Experiment: KB with Sinomenine 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1062 and Psyr_1063 are separated by 100 nucleotides Psyr_1063 and Psyr_1064 are separated by 815 nucleotides
Psyr_1062: Psyr_1062 - alginate biosynthesis protein Alg8, at 1,203,142 to 1,204,623
_1062
Psyr_1063: Psyr_1063 - GDP-mannose 6-dehydrogenase, at 1,204,724 to 1,206,040
_1063
Psyr_1064: Psyr_1064 - Protein of unknown function DUF328, at 1,206,856 to 1,207,635
_1064
Position (kb) 1204
1205
1206
1207 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 1203.950 kb on + strand, within Psyr_1062 at 1203.950 kb on + strand, within Psyr_1062 at 1203.951 kb on - strand, within Psyr_1062 at 1204.304 kb on + strand, within Psyr_1062 at 1204.304 kb on + strand, within Psyr_1062 at 1204.305 kb on - strand, within Psyr_1062 at 1204.305 kb on - strand, within Psyr_1062 at 1204.424 kb on + strand, within Psyr_1062 at 1204.502 kb on - strand at 1204.502 kb on - strand at 1204.502 kb on - strand at 1204.503 kb on + strand at 1204.503 kb on + strand at 1204.504 kb on - strand at 1204.504 kb on - strand at 1204.537 kb on - strand at 1204.571 kb on - strand at 1204.571 kb on - strand at 1204.574 kb on + strand at 1204.574 kb on + strand at 1204.574 kb on + strand at 1204.574 kb on + strand at 1204.575 kb on - strand at 1204.575 kb on - strand at 1204.597 kb on + strand at 1204.635 kb on + strand at 1204.635 kb on + strand at 1204.635 kb on + strand at 1204.636 kb on - strand at 1204.735 kb on + strand at 1204.735 kb on + strand at 1204.938 kb on + strand, within Psyr_1063 at 1204.938 kb on + strand, within Psyr_1063 at 1204.938 kb on + strand, within Psyr_1063 at 1204.939 kb on - strand, within Psyr_1063 at 1204.939 kb on - strand, within Psyr_1063 at 1204.968 kb on + strand, within Psyr_1063 at 1204.968 kb on + strand, within Psyr_1063 at 1205.033 kb on + strand, within Psyr_1063 at 1205.034 kb on - strand, within Psyr_1063 at 1205.109 kb on + strand, within Psyr_1063 at 1205.153 kb on - strand, within Psyr_1063 at 1205.271 kb on + strand, within Psyr_1063 at 1205.271 kb on + strand, within Psyr_1063 at 1205.271 kb on + strand, within Psyr_1063 at 1205.271 kb on + strand, within Psyr_1063 at 1205.271 kb on + strand, within Psyr_1063 at 1205.271 kb on + strand, within Psyr_1063 at 1205.271 kb on + strand, within Psyr_1063 at 1205.271 kb on + strand, within Psyr_1063 at 1205.271 kb on + strand, within Psyr_1063 at 1205.271 kb on + strand, within Psyr_1063 at 1205.271 kb on + strand, within Psyr_1063 at 1205.271 kb on + strand, within Psyr_1063 at 1205.271 kb on + strand, within Psyr_1063 at 1205.271 kb on + strand, within Psyr_1063 at 1205.271 kb on + strand, within Psyr_1063 at 1205.271 kb on + strand, within Psyr_1063 at 1205.272 kb on - strand, within Psyr_1063 at 1205.272 kb on - strand, within Psyr_1063 at 1205.272 kb on - strand, within Psyr_1063 at 1205.272 kb on - strand, within Psyr_1063 at 1205.272 kb on - strand, within Psyr_1063 at 1205.272 kb on - strand, within Psyr_1063 at 1205.272 kb on - strand, within Psyr_1063 at 1205.272 kb on - strand, within Psyr_1063 at 1205.275 kb on - strand, within Psyr_1063 at 1205.409 kb on + strand, within