Experiment: KB with Sinomenine 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_3302 and Psyr_3303 are separated by 132 nucleotides Psyr_3303 and Psyr_3304 are separated by 17 nucleotides Psyr_3304 and Psyr_3305 overlap by 1 nucleotides
Psyr_3302: Psyr_3302 - mannose-6-phosphate isomerase, type 2 / mannose-1-phosphate guanylyltransferase (GDP), at 3,951,223 to 3,952,680
_3302
Psyr_3303: Psyr_3303 - Polysaccharide export protein, at 3,952,813 to 3,953,592
_3303
Psyr_3304: Psyr_3304 - Lipopolysaccharide biosynthesis, at 3,953,610 to 3,955,601
_3304
Psyr_3305: Psyr_3305 - Glycosyl transferase, group 1, at 3,955,601 to 3,956,779
_3305
Position (kb)
3953
3954
3955
3956 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3952.646 kb on - strand at 3952.646 kb on - strand at 3952.764 kb on + strand at 3952.765 kb on - strand at 3952.784 kb on + strand at 3952.784 kb on + strand at 3952.784 kb on + strand at 3952.785 kb on - strand at 3952.785 kb on - strand at 3952.785 kb on - strand at 3952.785 kb on - strand at 3952.841 kb on + strand at 3952.842 kb on - strand at 3952.864 kb on - strand at 3953.044 kb on + strand, within Psyr_3303 at 3953.044 kb on + strand, within Psyr_3303 at 3953.044 kb on + strand, within Psyr_3303 at 3953.044 kb on + strand, within Psyr_3303 at 3953.044 kb on + strand, within Psyr_3303 at 3953.045 kb on - strand, within Psyr_3303 at 3953.045 kb on - strand, within Psyr_3303 at 3953.045 kb on - strand, within Psyr_3303 at 3953.095 kb on + strand, within Psyr_3303 at 3953.095 kb on + strand, within Psyr_3303 at 3953.095 kb on + strand, within Psyr_3303 at 3953.096 kb on - strand, within Psyr_3303 at 3953.096 kb on - strand, within Psyr_3303 at 3953.096 kb on - strand, within Psyr_3303 at 3953.096 kb on - strand, within Psyr_3303 at 3953.096 kb on - strand, within Psyr_3303 at 3953.098 kb on - strand, within Psyr_3303 at 3953.374 kb on + strand, within Psyr_3303 at 3953.375 kb on - strand, within Psyr_3303 at 3953.383 kb on + strand, within Psyr_3303 at 3953.384 kb on - strand, within Psyr_3303 at 3953.384 kb on - strand, within Psyr_3303 at 3953.384 kb on - strand, within Psyr_3303 at 3953.396 kb on - strand, within Psyr_3303 at 3953.518 kb on + strand at 3953.518 kb on + strand at 3953.519 kb on - strand at 3953.570 kb on - strand at 3953.570 kb on - strand at 3953.570 kb on - strand at 3953.570 kb on - strand at 3953.590 kb on + strand at 3953.590 kb on + strand at 3953.590 kb on + strand at 3953.590 kb on + strand at 3953.590 kb on + strand at 3953.591 kb on - strand at 3953.591 kb on - strand at 3953.908 kb on - strand, within Psyr_3304 at 3953.908 kb on - strand, within Psyr_3304 at 3954.237 kb on + strand, within Psyr_3304 at 3954.237 kb on + strand, within Psyr_3304 at 3954.238 kb on - strand, within Psyr_3304 at 3954.279 kb on + strand, within Psyr_3304 at 3954.280 kb on - strand, within Psyr_3304 at 3954.300 kb on + strand, within Psyr_3304 at 3954.326 kb on + strand, within Psyr_3304 at 3954.326 kb on + strand, within Psyr_3304 at 3954.326 kb on + strand, within Psyr_3304 at 3954.327 kb on - strand, within Psyr_3304 at 3954.327 kb on - strand, within Psyr_3304 at 3954.327 kb on - strand, within Psyr_3304 at 3954.327 kb on - strand, within Psyr_3304 at 3954.649 kb on + strand, within Psyr_3304 at 3954.650 kb on - strand, within Psyr_3304 at 3954.650 kb on - strand, within Psyr_3304 at 3954.684 kb on + strand, within Psyr_3304 at 3954.684 kb on + strand, within Psyr_3304 at 3954.861 kb on + strand, within Psyr_3304 at 3954.861 kb on + strand, within Psyr_3304 at 3954.870 kb on + strand, within Psyr_3304 at 3954.930 kb on - strand, within Psyr_3304 