Experiment: KB with Sinomenine 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_5014 and Psyr_5015 are separated by 81 nucleotides Psyr_5015 and Psyr_5016 are separated by 419 nucleotides
Psyr_5014: Psyr_5014 - Acyl-CoA dehydrogenase, C-terminal:Acyl-CoA dehydrogenase, central region:Acyl-CoA dehydrogenase, N-terminal, at 5,955,010 to 5,956,191
_5014
Psyr_5015: Psyr_5015 - transcriptional regulator, LysR family, at 5,956,273 to 5,957,259
_5015
Psyr_5016: Psyr_5016 - Alanine dehydrogenase/PNT, C-terminal:Alanine dehydrogenase/PNT, N-terminal, at 5,957,679 to 5,958,800
_5016
Position (kb) 5956
5957
5958 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 5955.374 kb on + strand, within Psyr_5014 at 5955.374 kb on + strand, within Psyr_5014 at 5955.375 kb on - strand, within Psyr_5014 at 5955.821 kb on + strand, within Psyr_5014 at 5955.821 kb on + strand, within Psyr_5014 at 5955.821 kb on + strand, within Psyr_5014 at 5955.821 kb on + strand, within Psyr_5014 at 5955.821 kb on + strand, within Psyr_5014 at 5955.822 kb on - strand, within Psyr_5014 at 5955.822 kb on - strand, within Psyr_5014 at 5955.822 kb on - strand, within Psyr_5014 at 5955.944 kb on + strand, within Psyr_5014 at 5955.953 kb on + strand, within Psyr_5014 at 5955.953 kb on + strand, within Psyr_5014 at 5955.953 kb on + strand, within Psyr_5014 at 5955.953 kb on + strand, within Psyr_5014 at 5955.953 kb on + strand, within Psyr_5014 at 5955.953 kb on + strand, within Psyr_5014 at 5955.953 kb on + strand, within Psyr_5014 at 5955.953 kb on + strand, within Psyr_5014 at 5955.954 kb on - strand, within Psyr_5014 at 5955.954 kb on - strand, within Psyr_5014 at 5955.954 kb on - strand, within Psyr_5014 at 5956.231 kb on + strand at 5956.231 kb on + strand at 5956.266 kb on - strand at 5956.342 kb on + strand at 5956.359 kb on - strand at 5956.549 kb on + strand, within Psyr_5015 at 5956.549 kb on + strand, within Psyr_5015 at 5956.549 kb on + strand, within Psyr_5015 at 5956.550 kb on - strand, within Psyr_5015 at 5956.550 kb on - strand, within Psyr_5015 at 5956.550 kb on - strand, within Psyr_5015 at 5956.550 kb on - strand, within Psyr_5015 at 5956.551 kb on + strand, within Psyr_5015 at 5956.551 kb on + strand, within Psyr_5015 at 5956.551 kb on + strand, within Psyr_5015 at 5956.551 kb on + strand, within Psyr_5015 at 5956.552 kb on - strand, within Psyr_5015 at 5956.552 kb on - strand, within Psyr_5015 at 5956.552 kb on - strand, within Psyr_5015 at 5956.780 kb on + strand, within Psyr_5015 at 5956.780 kb on + strand, within Psyr_5015 at 5956.976 kb on + strand, within Psyr_5015 at 5956.976 kb on + strand, within Psyr_5015 at 5956.976 kb on + strand, within Psyr_5015 at 5957.002 kb on + strand, within Psyr_5015 at 5957.136 kb on + strand, within Psyr_5015 at 5957.137 kb on - strand, within Psyr_5015 at 5957.142 kb on + strand, within Psyr_5015 at 5957.143 kb on - strand, within Psyr_5015 at 5957.149 kb on + strand, within Psyr_5015 at 5957.152 kb on + strand, within Psyr_5015 at 5957.152 kb on + strand, within Psyr_5015 at 5957.153 kb on - strand, within Psyr_5015 at 5957.153 kb on - strand, within Psyr_5015 at 5957.153 kb on - strand, within Psyr_5015 at 5957.153 kb on - strand, within Psyr_5015 at 5957.301 kb on - strand at 5957.301 kb on - strand at 5957.301 kb on - strand at 5957.301 kb on - strand at 5957.303 kb on - strand at 5957.308 kb on + strand at 5957.309 kb on - strand at 5957.309 kb on - strand at 5957.309 kb on - strand at 5957.319 kb on - strand at 5957.353 kb on + strand at 5957.361 kb on + strand at 5957.361 kb on + strand at 5957.362 kb on - strand at 5957.366 kb on - strand at 5957.383 kb on - strand at 5957.464 kb on + strand at 5957.493 kb on + strand at 5957.493 kb on + strand at 5957.494 kb on - strand at 5957.503 kb on + strand at 5957.512 kb on + strand at 5957.512 kb on + strand at 5957.524 kb on - strand at 5957.541 kb on + strand at 5957.542 kb on - strand at 5957.542 kb on - strand at 5957.641 kb on + strand at 5957.642 kb on - strand at 5957.642 kb on - strand at 5957.642 kb on - strand at 5957.642 kb on - strand at 5957.642 kb on - strand at 5957.645 kb on + strand at 5957.645 kb on + strand at 5957.645 kb on + strand at 5957.645 kb on + strand at 5957.645 kb on + strand at 5957.645 kb on + strand at 5957.645 kb on + strand at 5957.645 kb on + strand at 5957.646 kb on - strand at 5957.646 kb on - strand at 5957.646 kb on - strand at 5957.646 kb on - strand at 5957.646 kb on - strand at 5957.646 kb on - strand at 5957.646 kb on - strand at 5957.826 kb on + strand, within Psyr_5016 at 5957.826 kb on + strand, within Psyr_5016 at 5957.826 kb on + strand, within Psyr_5016 at 5957.827 kb on - strand, within Psyr_5016 at 5957.868 kb on + strand, within Psyr_5016 at 5957.868 kb on + strand, within Psyr_5016 at 5957.868 kb on + strand, within Psyr_5016 at 5957.868 kb on + strand, within Psyr_5016 at 5957.869 kb on - strand, within Psyr_5016 at 5957.869 kb on - strand, within Psyr_5016 at 5957.869 kb on - strand, within Psyr_5016 at 5958.247 kb on + strand, within Psyr_5016 at 5958.248 kb on - strand, within Psyr_5016