Psyr_1063 at 1205.409 kb on + strand, within Psyr_1063 at 1205.410 kb on - strand, within Psyr_1063 at 1205.470 kb on - strand, within Psyr_1063 at 1205.539 kb on - strand, within Psyr_1063 at 1205.539 kb on - strand, within Psyr_1063 at 1205.554 kb on + strand, within Psyr_1063 at 1205.686 kb on + strand, within Psyr_1063 at 1205.748 kb on + strand, within Psyr_1063 at 1205.748 kb on + strand, within Psyr_1063 at 1205.749 kb on - strand, within Psyr_1063 at 1205.749 kb on - strand, within Psyr_1063 at 1205.839 kb on + strand, within Psyr_1063 at 1205.906 kb on - strand, within Psyr_1063 at 1205.963 kb on + strand at 1205.963 kb on + strand at 1205.964 kb on - strand at 1205.964 kb on - strand at 1206.012 kb on + strand at 1206.026 kb on + strand at 1206.026 kb on + strand at 1206.026 kb on + strand at 1206.027 kb on - strand at 1206.067 kb on + strand at 1206.151 kb on + strand at 1206.179 kb on + strand at 1206.200 kb on + strand at 1206.200 kb on + strand at 1206.201 kb on - strand at 1206.201 kb on - strand at 1206.217 kb on + strand at 1206.217 kb on + strand at 1206.260 kb on + strand at 1206.388 kb on + strand at 1206.388 kb on + strand at 1206.388 kb on + strand at 1206.388 kb on + strand at 1206.402 kb on + strand at 1206.402 kb on + strand at 1206.479 kb on + strand at 1206.480 kb on - strand at 1206.480 kb on - strand at 1206.480 kb on - strand at 1206.480 kb on - strand at 1206.491 kb on + strand at 1206.492 kb on - strand at 1206.492 kb on - strand at 1206.521 kb on + strand at 1206.522 kb on - strand at 1206.522 kb on - strand at 1206.543 kb on + strand at 1206.543 kb on + strand at 1206.598 kb on + strand at 1206.598 kb on + strand at 1206.598 kb on + strand at 1206.599 kb on - strand at 1206.642 kb on + strand at 1206.642 kb on + strand at 1206.750 kb on - strand at 1206.809 kb on + strand at 1206.857 kb on + strand at 1206.857 kb on + strand at 1206.858 kb on - strand at 1206.931 kb on + strand at 1206.932 kb on - strand at 1206.932 kb on - strand at 1206.932 kb on - strand at 1206.946 kb on + strand, within Psyr_1064 at 1206.947 kb on - strand, within Psyr_1064 at 1206.947 kb on - strand, within Psyr_1064 at 1206.976 kb on + strand, within Psyr_1064 at 1206.976 kb on + strand, within Psyr_1064 at 1206.976 kb on + strand, within Psyr_1064 at 1206.976 kb on + strand, within Psyr_1064 at 1206.977 kb on - strand, within Psyr_1064 at 1206.977 kb on - strand, within Psyr_1064 at 1206.977 kb on - strand, within Psyr_1064 at 1207.017 kb on - strand, within Psyr_1064 at 1207.017 kb on - strand, within Psyr_1064 at 1207.034 kb on + strand, within Psyr_1064 at 1207.035 kb on - strand, within Psyr_1064
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Sinomenine 10 mM remove 1,203,950 + Psyr_1062 0.55 +0.5 1,203,950 + Psyr_1062 0.55 -0.1 1,203,951 - Psyr_1062 0.55 -0.4 1,204,304 + Psyr_1062 0.78 +0.1 1,204,304 + Psyr_1062 0.78 -0.5 1,204,305 - Psyr_1062 0.78 -2.0 1,204,305 - Psyr_1062 0.78 +0.1 1,204,424 + Psyr_1062 0.87 +0.1 1,204,502 - -0.6 1,204,502 - -0.5 1,204,502 - +0.9 1,204,503 + +0.4 1,204,503 + -0.1 1,204,504 - -0.4 1,204,504 - -1.1 1,204,537 - -0.6 1,204,571 - +1.1 1,204,571 - -0.3 1,204,574 + +0.0 1,204,574 + +0.4 1,204,574 + +0.2 1,204,574 + +0.2 1,204,575 - -1.2 1,204,575 - +0.6 1,204,597 + +0.2 1,204,635 + -0.6 1,204,635 + +0.4 1,204,635 + -1.1 1,204,636 - -0.2 1,204,735 + +0.2 1,204,735 + -2.7 1,204,938 + Psyr_1063 0.16 +0.3 1,204,938 + Psyr_1063 0.16 -0.4 1,204,938 + Psyr_1063 0.16 -0.4 1,204,939 - Psyr_1063 0.16 +0.9 1,204,939 - Psyr_1063 0.16 +0.4 1,204,968 + Psyr_1063 0.19 +0.5 1,204,968 + Psyr_1063 0.19 -0.7 1,205,033 + Psyr_1063 0.23 +0.5 1,205,034 - Psyr_1063 0.24 +1.6 1,205,109 + Psyr_1063 0.29 +0.0 1,205,153 - Psyr_1063 0.33 -1.4 1,205,271 + Psyr_1063 0.42 -0.3 1,205,271 + Psyr_1063 0.42 +0.4 1,205,271 + Psyr_1063 0.42 -0.1 1,205,271 + Psyr_1063 0.42 +0.4 1,205,271 + Psyr_1063 0.42 +0.3 1,205,271 + Psyr_1063 0.42 +0.9 1,205,271 + Psyr_1063 0.42 +0.6 1,205,271 + Psyr_1063 0.42 +0.7 1,205,271 + Psyr_1063 0.42 +1.6 1,205,271 + Psyr_1063 0.42 -0.4 1,205,271 + Psyr_1063 0.42 +0.8 1,205,271 + Psyr_1063 0.42 +0.1 1,205,271 + Psyr_1063 0.42 +0.1 1,205,271 + Psyr_1063 0.42 +0.6 1,205,271 + Psyr_1063 0.42 +0.7 1,205,271 + Psyr_1063 0.42 -0.3 1,205,272 - Psyr_1063 0.42 +0.8 1,205,272 - Psyr_1063 0.42 -0.3 1,205,272 - Psyr_1063 0.42 +0.9 1,205,272 - Psyr_1063 0.42 +0.9 1,205,272 - Psyr_1063 0.42 -0.9 1,205,272 - Psyr_1063 0.42 +0.8 1,205,272 - Psyr_1063 0.42 +0.4 1,205,272 - Psyr_1063 0.42 +0.5 1,205,275 - Psyr_1063 0.42 -0.4 1,205,409 + Psyr_1063 0.52 -0.8 1,205,409 + Psyr_1063 0.52 +0.9 1,205,410 - Psyr_1063 0.52 +0.8 1,205,470 - Psyr_1063 0.57 +0.0 1,205,539 - Psyr_1063 0.62 -0.8 1,205,539 - Psyr_1063 0.62 -0.0 1,205,554 + Psyr_1063 0.63 +2.9 1,205,686 + Psyr_1063 0.73 +1.9 1,205,748 + Psyr_1063 0.78 -0.6 1,205,748 + Psyr_1063 0.78 -0.2 1,205,749 - Psyr_1063 0.78 +1.2 1,205,749 - Psyr_1063 0.78 +0.9 1,205,839 + Psyr_1063 0.85 -0.2 1,205,906 - Psyr_1063 0.90 +0.4 1,205,963 + -2.9 1,205,963 + +1.0 1,205,964 - -0.7 1,205,964 - -0.1 1,206,012 + -1.8 1,206,026 + -0.2 1,206,026 + -0.5 1,206,026 + -0.1 1,206,027 - -0.3 1,206,067 + +0.1 1,206,151 + +0.4 1,206,179 + +0.4 1,206,200 + +0.1 1,206,200 + -0.8 1,206,201 - +0.4 1,206,201 - +0.4 1,206,217 + +1.2 1,206,217 + -1.5 1,206,260 + -0.1 1,206,388 + -1.5 1,206,388 + -1.1 1,206,388 + -0.6 1,206,388 + -0.4 1,206,402 + +0.1 1,206,402 + -0.5 1,206,479 + +2.1 1,206,480 - -0.9 1,206,480 - +0.4 1,206,480 - -1.5 1,206,480 - +0.2 1,206,491 + +0.4 1,206,492 - +0.3 1,206,492 - +1.9 1,206,521 + +0.6 1,206,522 - +0.7 1,206,522 - -2.4 1,206,543 + -0.2 1,206,543 + -0.1 1,206,598 + +0.3 1,206,598 + -2.1 1,206,598 + +0.4 1,206,599 - +0.5 1,206,642 + -0.7 1,206,642 + -0.8 1,206,750 - +1.6 1,206,809 + -0.5 1,206,857 + +1.9 1,206,857 + -0.1 1,206,858 - -0.1 1,206,931 + -0.4 1,206,932 - -0.2 1,206,932 - -1.9 1,206,932 - +0.7 1,206,946 + Psyr_1064 0.12 -0.5 1,206,947 - Psyr_1064 0.12 -1.5 1,206,947 - Psyr_1064 0.12 +0.2 1,206,976 + Psyr_1064 0.15 -0.2 1,206,976 + Psyr_1064 0.15 +0.5 1,206,976 + Psyr_1064 0.15 -1.5 1,206,976 + Psyr_1064 0.15 +0.2 1,206,977 - Psyr_1064 0.16 +0.3 1,206,977 - Psyr_1064 0.16 +0.3 1,206,977 - Psyr_1064 0.16 +0.5 1,207,017 - Psyr_1064 0.21 -0.2 1,207,017 - Psyr_1064 0.21 +0.4 1,207,034 + Psyr_1064 0.23 +0.2 1,207,035 - Psyr_1064 0.23 -0.2
Or see this region's nucleotide sequence