at 3954.930 kb on - strand, within Psyr_3304 at 3954.932 kb on + strand, within Psyr_3304 at 3955.044 kb on + strand, within Psyr_3304 at 3955.045 kb on - strand, within Psyr_3304 at 3955.053 kb on + strand, within Psyr_3304 at 3955.189 kb on - strand, within Psyr_3304 at 3955.213 kb on + strand, within Psyr_3304 at 3955.213 kb on + strand, within Psyr_3304 at 3955.213 kb on + strand, within Psyr_3304 at 3955.241 kb on + strand, within Psyr_3304 at 3955.241 kb on + strand, within Psyr_3304 at 3955.608 kb on + strand at 3955.705 kb on + strand at 3955.706 kb on - strand at 3956.180 kb on + strand, within Psyr_3305 at 3956.180 kb on + strand, within Psyr_3305 at 3956.180 kb on + strand, within Psyr_3305 at 3956.333 kb on + strand, within Psyr_3305 at 3956.334 kb on - strand, within Psyr_3305 at 3956.334 kb on - strand, within Psyr_3305 at 3956.548 kb on - strand, within Psyr_3305
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Sinomenine 10 mM remove 3,952,646 - -0.4 3,952,646 - -0.1 3,952,764 + +0.2 3,952,765 - +0.6 3,952,784 + -0.7 3,952,784 + +1.1 3,952,784 + +1.6 3,952,785 - +0.1 3,952,785 - +0.3 3,952,785 - -0.8 3,952,785 - +0.7 3,952,841 + +1.4 3,952,842 - -1.1 3,952,864 - -1.3 3,953,044 + Psyr_3303 0.30 +0.3 3,953,044 + Psyr_3303 0.30 +0.2 3,953,044 + Psyr_3303 0.30 -1.8 3,953,044 + Psyr_3303 0.30 -0.9 3,953,044 + Psyr_3303 0.30 +0.4 3,953,045 - Psyr_3303 0.30 -0.9 3,953,045 - Psyr_3303 0.30 -1.3 3,953,045 - Psyr_3303 0.30 +0.1 3,953,095 + Psyr_3303 0.36 +1.4 3,953,095 + Psyr_3303 0.36 +0.8 3,953,095 + Psyr_3303 0.36 +0.2 3,953,096 - Psyr_3303 0.36 -0.5 3,953,096 - Psyr_3303 0.36 +0.5 3,953,096 - Psyr_3303 0.36 -1.2 3,953,096 - Psyr_3303 0.36 +0.8 3,953,096 - Psyr_3303 0.36 -0.1 3,953,098 - Psyr_3303 0.37 +1.2 3,953,374 + Psyr_3303 0.72 -0.9 3,953,375 - Psyr_3303 0.72 +0.6 3,953,383 + Psyr_3303 0.73 -1.9 3,953,384 - Psyr_3303 0.73 +0.4 3,953,384 - Psyr_3303 0.73 +1.2 3,953,384 - Psyr_3303 0.73 -0.4 3,953,396 - Psyr_3303 0.75 +0.9 3,953,518 + +2.6 3,953,518 + +0.4 3,953,519 - -0.4 3,953,570 - +0.5 3,953,570 - -0.8 3,953,570 - +0.4 3,953,570 - +0.2 3,953,590 + +1.5 3,953,590 + +0.5 3,953,590 + -0.4 3,953,590 + -0.1 3,953,590 + +0.6 3,953,591 - -2.3 3,953,591 - +0.6 3,953,908 - Psyr_3304 0.15 -1.9 3,953,908 - Psyr_3304 0.15 -1.7 3,954,237 + Psyr_3304 0.31 -0.6 3,954,237 + Psyr_3304 0.31 -0.2 3,954,238 - Psyr_3304 0.32 +0.1 3,954,279 + Psyr_3304 0.34 +0.1 3,954,280 - Psyr_3304 0.34 -0.2 3,954,300 + Psyr_3304 0.35 +0.5 3,954,326 + Psyr_3304 0.36 -0.2 3,954,326 + Psyr_3304 0.36 +0.6 3,954,326 + Psyr_3304 0.36 +0.1 3,954,327 - Psyr_3304 0.36 -0.2 3,954,327 - Psyr_3304 0.36 -1.1 3,954,327 - Psyr_3304 0.36 -0.0 3,954,327 - Psyr_3304 0.36 +0.3 3,954,649 + Psyr_3304 0.52 -0.0 3,954,650 - Psyr_3304 0.52 -0.0 3,954,650 - Psyr_3304 0.52 +1.5 3,954,684 + Psyr_3304 0.54 -0.4 3,954,684 + Psyr_3304 0.54 -0.1 3,954,861 + Psyr_3304 0.63 +2.1 3,954,861 + Psyr_3304 0.63 -0.9 3,954,870 + Psyr_3304 0.63 -0.5 3,954,930 - Psyr_3304 0.66 -0.8 3,954,930 - Psyr_3304 0.66 -1.9 3,954,932 + Psyr_3304 0.66 +0.8 3,955,044 + Psyr_3304 0.72 -0.5 3,955,045 - Psyr_3304 0.72 -0.7 3,955,053 + Psyr_3304 0.72 -2.1 3,955,189 - Psyr_3304 0.79 +0.1 3,955,213 + Psyr_3304 0.80 +0.3 3,955,213 + Psyr_3304 0.80 -0.8 3,955,213 + Psyr_3304 0.80 +0.4 3,955,241 + Psyr_3304 0.82 -0.7 3,955,241 + Psyr_3304 0.82 -0.3 3,955,608 + -0.3 3,955,705 + +0.4 3,955,706 - +0.5 3,956,180 + Psyr_3305 0.49 +0.3 3,956,180 + Psyr_3305 0.49 +0.7 3,956,180 + Psyr_3305 0.49 -0.8 3,956,333 + Psyr_3305 0.62 -2.1 3,956,334 - Psyr_3305 0.62 -0.5 3,956,334 - Psyr_3305 0.62 -0.8 3,956,548 - Psyr_3305 0.80 -1.2
Or see this region's nucleotide sequence