Strain Fitness in Escherichia coli BW25113 around b2224

Experiment: LB Anaerobic with Furaltadone 0.000015625 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntatoE and atoB are separated by 30 nucleotidesatoB and yfaP are separated by 73 nucleotidesyfaP and yfaQ are separated by 4 nucleotides b2223: atoE - short chain fatty acid transporter (NCBI), at 2,322,778 to 2,324,100 atoE b2224: atoB - acetyl-CoA acetyltransferase (NCBI), at 2,324,131 to 2,325,315 atoB b2225: yfaP - hypothetical protein (NCBI), at 2,325,389 to 2,326,165 yfaP b2226: yfaQ - hypothetical protein (NCBI), at 2,326,170 to 2,327,819 yfaQ Position (kb) 2324 2325 2326Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 2323.139 kb on + strand, within atoEat 2323.194 kb on - strand, within atoEat 2323.260 kb on + strand, within atoEat 2323.321 kb on - strand, within atoEat 2323.321 kb on - strand, within atoEat 2323.403 kb on + strand, within atoEat 2323.417 kb on - strand, within atoEat 2323.417 kb on - strand, within atoEat 2323.426 kb on - strand, within atoEat 2323.475 kb on - strand, within atoEat 2323.601 kb on + strand, within atoEat 2323.601 kb on + strand, within atoEat 2323.608 kb on + strand, within atoEat 2323.873 kb on + strand, within atoEat 2324.037 kb on + strandat 2324.037 kb on + strandat 2324.037 kb on + strandat 2324.148 kb on + strandat 2324.307 kb on - strand, within atoBat 2324.469 kb on + strand, within atoBat 2324.539 kb on - strand, within atoBat 2324.547 kb on + strand, within atoBat 2324.547 kb on + strand, within atoBat 2324.549 kb on + strand, within atoBat 2324.585 kb on - strand, within atoBat 2324.735 kb on + strand, within atoBat 2324.886 kb on - strand, within atoBat 2325.002 kb on - strand, within atoBat 2325.006 kb on - strand, within atoBat 2325.077 kb on - strand, within atoBat 2325.094 kb on + strand, within atoBat 2325.215 kb on - strandat 2325.222 kb on - strandat 2325.224 kb on - strandat 2325.224 kb on - strandat 2325.226 kb on + strandat 2325.354 kb on + strandat 2325.354 kb on + strandat 2325.393 kb on - strandat 2325.393 kb on - strandat 2325.488 kb on + strand, within yfaPat 2325.526 kb on + strand, within yfaPat 2325.537 kb on - strand, within yfaPat 2325.555 kb on - strand, within yfaPat 2325.623 kb on - strand, within yfaPat 2325.623 kb on - strand, within yfaPat 2325.656 kb on - strand, within yfaPat 2325.756 kb on + strand, within yfaPat 2325.759 kb on - strand, within yfaPat 2325.970 kb on + strand, within yfaPat 2325.970 kb on + strand, within yfaPat 2326.112 kb on - strandat 2326.112 kb on - strandat 2326.209 kb on + strandat 2326.209 kb on + strandat 2326.237 kb on - strandat 2326.286 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Anaerobic with Furaltadone 0.000015625 mM
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2,323,139 + atoE b2223 0.27 -0.4
2,323,194 - atoE b2223 0.31 -0.4
2,323,260 + atoE b2223 0.36 +0.4
2,323,321 - atoE b2223 0.41 +0.9
2,323,321 - atoE b2223 0.41 -0.3
2,323,403 + atoE b2223 0.47 -0.9
2,323,417 - atoE b2223 0.48 +0.4
2,323,417 - atoE b2223 0.48 -0.0
2,323,426 - atoE b2223 0.49 +0.1
2,323,475 - atoE b2223 0.53 +1.1
2,323,601 + atoE b2223 0.62 -3.2
2,323,601 + atoE b2223 0.62 +1.1
2,323,608 + atoE b2223 0.63 +0.8
2,323,873 + atoE b2223 0.83 -0.4
2,324,037 + -0.5
2,324,037 + +1.2
2,324,037 + -0.3
2,324,148 + -0.2
2,324,307 - atoB b2224 0.15 -0.5
2,324,469 + atoB b2224 0.29 +1.0
2,324,539 - atoB b2224 0.34 +0.4
2,324,547 + atoB b2224 0.35 -0.8
2,324,547 + atoB b2224 0.35 +0.4
2,324,549 + atoB b2224 0.35 -0.4
2,324,585 - atoB b2224 0.38 +0.2
2,324,735 + atoB b2224 0.51 +0.0
2,324,886 - atoB b2224 0.64 +2.2
2,325,002 - atoB b2224 0.74 -1.2
2,325,006 - atoB b2224 0.74 -0.3
2,325,077 - atoB b2224 0.80 -0.1
2,325,094 + atoB b2224 0.81 -0.3
2,325,215 - -0.0
2,325,222 - +0.6
2,325,224 - +1.6
2,325,224 - -1.0
2,325,226 + -0.6
2,325,354 + +0.6
2,325,354 + -2.0
2,325,393 - -0.1
2,325,393 - -0.4
2,325,488 + yfaP b2225 0.13 -0.4
2,325,526 + yfaP b2225 0.18 +0.6
2,325,537 - yfaP b2225 0.19 -0.3
2,325,555 - yfaP b2225 0.21 +1.3
2,325,623 - yfaP b2225 0.30 -0.7
2,325,623 - yfaP b2225 0.30 -0.2
2,325,656 - yfaP b2225 0.34 -2.2
2,325,756 + yfaP b2225 0.47 -0.2
2,325,759 - yfaP b2225 0.48 +1.0
2,325,970 + yfaP b2225 0.75 -0.4
2,325,970 + yfaP b2225 0.75 +0.4
2,326,112 - -0.0
2,326,112 - -2.2
2,326,209 + -1.2
2,326,209 + +0.6
2,326,237 - -0.8
2,326,286 - +0.0

Or see this region's nucleotide sequence