Experiment: KB with Sinomenine 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2413 and Psyr_2414 are separated by 70 nucleotides Psyr_2414 and Psyr_2415 are separated by 319 nucleotides Psyr_2415 and Psyr_2416 are separated by 95 nucleotides
Psyr_2413: Psyr_2413 - succinate semialdehyde dehydrogenase, at 2,796,408 to 2,797,838
_2413
Psyr_2414: Psyr_2414 - murein-DD-endopeptidase, Serine peptidase, MEROPS family S11, at 2,797,909 to 2,798,793
_2414
Psyr_2415: Psyr_2415 - hypothetical protein, at 2,799,113 to 2,799,673
_2415
Psyr_2416: Psyr_2416 - conserved hypothetical protein, at 2,799,769 to 2,799,951
_2416
Position (kb)
2797
2798
2799 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 2797.398 kb on - strand, within Psyr_2413 at 2797.398 kb on - strand, within Psyr_2413 at 2797.588 kb on + strand, within Psyr_2413 at 2797.589 kb on - strand, within Psyr_2413 at 2797.589 kb on - strand, within Psyr_2413 at 2797.589 kb on - strand, within Psyr_2413 at 2797.638 kb on + strand, within Psyr_2413 at 2797.638 kb on + strand, within Psyr_2413 at 2797.639 kb on - strand, within Psyr_2413 at 2797.639 kb on - strand, within Psyr_2413 at 2797.639 kb on - strand, within Psyr_2413 at 2797.639 kb on - strand, within Psyr_2413 at 2797.683 kb on + strand, within Psyr_2413 at 2797.683 kb on + strand, within Psyr_2413 at 2797.683 kb on + strand, within Psyr_2413 at 2797.683 kb on + strand, within Psyr_2413 at 2797.684 kb on - strand, within Psyr_2413 at 2797.804 kb on - strand at 2797.856 kb on - strand at 2797.964 kb on + strand at 2797.965 kb on - strand at 2798.048 kb on + strand, within Psyr_2414 at 2798.049 kb on - strand, within Psyr_2414 at 2798.049 kb on - strand, within Psyr_2414 at 2798.049 kb on - strand, within Psyr_2414 at 2798.213 kb on + strand, within Psyr_2414 at 2798.214 kb on - strand, within Psyr_2414 at 2798.214 kb on - strand, within Psyr_2414 at 2798.214 kb on - strand, within Psyr_2414 at 2798.214 kb on - strand, within Psyr_2414 at 2798.214 kb on - strand, within Psyr_2414 at 2798.330 kb on + strand, within Psyr_2414 at 2798.331 kb on - strand, within Psyr_2414 at 2798.331 kb on - strand, within Psyr_2414 at 2798.331 kb on - strand, within Psyr_2414 at 2798.357 kb on + strand, within Psyr_2414 at 2798.358 kb on - strand, within Psyr_2414 at 2798.358 kb on - strand, within Psyr_2414 at 2798.675 kb on + strand, within Psyr_2414 at 2798.676 kb on - strand, within Psyr_2414 at 2798.803 kb on - strand at 2798.807 kb on - strand at 2798.807 kb on - strand at 2798.807 kb on - strand at 2798.812 kb on + strand at 2798.812 kb on + strand at 2798.813 kb on - strand at 2798.813 kb on - strand at 2798.862 kb on + strand at 2798.863 kb on - strand at 2798.873 kb on - strand at 2798.906 kb on + strand at 2798.907 kb on - strand at 2798.925 kb on - strand at 2798.925 kb on - strand at 2798.958 kb on - strand at 2798.958 kb on - strand at 2798.958 kb on - strand at 2798.981 kb on + strand at 2798.982 kb on - strand