log2 ratio) -2
-1
0
1
2 at 1200.359 kb on + strand at 1200.360 kb on - strand at 1200.360 kb on - strand at 1200.360 kb on - strand at 1200.428 kb on + strand, within PP_1049 at 1200.428 kb on + strand, within PP_1049 at 1200.543 kb on + strand, within PP_1049 at 1200.543 kb on + strand, within PP_1049 at 1200.544 kb on - strand at 1200.544 kb on - strand, within PP_1049 at 1200.544 kb on - strand, within PP_1049 at 1200.544 kb on - strand, within PP_1049 at 1200.648 kb on + strand, within PP_1049 at 1200.649 kb on - strand, within PP_1049 at 1200.688 kb on - strand, within PP_1049 at 1200.688 kb on - strand, within PP_1049 at 1200.730 kb on - strand, within PP_1049 at 1200.798 kb on + strand at 1200.799 kb on - strand at 1201.157 kb on + strand, within PP_1050 at 1201.275 kb on + strand, within PP_1051 at 1201.320 kb on + strand, within PP_1051 at 1201.321 kb on - strand, within PP_1051 at 1201.418 kb on + strand, within PP_1051 at 1201.419 kb on - strand, within PP_1051 at 1201.574 kb on + strand at 1201.575 kb on - strand at 1201.575 kb on - strand at 1201.575 kb on - strand at 1201.575 kb on - strand at 1201.984 kb on + strand, within PP_1052 at 1202.149 kb on + strand at 1202.149 kb on + strand at 1202.149 kb on + strand at 1202.150 kb on - strand at 1202.150 kb on - strand at 1202.208 kb on - strand at 1202.560 kb on + strand, within PP_1053
Per-strain Table
Position Strand Gene LocusTag Fraction 48hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 30% DO with 20% v/v dodecane overlay remove 1,200,359 + -0.3 1,200,360 - -0.3 1,200,360 - -0.4 1,200,360 - +1.0 1,200,428 + PP_1049 0.18 -0.5 1,200,428 + PP_1049 0.18 +0.3 1,200,543 + PP_1049 0.43 -2.4 1,200,543 + PP_1049 0.43 +0.4 1,200,544 - -1.0 1,200,544 - PP_1049 0.44 +0.4 1,200,544 - PP_1049 0.44 -0.6 1,200,544 - PP_1049 0.44 +0.2 1,200,648 + PP_1049 0.66 -0.1 1,200,649 - PP_1049 0.67 +0.6 1,200,688 - PP_1049 0.75 +1.3 1,200,688 - PP_1049 0.75 -1.0 1,200,730 - PP_1049 0.84 +0.2 1,200,798 + +0.6 1,200,799 - -1.4 1,201,157 + PP_1050 0.82 -2.4 1,201,275 + PP_1051 0.20 -1.1 1,201,320 + PP_1051 0.31 +0.0 1,201,321 - PP_1051 0.31 -0.9 1,201,418 + PP_1051 0.55 -1.0 1,201,419 - PP_1051 0.55 -0.5 1,201,574 + -0.1 1,201,575 - -0.1 1,201,575 - +1.1 1,201,575 - -2.3 1,201,575 - +1.6 1,201,984 + PP_1052 0.65 +2.2 1,202,149 + -0.9 1,202,149 + -0.4 1,202,149 + -1.5 1,202,150 - +0.6 1,202,150 - +0.4 1,202,208 - +1.0 1,202,560 + PP_1053 0.36 -0.8
Or see this region's nucleotide sequence