Experiment: 48hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 30% DO with 20% v/v dodecane overlay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_1028 and PP_1029 are separated by 97 nucleotides PP_1029 and PP_1030 are separated by 47 nucleotides PP_1030 and PP_1031 are separated by 86 nucleotides
PP_1028: PP_1028 - Transcriptional regulator, LysR family, at 1,173,147 to 1,174,025
_1028
PP_1029: PP_1029 - conserved membrane protein of unknown function, at 1,174,123 to 1,174,878
_1029
PP_1030: PP_1030 - conserved protein of unknown function, at 1,174,926 to 1,175,483
_1030
PP_1031: PP_1031 - IMP dehydrogenase and single strand DNA binding factor, at 1,175,570 to 1,177,039
_1031
Position (kb) 1174
1175
1176 Strain fitness (log2 ratio) -1
0
1 at 1173.946 kb on + strand at 1173.946 kb on + strand at 1173.947 kb on - strand at 1174.063 kb on + strand at 1174.063 kb on + strand at 1174.066 kb on + strand at 1174.066 kb on + strand at 1174.090 kb on + strand at 1174.189 kb on + strand at 1174.189 kb on + strand at 1174.190 kb on - strand at 1174.190 kb on - strand at 1174.219 kb on - strand, within PP_1029 at 1174.389 kb on + strand, within PP_1029 at 1174.390 kb on - strand, within PP_1029 at 1174.390 kb on - strand, within PP_1029 at 1174.390 kb on - strand, within PP_1029 at 1174.465 kb on + strand, within PP_1029 at 1174.466 kb on - strand, within PP_1029 at 1174.466 kb on - strand, within PP_1029 at 1174.474 kb on + strand, within PP_1029 at 1174.585 kb on + strand, within PP_1029 at 1174.595 kb on - strand, within PP_1029 at 1174.629 kb on - strand, within PP_1029 at 1174.629 kb on - strand, within PP_1029 at 1174.629 kb on - strand, within PP_1029 at 1174.662 kb on + strand, within PP_1029 at 1174.663 kb on - strand, within PP_1029 at 1174.668 kb on - strand, within PP_1029 at 1174.702 kb on + strand, within PP_1029 at 1174.703 kb on - strand, within PP_1029 at 1174.703 kb on - strand, within PP_1029 at 1174.864 kb on + strand at 1175.175 kb on + strand, within PP_1030 at 1175.175 kb on + strand, within PP_1030 at 1175.175 kb on + strand, within PP_1030 at 1175.175 kb on + strand, within PP_1030 at 1175.175 kb on + strand, within PP_1030 at 1175.175 kb on + strand, within PP_1030 at 1175.175 kb on + strand, within PP_1030 at 1175.176 kb on - strand, within PP_1030 at 1175.176 kb on - strand, within PP_1030 at 1175.176 kb on - strand, within PP_1030 at 1175.176 kb on - strand, within PP_1030 at 1175.176 kb on - strand, within PP_1030 at 1175.178 kb on - strand, within PP_1030 at 1175.292 kb on - strand, within PP_1030 at 1175.292 kb on - strand, within PP_1030 at 1175.292 kb on - strand, within PP_1030 at 1175.292 kb on - strand, within PP_1030 at 1175.337 kb on + strand, within PP_1030 at 1175.338 kb on - strand, within PP_1030 at 1175.465 kb on - strand at 1175.495 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 48hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 30% DO with 20% v/v dodecane overlay remove 1,173,946 + -0.4 1,173,946 + +0.6 1,173,947 - -1.4 1,174,063 + +1.3 1,174,063 + +1.0 1,174,066 + -0.6 1,174,066 + -0.4 1,174,090 + -0.7 1,174,189 + +1.4 1,174,189 + +0.9 1,174,190 - -0.7 1,174,190 - +0.9 1,174,219 - PP_1029 0.13 +0.3 1,174,389 + PP_1029 0.35 -0.1 1,174,390 - PP_1029 0.35 +0.6 1,174,390 - PP_1029 0.35 +0.1 1,174,390 - PP_1029 0.35 +0.7 1,174,465 + PP_1029 0.45 -0.5 1,174,466 - PP_1029 0.45 +0.6 1,174,466 - PP_1029 0.45 +0.9 1,174,474 + PP_1029 0.46 -1.0 1,174,585 + PP_1029 0.61 -0.6 1,174,595 - PP_1029 0.62 -0.3 1,174,629 - PP_1029 0.67 -0.3 1,174,629 - PP_1029 0.67 -0.5 1,174,629 - PP_1029 0.67 -1.7 1,174,662 + PP_1029 0.71 -0.2 1,174,663 - PP_1029 0.71 +0.4 1,174,668 - PP_1029 0.72 -0.6 1,174,702 + PP_1029 0.77 -0.1 1,174,703 - PP_1029 0.77 +0.8 1,174,703 - PP_1029 0.77 -0.1 1,174,864 + +1.1 1,175,175 + PP_1030 0.45 -1.3 1,175,175 + PP_1030 0.45 +0.6 1,175,175 + PP_1030 0.45 +0.6 1,175,175 + PP_1030 0.45 -0.1 1,175,175 + PP_1030 0.45 -1.0 1,175,175 + PP_1030 0.45 +0.2 1,175,175 + PP_1030 0.45 +0.4 1,175,176 - PP_1030 0.45 -0.5 1,175,176 - PP_1030 0.45 +0.1 1,175,176 - PP_1030 0.45 +0.2 1,175,176 - PP_1030 0.45 +1.1 1,175,176 - PP_1030 0.45 -0.2 1,175,178 - PP_1030 0.45 -0.6 1,175,292 - PP_1030 0.66 +1.6 1,175,292 - PP_1030 0.66 +0.9 1,175,292 - PP_1030 0.66 +0.3 1,175,292 - PP_1030 0.66 +0.4 1,175,337 + PP_1030 0.74 +0.6 1,175,338 - PP_1030 0.74 -0.9 1,175,465 - +0.2 1,175,495 + +1.0
Or see this region's nucleotide sequence