at 2798.982 kb on - strand at 2798.992 kb on + strand at 2798.993 kb on - strand at 2799.199 kb on + strand, within Psyr_2415 at 2799.324 kb on + strand, within Psyr_2415 at 2799.324 kb on + strand, within Psyr_2415 at 2799.325 kb on - strand, within Psyr_2415 at 2799.325 kb on - strand, within Psyr_2415 at 2799.325 kb on - strand, within Psyr_2415 at 2799.344 kb on + strand, within Psyr_2415 at 2799.363 kb on - strand, within Psyr_2415 at 2799.449 kb on + strand, within Psyr_2415 at 2799.449 kb on + strand, within Psyr_2415 at 2799.449 kb on + strand, within Psyr_2415 at 2799.450 kb on - strand, within Psyr_2415 at 2799.450 kb on - strand, within Psyr_2415 at 2799.465 kb on - strand, within Psyr_2415 at 2799.527 kb on + strand, within Psyr_2415
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Sinomenine 10 mM remove 2,797,398 - Psyr_2413 0.69 -0.1 2,797,398 - Psyr_2413 0.69 +0.3 2,797,588 + Psyr_2413 0.82 +0.7 2,797,589 - Psyr_2413 0.83 +0.7 2,797,589 - Psyr_2413 0.83 +1.1 2,797,589 - Psyr_2413 0.83 -0.6 2,797,638 + Psyr_2413 0.86 -1.4 2,797,638 + Psyr_2413 0.86 +0.1 2,797,639 - Psyr_2413 0.86 -1.1 2,797,639 - Psyr_2413 0.86 +2.1 2,797,639 - Psyr_2413 0.86 -0.3 2,797,639 - Psyr_2413 0.86 -0.4 2,797,683 + Psyr_2413 0.89 +0.1 2,797,683 + Psyr_2413 0.89 +0.1 2,797,683 + Psyr_2413 0.89 -2.4 2,797,683 + Psyr_2413 0.89 +0.3 2,797,684 - Psyr_2413 0.89 +0.1 2,797,804 - -0.6 2,797,856 - +0.5 2,797,964 + +0.6 2,797,965 - -0.3 2,798,048 + Psyr_2414 0.16 +1.1 2,798,049 - Psyr_2414 0.16 +1.4 2,798,049 - Psyr_2414 0.16 +2.1 2,798,049 - Psyr_2414 0.16 +0.2 2,798,213 + Psyr_2414 0.34 +0.5 2,798,214 - Psyr_2414 0.34 +0.3 2,798,214 - Psyr_2414 0.34 +0.2 2,798,214 - Psyr_2414 0.34 +0.1 2,798,214 - Psyr_2414 0.34 -0.0 2,798,214 - Psyr_2414 0.34 +1.1 2,798,330 + Psyr_2414 0.48 -0.4 2,798,331 - Psyr_2414 0.48 +1.3 2,798,331 - Psyr_2414 0.48 +2.3 2,798,331 - Psyr_2414 0.48 -0.2 2,798,357 + Psyr_2414 0.51 +1.3 2,798,358 - Psyr_2414 0.51 -0.6 2,798,358 - Psyr_2414 0.51 -0.1 2,798,675 + Psyr_2414 0.87 +0.3 2,798,676 - Psyr_2414 0.87 +0.3 2,798,803 - +0.6 2,798,807 - -1.5 2,798,807 - +0.5 2,798,807 - -0.4 2,798,812 + -0.4 2,798,812 + +0.4 2,798,813 - +1.2 2,798,813 - -0.7 2,798,862 + -0.9 2,798,863 - +0.8 2,798,873 - +0.4 2,798,906 + -0.5 2,798,907 - -0.7 2,798,925 - -0.2 2,798,925 - +0.2 2,798,958 - -0.4 2,798,958 - +1.5 2,798,958 - +1.1 2,798,981 + +3.9 2,798,982 - -0.6 2,798,982 - +1.5 2,798,992 + +0.5 2,798,993 - -0.3 2,799,199 + Psyr_2415 0.15 -0.1 2,799,324 + Psyr_2415 0.38 +0.1 2,799,324 + Psyr_2415 0.38 +0.2 2,799,325 - Psyr_2415 0.38 -0.1 2,799,325 - Psyr_2415 0.38 +0.7 2,799,325 - Psyr_2415 0.38 -1.7 2,799,344 + Psyr_2415 0.41 -2.5 2,799,363 - Psyr_2415 0.45 -0.3 2,799,449 + Psyr_2415 0.60 +1.6 2,799,449 + Psyr_2415 0.60 -0.2 2,799,449 + Psyr_2415 0.60 -0.2 2,799,450 - Psyr_2415 0.60 +0.5 2,799,450 - Psyr_2415 0.60 -4.0 2,799,465 - Psyr_2415 0.63 +1.1 2,799,527 + Psyr_2415 0.74 +0.3
Or see this region's nucleotide sequence