Experiment: LB Anaerobic with Furaltadone 0.000015625 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt entC and entE are separated by 9 nucleotides entE and entB are separated by 13 nucleotides entB and entA overlap by 1 nucleotides
b0593: entC - isochorismate synthase (NCBI), at 624,108 to 625,283
entC
b0594: entE - 2,3-dihydroxybenzoate-AMP ligase component of enterobactin synthase multienzyme complex (NCBI), at 625,293 to 626,903
entE
b0595: entB - isochorismatase (NCBI), at 626,917 to 627,774
entB
b0596: entA - 2,3-dihydroxybenzoate-2,3-dehydrogenase (NCBI), at 627,774 to 628,520
entA
Position (kb) 625
626
627 Strain fitness (log2 ratio) -2
-1
0
1
2 at 624.428 kb on - strand, within entC at 624.428 kb on - strand, within entC at 624.490 kb on + strand, within entC at 624.547 kb on - strand, within entC at 624.580 kb on - strand, within entC at 624.691 kb on + strand, within entC at 624.691 kb on + strand, within entC at 624.699 kb on - strand, within entC at 624.699 kb on - strand, within entC at 624.699 kb on - strand, within entC at 624.831 kb on - strand, within entC at 624.875 kb on + strand, within entC at 624.947 kb on + strand, within entC at 624.947 kb on + strand, within entC at 624.984 kb on + strand, within entC at 624.984 kb on + strand, within entC at 625.127 kb on - strand, within entC at 625.365 kb on - strand at 625.365 kb on - strand at 625.401 kb on + strand at 625.401 kb on + strand at 625.433 kb on + strand at 625.593 kb on + strand, within entE at 625.604 kb on + strand, within entE at 625.604 kb on + strand, within entE at 625.617 kb on - strand, within entE at 625.688 kb on + strand, within entE at 625.744 kb on + strand, within entE at 625.744 kb on + strand, within entE at 625.746 kb on + strand, within entE at 625.900 kb on + strand, within entE at 625.958 kb on + strand, within entE at 625.964 kb on - strand, within entE at 625.964 kb on - strand, within entE at 625.971 kb on - strand, within entE at 626.073 kb on + strand, within entE at 626.303 kb on - strand, within entE at 626.303 kb on - strand, within entE at 626.369 kb on - strand, within entE at 626.487 kb on - strand, within entE at 626.643 kb on + strand, within entE at 626.643 kb on + strand, within entE at 626.751 kb on + strand at 626.751 kb on + strand at 626.851 kb on - strand at 626.896 kb on - strand at 626.908 kb on - strand at 626.908 kb on - strand at 626.937 kb on + strand at 626.937 kb on + strand at 627.113 kb on + strand, within entB at 627.113 kb on + strand, within entB at 627.128 kb on + strand, within entB at 627.128 kb on + strand, within entB at 627.146 kb on - strand, within entB at 627.146 kb on - strand, within entB at 627.146 kb on - strand, within entB at 627.146 kb on - strand, within entB at 627.216 kb on - strand, within entB at 627.216 kb on - strand, within entB at 627.367 kb on + strand, within entB at 627.437 kb on - strand, within entB at 627.437 kb on - strand, within entB at 627.550 kb on - strand, within entB at 627.610 kb on - strand, within entB at 627.619 kb on + strand, within entB at 627.619 kb on + strand, within entB at 627.629 kb on - strand, within entB at 627.706 kb on - strand at 627.751 kb on + strand at 627.825 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Furaltadone 0.000015625 mM remove 624,428 - entC b0593 0.27 +0.8 624,428 - entC b0593 0.27 +0.6 624,490 + entC b0593 0.32 +0.5 624,547 - entC b0593 0.37 +0.5 624,580 - entC b0593 0.40 -0.3 624,691 + entC b0593 0.50 +0.5 624,691 + entC b0593 0.50 +0.4 624,699 - entC b0593 0.50 +0.4 624,699 - entC b0593 0.50 +0.2 624,699 - entC b0593 0.50 -1.2 624,831 - entC b0593 0.61 +0.1 624,875 + entC b0593 0.65 +0.2 624,947 + entC b0593 0.71 +1.8 624,947 + entC b0593 0.71 +0.2 624,984 + entC b0593 0.74 +0.2 624,984 + entC b0593 0.74 +1.0 625,127 - entC b0593 0.87 -0.0 625,365 - -0.5 625,365 - +0.9 625,401 + -1.2 625,401 + -1.1 625,433 + -0.5 625,593 + entE b0594 0.19 +0.7 625,604 + entE b0594 0.19 +0.9 625,604 + entE b0594 0.19 +0.6 625,617 - entE b0594 0.20 +1.8 625,688 + entE b0594 0.25 +0.2 625,744 + entE b0594 0.28 -0.2 625,744 + entE b0594 0.28 +1.1 625,746 + entE b0594 0.28 +0.2 625,900 + entE b0594 0.38 +2.0 625,958 + entE b0594 0.41 -1.2 625,964 - entE b0594 0.42 +1.7 625,964 - entE b0594 0.42 +1.1 625,971 - entE b0594 0.42 -0.5 626,073 + entE b0594 0.48 +0.2 626,303 - entE b0594 0.63 -2.0 626,303 - entE b0594 0.63 +0.1 626,369 - entE b0594 0.67 +1.3 626,487 - entE b0594 0.74 -0.6 626,643 + entE b0594 0.84 -1.2 626,643 + entE b0594 0.84 +0.6 626,751 + +0.1 626,751 + +1.1 626,851 - +1.9 626,896 - -0.5 626,908 - -0.1 626,908 - -0.4 626,937 + -0.8 626,937 + -0.6 627,113 + entB b0595 0.23 +0.6 627,113 + entB b0595 0.23 +1.5 627,128 + entB b0595 0.25 -0.0 627,128 + entB b0595 0.25 -1.2 627,146 - entB b0595 0.27 -1.7 627,146 - entB b0595 0.27 +0.2 627,146 - entB b0595 0.27 -2.7 627,146 - entB b0595 0.27 +0.0 627,216 - entB b0595 0.35 +0.1 627,216 - entB b0595 0.35 +0.2 627,367 + entB b0595 0.52 +0.8 627,437 - entB b0595 0.61 -0.1 627,437 - entB b0595 0.61 +0.3 627,550 - entB b0595 0.74 -0.4 627,610 - entB b0595 0.81 -0.6 627,619 + entB b0595 0.82 -0.1 627,619 + entB b0595 0.82 +0.6 627,629 - entB b0595 0.83 -0.2 627,706 - +0.9 627,751 + -0.7 627,825 + +0.1
Or see this region's nucleotide sequence