Strain Fitness in Escherichia coli BW25113 around b1929

Experiment: LB Anaerobic with Furaltadone 0.000015625 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntamyA and yedD are separated by 33 nucleotidesyedD and yedE are separated by 186 nucleotidesyedE and yedF overlap by 4 nucleotidesyedF and yedK are separated by 108 nucleotides b1927: amyA - cytoplasmic alpha-amylase (NCBI), at 2,004,180 to 2,005,667 amyA b1928: yedD - hypothetical protein (NCBI), at 2,005,701 to 2,006,114 yedD b1929: yedE - predicted inner membrane protein (NCBI), at 2,006,301 to 2,007,506 yedE b1930: yedF - hypothetical protein (NCBI), at 2,007,503 to 2,007,736 yedF b1931: yedK - hypothetical protein (NCBI), at 2,007,845 to 2,008,513 yedK Position (kb) 2006 2007 2008Strain fitness (log2 ratio) -2 -1 0 1 2at 2005.308 kb on + strand, within amyAat 2005.308 kb on + strand, within amyAat 2005.310 kb on + strand, within amyAat 2005.410 kb on - strand, within amyAat 2005.410 kb on - strand, within amyAat 2005.443 kb on - strand, within amyAat 2005.591 kb on - strandat 2005.633 kb on + strandat 2005.633 kb on + strandat 2005.659 kb on - strandat 2005.659 kb on - strandat 2005.729 kb on + strandat 2005.787 kb on - strand, within yedDat 2005.788 kb on + strand, within yedDat 2005.801 kb on - strand, within yedDat 2005.801 kb on - strand, within yedDat 2005.809 kb on - strand, within yedDat 2005.827 kb on - strand, within yedDat 2005.953 kb on + strand, within yedDat 2005.969 kb on - strand, within yedDat 2006.256 kb on - strandat 2006.284 kb on + strandat 2006.393 kb on - strandat 2006.393 kb on - strandat 2006.417 kb on + strandat 2006.417 kb on + strandat 2006.457 kb on - strand, within yedEat 2006.508 kb on + strand, within yedEat 2006.605 kb on - strand, within yedEat 2006.732 kb on - strand, within yedEat 2006.767 kb on - strand, within yedEat 2006.772 kb on - strand, within yedEat 2006.951 kb on - strand, within yedEat 2006.989 kb on + strand, within yedEat 2007.062 kb on + strand, within yedEat 2007.062 kb on + strand, within yedEat 2007.131 kb on + strand, within yedEat 2007.131 kb on + strand, within yedEat 2007.244 kb on - strand, within yedEat 2007.257 kb on - strand, within yedEat 2007.261 kb on + strand, within yedEat 2007.263 kb on - strand, within yedEat 2007.314 kb on + strand, within yedEat 2007.541 kb on + strand, within yedFat 2007.542 kb on + strand, within yedFat 2007.627 kb on + strand, within yedFat 2007.628 kb on - strand, within yedFat 2007.630 kb on + strand, within yedFat 2007.656 kb on - strand, within yedFat 2007.710 kb on + strand, within yedFat 2007.744 kb on + strandat 2007.763 kb on - strandat 2007.767 kb on - strandat 2007.775 kb on - strandat 2007.931 kb on + strand, within yedKat 2008.001 kb on - strand, within yedKat 2008.003 kb on + strand, within yedKat 2008.003 kb on + strand, within yedKat 2008.095 kb on + strand, within yedKat 2008.119 kb on - strand, within yedKat 2008.295 kb on - strand, within yedKat 2008.389 kb on + strand, within yedKat 2008.506 kb on + strandat 2008.506 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Anaerobic with Furaltadone 0.000015625 mM
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2,005,308 + amyA b1927 0.76 -0.7
2,005,308 + amyA b1927 0.76 +0.2
2,005,310 + amyA b1927 0.76 -1.0
2,005,410 - amyA b1927 0.83 -1.0
2,005,410 - amyA b1927 0.83 +0.3
2,005,443 - amyA b1927 0.85 +0.2
2,005,591 - +0.6
2,005,633 + +1.2
2,005,633 + +2.0
2,005,659 - -0.4
2,005,659 - +0.6
2,005,729 + +0.7
2,005,787 - yedD b1928 0.21 +0.6
2,005,788 + yedD b1928 0.21 -0.9
2,005,801 - yedD b1928 0.24 -0.7
2,005,801 - yedD b1928 0.24 +1.0
2,005,809 - yedD b1928 0.26 -1.0
2,005,827 - yedD b1928 0.30 +1.0
2,005,953 + yedD b1928 0.61 -0.6
2,005,969 - yedD b1928 0.65 -1.8
2,006,256 - +0.9
2,006,284 + +0.3
2,006,393 - +0.7
2,006,393 - +1.3
2,006,417 + -0.8
2,006,417 + +0.5
2,006,457 - yedE b1929 0.13 +1.1
2,006,508 + yedE b1929 0.17 +1.3
2,006,605 - yedE b1929 0.25 +0.6
2,006,732 - yedE b1929 0.36 +0.9
2,006,767 - yedE b1929 0.39 -0.3
2,006,772 - yedE b1929 0.39 +1.7
2,006,951 - yedE b1929 0.54 +0.4
2,006,989 + yedE b1929 0.57 +0.1
2,007,062 + yedE b1929 0.63 -1.5
2,007,062 + yedE b1929 0.63 +0.8
2,007,131 + yedE b1929 0.69 +1.0
2,007,131 + yedE b1929 0.69 +0.3
2,007,244 - yedE b1929 0.78 +1.2
2,007,257 - yedE b1929 0.79 -0.4
2,007,261 + yedE b1929 0.80 +0.9
2,007,263 - yedE b1929 0.80 -0.0
2,007,314 + yedE b1929 0.84 +0.7
2,007,541 + yedF b1930 0.16 +1.4
2,007,542 + yedF b1930 0.17 +0.5
2,007,627 + yedF b1930 0.53 -0.0
2,007,628 - yedF b1930 0.53 +1.5
2,007,630 + yedF b1930 0.54 -0.1
2,007,656 - yedF b1930 0.65 -1.1
2,007,710 + yedF b1930 0.88 -0.7
2,007,744 + +0.2
2,007,763 - +1.0
2,007,767 - +0.3
2,007,775 - -1.0
2,007,931 + yedK b1931 0.13 +0.5
2,008,001 - yedK b1931 0.23 +0.4
2,008,003 + yedK b1931 0.24 -0.7
2,008,003 + yedK b1931 0.24 +0.8
2,008,095 + yedK b1931 0.37 -1.7
2,008,119 - yedK b1931 0.41 -1.4
2,008,295 - yedK b1931 0.67 -1.7
2,008,389 + yedK b1931 0.81 -0.6
2,008,506 + +1.6
2,008,506 + -1.0

Or see this region's nucleotide sequence