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Sinomenine 10 mM remove 5,955,374 + Psyr_5014 0.31 +0.2 5,955,374 + Psyr_5014 0.31 +0.7 5,955,375 - Psyr_5014 0.31 -0.7 5,955,821 + Psyr_5014 0.69 +0.2 5,955,821 + Psyr_5014 0.69 -0.9 5,955,821 + Psyr_5014 0.69 +0.6 5,955,821 + Psyr_5014 0.69 +1.0 5,955,821 + Psyr_5014 0.69 -0.1 5,955,822 - Psyr_5014 0.69 +0.7 5,955,822 - Psyr_5014 0.69 -0.3 5,955,822 - Psyr_5014 0.69 +0.9 5,955,944 + Psyr_5014 0.79 -0.4 5,955,953 + Psyr_5014 0.80 +1.0 5,955,953 + Psyr_5014 0.80 +0.9 5,955,953 + Psyr_5014 0.80 +0.4 5,955,953 + Psyr_5014 0.80 -0.3 5,955,953 + Psyr_5014 0.80 +0.7 5,955,953 + Psyr_5014 0.80 +1.4 5,955,953 + Psyr_5014 0.80 -0.2 5,955,953 + Psyr_5014 0.80 -0.0 5,955,954 - Psyr_5014 0.80 +0.9 5,955,954 - Psyr_5014 0.80 -0.6 5,955,954 - Psyr_5014 0.80 -0.1 5,956,231 + -0.1 5,956,231 + +0.9 5,956,266 - -0.3 5,956,342 + +0.6 5,956,359 - +1.2 5,956,549 + Psyr_5015 0.28 -0.9 5,956,549 + Psyr_5015 0.28 -1.1 5,956,549 + Psyr_5015 0.28 +2.8 5,956,550 - Psyr_5015 0.28 +1.3 5,956,550 - Psyr_5015 0.28 -0.4 5,956,550 - Psyr_5015 0.28 +0.1 5,956,550 - Psyr_5015 0.28 +1.4 5,956,551 + Psyr_5015 0.28 +0.6 5,956,551 + Psyr_5015 0.28 +0.5 5,956,551 + Psyr_5015 0.28 +0.6 5,956,551 + Psyr_5015 0.28 +0.1 5,956,552 - Psyr_5015 0.28 +1.4 5,956,552 - Psyr_5015 0.28 -0.4 5,956,552 - Psyr_5015 0.28 +0.2 5,956,780 + Psyr_5015 0.51 +0.6 5,956,780 + Psyr_5015 0.51 +0.8 5,956,976 + Psyr_5015 0.71 +0.9 5,956,976 + Psyr_5015 0.71 -0.2 5,956,976 + Psyr_5015 0.71 +0.4 5,957,002 + Psyr_5015 0.74 -0.1 5,957,136 + Psyr_5015 0.87 +1.1 5,957,137 - Psyr_5015 0.88 +1.4 5,957,142 + Psyr_5015 0.88 -0.8 5,957,143 - Psyr_5015 0.88 +0.0 5,957,149 + Psyr_5015 0.89 -0.6 5,957,152 + Psyr_5015 0.89 -0.3 5,957,152 + Psyr_5015 0.89 -0.7 5,957,153 - Psyr_5015 0.89 +1.0 5,957,153 - Psyr_5015 0.89 +0.4 5,957,153 - Psyr_5015 0.89 +1.0 5,957,153 - Psyr_5015 0.89 +0.9 5,957,301 - +0.1 5,957,301 - -0.3 5,957,301 - -2.6 5,957,301 - +0.5 5,957,303 - -0.6 5,957,308 + +0.3 5,957,309 - +0.8 5,957,309 - -0.6 5,957,309 - +0.4 5,957,319 - +0.5 5,957,353 + -0.1 5,957,361 + +0.5 5,957,361 + +0.1 5,957,362 - +1.7 5,957,366 - +0.6 5,957,383 - -1.1 5,957,464 + +0.1 5,957,493 + -0.5 5,957,493 + -1.4 5,957,494 - -0.3 5,957,503 + +0.2 5,957,512 + -0.7 5,957,512 + -0.2 5,957,524 - +0.7 5,957,541 + -0.6 5,957,542 - +1.2 5,957,542 - -0.4 5,957,641 + -0.0 5,957,642 - +0.4 5,957,642 - -0.4 5,957,642 - +0.1 5,957,642 - +2.4 5,957,642 - -0.4 5,957,645 + +0.3 5,957,645 + -2.9 5,957,645 + +0.0 5,957,645 + +0.2 5,957,645 + +0.3 5,957,645 + +0.2 5,957,645 + -0.2 5,957,645 + -1.4 5,957,646 - -0.6 5,957,646 - -0.4 5,957,646 - +0.1 5,957,646 - +0.6 5,957,646 - -0.2 5,957,646 - -1.3 5,957,646 - -1.3 5,957,826 + Psyr_5016 0.13 +0.5 5,957,826 + Psyr_5016 0.13 -0.1 5,957,826 + Psyr_5016 0.13 +0.6 5,957,827 - Psyr_5016 0.13 +1.2 5,957,868 + Psyr_5016 0.17 +0.7 5,957,868 + Psyr_5016 0.17 -1.2 5,957,868 + Psyr_5016 0.17 -0.9 5,957,868 + Psyr_5016 0.17 +1.0 5,957,869 - Psyr_5016 0.17 -0.2 5,957,869 - Psyr_5016 0.17 +0.1 5,957,869 - Psyr_5016 0.17 +0.4 5,958,247 + Psyr_5016 0.51 +0.5 5,958,248 - Psyr_5016 0.51 +0.8
Or see this region's nucleotide sequence