Experiment: LB Anaerobic with Furaltadone 0.000015625 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt amyA and yedD are separated by 33 nucleotides yedD and yedE are separated by 186 nucleotides yedE and yedF overlap by 4 nucleotides yedF and yedK are separated by 108 nucleotides
b1927: amyA - cytoplasmic alpha-amylase (NCBI), at 2,004,180 to 2,005,667
amyA
b1928: yedD - hypothetical protein (NCBI), at 2,005,701 to 2,006,114
yedD
b1929: yedE - predicted inner membrane protein (NCBI), at 2,006,301 to 2,007,506
yedE
b1930: yedF - hypothetical protein (NCBI), at 2,007,503 to 2,007,736
yedF
b1931: yedK - hypothetical protein (NCBI), at 2,007,845 to 2,008,513
yedK
Position (kb)
2006
2007
2008 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2005.308 kb on + strand, within amyA at 2005.308 kb on + strand, within amyA at 2005.310 kb on + strand, within amyA at 2005.410 kb on - strand, within amyA at 2005.410 kb on - strand, within amyA at 2005.443 kb on - strand, within amyA at 2005.591 kb on - strand at 2005.633 kb on + strand at 2005.633 kb on + strand at 2005.659 kb on - strand at 2005.659 kb on - strand at 2005.729 kb on + strand at 2005.787 kb on - strand, within yedD at 2005.788 kb on + strand, within yedD at 2005.801 kb on - strand, within yedD at 2005.801 kb on - strand, within yedD at 2005.809 kb on - strand, within yedD at 2005.827 kb on - strand, within yedD at 2005.953 kb on + strand, within yedD at 2005.969 kb on - strand, within yedD at 2006.256 kb on - strand at 2006.284 kb on + strand at 2006.393 kb on - strand at 2006.393 kb on - strand at 2006.417 kb on + strand at 2006.417 kb on + strand at 2006.457 kb on - strand, within yedE at 2006.508 kb on + strand, within yedE at 2006.605 kb on - strand, within yedE at 2006.732 kb on - strand, within yedE at 2006.767 kb on - strand, within yedE at 2006.772 kb on - strand, within yedE at 2006.951 kb on - strand, within yedE at 2006.989 kb on + strand, within yedE at 2007.062 kb on + strand, within yedE at 2007.062 kb on + strand, within yedE at 2007.131 kb on + strand, within yedE at 2007.131 kb on + strand, within yedE at 2007.244 kb on - strand, within yedE at 2007.257 kb on - strand, within yedE at 2007.261 kb on + strand, within yedE at 2007.263 kb on - strand, within yedE at 2007.314 kb on + strand, within yedE at 2007.541 kb on + strand, within yedF at 2007.542 kb on + strand, within yedF at 2007.627 kb on + strand, within yedF at 2007.628 kb on - strand, within yedF at 2007.630 kb on + strand, within yedF at 2007.656 kb on - strand, within yedF at 2007.710 kb on + strand, within yedF at 2007.744 kb on + strand at 2007.763 kb on - strand at 2007.767 kb on - strand at 2007.775 kb on - strand at 2007.931 kb on + strand, within yedK at 2008.001 kb on - strand, within yedK at 2008.003 kb on + strand, within yedK at 2008.003 kb on + strand, within yedK at 2008.095 kb on + strand, within yedK at 2008.119 kb on - strand, within yedK at 2008.295 kb on - strand, within yedK at 2008.389 kb on + strand, within yedK at 2008.506 kb on + strand at 2008.506 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Furaltadone 0.000015625 mM remove 2,005,308 + amyA b1927 0.76 -0.7 2,005,308 + amyA b1927 0.76 +0.2 2,005,310 + amyA b1927 0.76 -1.0 2,005,410 - amyA b1927 0.83 -1.0 2,005,410 - amyA b1927 0.83 +0.3 2,005,443 - amyA b1927 0.85 +0.2 2,005,591 - +0.6 2,005,633 + +1.2 2,005,633 + +2.0 2,005,659 - -0.4 2,005,659 - +0.6 2,005,729 + +0.7 2,005,787 - yedD b1928 0.21 +0.6 2,005,788 + yedD b1928 0.21 -0.9 2,005,801 - yedD b1928 0.24 -0.7 2,005,801 - yedD b1928 0.24 +1.0 2,005,809 - yedD b1928 0.26 -1.0 2,005,827 - yedD b1928 0.30 +1.0 2,005,953 + yedD b1928 0.61 -0.6 2,005,969 - yedD b1928 0.65 -1.8 2,006,256 - +0.9 2,006,284 + +0.3 2,006,393 - +0.7 2,006,393 - +1.3 2,006,417 + -0.8 2,006,417 + +0.5 2,006,457 - yedE b1929 0.13 +1.1 2,006,508 + yedE b1929 0.17 +1.3 2,006,605 - yedE b1929 0.25 +0.6 2,006,732 - yedE b1929 0.36 +0.9 2,006,767 - yedE b1929 0.39 -0.3 2,006,772 - yedE b1929 0.39 +1.7 2,006,951 - yedE b1929 0.54 +0.4 2,006,989 + yedE b1929 0.57 +0.1 2,007,062 + yedE b1929 0.63 -1.5 2,007,062 + yedE b1929 0.63 +0.8 2,007,131 + yedE b1929 0.69 +1.0 2,007,131 + yedE b1929 0.69 +0.3 2,007,244 - yedE b1929 0.78 +1.2 2,007,257 - yedE b1929 0.79 -0.4 2,007,261 + yedE b1929 0.80 +0.9 2,007,263 - yedE b1929 0.80 -0.0 2,007,314 + yedE b1929 0.84 +0.7 2,007,541 + yedF b1930 0.16 +1.4 2,007,542 + yedF b1930 0.17 +0.5 2,007,627 + yedF b1930 0.53 -0.0 2,007,628 - yedF b1930 0.53 +1.5 2,007,630 + yedF b1930 0.54 -0.1 2,007,656 - yedF b1930 0.65 -1.1 2,007,710 + yedF b1930 0.88 -0.7 2,007,744 + +0.2 2,007,763 - +1.0 2,007,767 - +0.3 2,007,775 - -1.0 2,007,931 + yedK b1931 0.13 +0.5 2,008,001 - yedK b1931 0.23 +0.4 2,008,003 + yedK b1931 0.24 -0.7 2,008,003 + yedK b1931 0.24 +0.8 2,008,095 + yedK b1931 0.37 -1.7 2,008,119 - yedK b1931 0.41 -1.4 2,008,295 - yedK b1931 0.67 -1.7 2,008,389 + yedK b1931 0.81 -0.6 2,008,506 + +1.6 2,008,506 + -1.0
Or see this region's nucleotide sequence