Strain Fitness in Escherichia coli BW25113 around b2243

Experiment: LB Anaerobic with Furaltadone 0.000015625 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntglpB and glpC overlap by 4 nucleotidesglpC and yfaD are separated by 192 nucleotides b2242: glpB - anaerobic glycerol-3-phosphate dehydrogenase subunit B (NCBI), at 2,352,287 to 2,353,546 glpB b2243: glpC - sn-glycerol-3-phosphate dehydrogenase (anaerobic), small subunit (NCBI), at 2,353,543 to 2,354,733 glpC b2244: yfaD - hypothetical protein (NCBI), at 2,354,926 to 2,355,825 yfaD Position (kb) 2353 2354 2355Strain fitness (log2 ratio) -2 -1 0 1 2at 2352.547 kb on + strand, within glpBat 2352.631 kb on + strand, within glpBat 2352.650 kb on + strand, within glpBat 2352.650 kb on + strand, within glpBat 2352.787 kb on - strand, within glpBat 2353.281 kb on + strand, within glpBat 2353.291 kb on + strand, within glpBat 2353.291 kb on + strand, within glpBat 2353.326 kb on - strand, within glpBat 2353.326 kb on - strand, within glpBat 2353.379 kb on - strand, within glpBat 2353.400 kb on + strand, within glpBat 2353.400 kb on + strand, within glpBat 2353.436 kb on + strandat 2353.436 kb on + strandat 2353.446 kb on + strandat 2353.575 kb on - strandat 2353.575 kb on - strandat 2353.577 kb on + strandat 2353.577 kb on + strandat 2353.615 kb on - strandat 2353.684 kb on + strand, within glpCat 2353.686 kb on + strand, within glpCat 2353.696 kb on - strand, within glpCat 2353.696 kb on - strand, within glpCat 2353.767 kb on + strand, within glpCat 2353.812 kb on + strand, within glpCat 2353.853 kb on + strand, within glpCat 2353.853 kb on + strand, within glpCat 2353.871 kb on - strand, within glpCat 2354.115 kb on - strand, within glpCat 2354.125 kb on - strand, within glpCat 2354.125 kb on - strand, within glpCat 2354.169 kb on + strand, within glpCat 2354.278 kb on - strand, within glpCat 2354.438 kb on - strand, within glpCat 2354.534 kb on + strand, within glpCat 2354.542 kb on - strand, within glpCat 2354.591 kb on + strand, within glpCat 2354.676 kb on + strandat 2354.676 kb on + strandat 2354.684 kb on - strandat 2354.752 kb on + strandat 2354.793 kb on - strandat 2354.793 kb on - strandat 2354.892 kb on - strandat 2354.926 kb on - strandat 2354.926 kb on - strandat 2354.941 kb on - strandat 2354.941 kb on - strandat 2355.020 kb on + strand, within yfaDat 2355.149 kb on + strand, within yfaDat 2355.322 kb on - strand, within yfaDat 2355.322 kb on - strand, within yfaDat 2355.322 kb on - strand, within yfaDat 2355.369 kb on + strand, within yfaDat 2355.396 kb on + strand, within yfaDat 2355.438 kb on + strand, within yfaDat 2355.438 kb on + strand, within yfaDat 2355.448 kb on + strand, within yfaDat 2355.448 kb on + strand, within yfaDat 2355.448 kb on + strand, within yfaDat 2355.499 kb on + strand, within yfaDat 2355.665 kb on - strand, within yfaD

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Anaerobic with Furaltadone 0.000015625 mM
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2,352,547 + glpB b2242 0.21 -1.2
2,352,631 + glpB b2242 0.27 -0.8
2,352,650 + glpB b2242 0.29 -1.4
2,352,650 + glpB b2242 0.29 -0.8
2,352,787 - glpB b2242 0.40 -0.5
2,353,281 + glpB b2242 0.79 -0.3
2,353,291 + glpB b2242 0.80 -1.4
2,353,291 + glpB b2242 0.80 +1.2
2,353,326 - glpB b2242 0.82 +1.3
2,353,326 - glpB b2242 0.82 +1.6
2,353,379 - glpB b2242 0.87 -1.0
2,353,400 + glpB b2242 0.88 +0.3
2,353,400 + glpB b2242 0.88 +0.3
2,353,436 + -1.0
2,353,436 + +0.2
2,353,446 + -2.0
2,353,575 - +1.6
2,353,575 - -0.1
2,353,577 + +0.2
2,353,577 + -0.0
2,353,615 - +1.4
2,353,684 + glpC b2243 0.12 +0.9
2,353,686 + glpC b2243 0.12 -0.4
2,353,696 - glpC b2243 0.13 -0.0
2,353,696 - glpC b2243 0.13 -0.4
2,353,767 + glpC b2243 0.19 -0.8
2,353,812 + glpC b2243 0.23 -1.0
2,353,853 + glpC b2243 0.26 -0.5
2,353,853 + glpC b2243 0.26 -0.8
2,353,871 - glpC b2243 0.28 -1.4
2,354,115 - glpC b2243 0.48 -0.3
2,354,125 - glpC b2243 0.49 -0.0
2,354,125 - glpC b2243 0.49 +1.3
2,354,169 + glpC b2243 0.53 -0.9
2,354,278 - glpC b2243 0.62 -1.4
2,354,438 - glpC b2243 0.75 -0.8
2,354,534 + glpC b2243 0.83 -1.6
2,354,542 - glpC b2243 0.84 -0.4
2,354,591 + glpC b2243 0.88 -1.0
2,354,676 + -0.3
2,354,676 + +0.1
2,354,684 - -1.9
2,354,752 + +0.4
2,354,793 - -1.2
2,354,793 - +0.6
2,354,892 - +0.6
2,354,926 - +0.1
2,354,926 - -0.4
2,354,941 - +1.2
2,354,941 - +0.6
2,355,020 + yfaD b2244 0.10 -0.4
2,355,149 + yfaD b2244 0.25 +1.9
2,355,322 - yfaD b2244 0.44 +0.3
2,355,322 - yfaD b2244 0.44 +0.0
2,355,322 - yfaD b2244 0.44 +0.4
2,355,369 + yfaD b2244 0.49 +0.5
2,355,396 + yfaD b2244 0.52 -0.5
2,355,438 + yfaD b2244 0.57 +0.2
2,355,438 + yfaD b2244 0.57 -2.0
2,355,448 + yfaD b2244 0.58 +0.2
2,355,448 + yfaD b2244 0.58 -0.5
2,355,448 + yfaD b2244 0.58 +0.3
2,355,499 + yfaD b2244 0.64 +0.4
2,355,665 - yfaD b2244 0.82 -2.1

Or see this region's nucleotide sequence